Systems and methods are provided for counting particles in a fluid flow. In an aspect, coordinates of particles are obtained from video data of particles in a fluid, the video data made up of a sequence of image frames. The particle positions are linked in each pair of consecutive image frames of the video data. The linked particle positions are used to calculate particle trajectories through sequential image frames of the video data, and the particles are counted based on the particle trajectory. In another aspect, the particle positons within each image frame are transformed to estimated positions within a common coordinate frame. The estimated particle positions of a particle are grouped into a cluster center, and the particle count is calculated based on the cluster centers.

The present disclosure provides micro-array devices for capturing cells in blood and methods of their use. In some aspects, a method for counting cells in a blood sample is provided, the method comprising applying a blood sample onto a CNT device; allowing cells in the blood sample to differentially settle on the CNT device, and identifying and counting cells of preselected type in the blood sample.

A method of analysing and identifying particles in an input fluid by electrical field analysis preferably in combination with imaging analysis and towards establishing characterises of the particles that can be compared with characteristics of known particles, preferably biologic particles such as bacteria and viruses. Preferably in a focusing step, the particles are focused as in a microfluidic particle sorting cartridge followed by electrical field analysis to determine impedance data. Preferably, the electrical field analysis is carried out in a water and alcohol solution.

The present application is directed to a system for collecting or extruding a cell-containing biological material. The system includes an extrusion/collection device comprising: an orifice and a chamber operably connected to the orifice and an electrical impedance spectroscopy (EIS) device, operably connected to the extrusion/collection device. The EIS device monitors biologically relevant attributes of a cell-containing biological material as it is extruded from or collected into the extrusion/collection device. Also disclosed are methods of using the system disclosed herein.

A vaporized aerosol, particle, and gas detection network includes an entry unit that includes a trigger sensor configured to detect a triggering event in an environment. Further the trigger sensor generates a detection signal in response to the detected triggering event in the environment. The entry unit also includes an entry unit housing configured to enclose at least a portion of the trigger sensor. The network additionally includes a detection unit communicatively connected to the entry unit that includes a particle sensor configured to detect a particle count of the environment in response to the generation of the detection signal. The detection unit also has a detection unit housing configured to enclose at least a portion of the particle sensor.

The systems and methods of the present disclosure are directed to ultrasound-based concentration measurement techniques in which both scatterer count and image volume are measured concurrently to provide absolute concentration measurements. In particular, through the techniques of the present disclosure, the effective thickness of an ultrasound beam can be determined based on the spreading of individual scatterers within ultrasound images. Based on the effective thickness of the ultrasound beam, the volume of the image and, thus, the concentration of particles in the image can be determined directly, without the need for estimation, approximation, or use of a reference sample.

A fractionated photoacoustic flow cytometry (PAFC) system and methods for the in vivo detection of target objects in biofluidic systems (e.g., blood, lymph, urine, or cerebrospinal fluid) of a living organism is described. The fractionated system includes a fractionated laser system, a fractionated optical system, a fractionated acoustic system, and combinations thereof. The fractionated laser system includes at least one laser or laser array for pulsing a target object within the circulatory vessel with fractionated focused laser beams. The fractionated optical system separates one or several laser beams into multiple beams in a spatial configuration on the skin above the circulatory vessel of the living organism. The fractionated acoustic system includes multiple focused ultrasound transducers for receiving photoacoustic signals emitted by the target object in response to the fractionated laser beams.

A slurry analysis system (14) for estimating a first characteristic of a slurry (12) having a plurality of particles (18) suspended in a dispersion medium (20) can include a flow restriction assembly (40); a sensor assembly (43) that senses a sensed condition of the slurry (12) as it flows through the flow restriction assembly (40); and a control and analysis system (26) that estimates the first characteristic of the slurry (12) based on the sensed condition. Further, the control and analysis system (26) can select a selected clogging behavior using the sensed condition, and estimate the first characteristic based on the selected clogging behavior.

A method of controlling a blood analyzer for measuring platelets is provided. The method comprises: determining a relationship between at least one first measurement value obtained by detecting platelets in at least one previous test by an electrical type detector of the blood analyzer and at least one second measurement value obtained by detecting the platelets in the at least one previous test by an optical type detector of the blood analyzer, and controlling the blood analyzer to prepare the first and/or second measurement sample for a current test according to the determined relationship.

Among others, the present invention provides apparatus for interacting with a biological subject to detect circulating tumor cells therein, comprising one device for sending a signal to the biological subject and optionally receiving a response to the signal from the biological entity.