A micro-nano particle detection device and method are disclosed. The device includes a sample chamber and at least two measurement chambers, where at least one through hole is formed between each measurement chamber and the sample chamber, each measurement chamber is communicated with the sample chamber only through the through hole, a common electrode is arranged in the sample chamber, a measurement electrode is arranged in each measurement chamber respectively, a first end of the sample chamber is provided with a first liquid driving device, and the common electrode is grounded.

A particle separating device includes at least three liquid chambers adapted to store a liquid therein; at least two liquid passages, each connecting adjacent two of the liquid chambers; an inlet adapted to introduce a liquid in which multiple particles of different sizes are dispersed into one of the liquid chambers; and at least two electrodes disposed inside at least two of the liquid chambers, respectively, the electrodes adapted to apply different electrical potentials to the liquid. The cross-sectional areas of the at least two liquid passages are different from each other.

A particle analysis device includes multiple stacked plates joined together; an upper liquid space adapted to store a first liquid; a lower liquid space adapted to store a second liquid; a connection pore connecting the upper liquid space to the lower liquid space; a first hole extending from the top surface to the upper liquid space, the first liquid flowing through the first hole; and a second hole extending from the top surface to the lower liquid space, the second liquid flowing through the second hole. A first electrode and a second electrode that are sheets are pinched between two of the plates. The first electrode applies an electric potential to the first liquid in the upper liquid space through the first hole, whereas the second electrode applies an electric potential to the second liquid in the lower liquid space through the second hole. The particle analysis device further includes a first electrode-rod-insertion hole extending from the top surface to the first electrode, and a second electrode-rod-insertion hole extending from the top surface to the second electrode. The first electrode and the second electrode are not exposed at any side surface of the particle analysis device.

A particle analysis device includes a liquid space adapted to store a liquid; a chip disposed above the liquid space, the chip having a connection pore extending vertically and communicating with the liquid space; an upper hole disposed above the chip, the upper hole extending vertically and communicating with the connection pore; a first electrode adapted to apply an electric potential to a liquid in the upper hole; and a second electrode adapted to apply an electric potential to the liquid in the liquid space. The upper hole having a diameter that is equal to or greater than the maximum width of the connection pore, and the entirety of the connection pore falling within the range of the upper hole.

A system and method for electrical label-free detection of single protein molecules via a nanoscale electrode based on detecting the transient potential change of the floating nanoelectrode, which works for both large and small molecules. The system can also be applied to study the interactions of molecules with molecular receptors on the surface of the nanoscale electrode. The motion and dynamics of the protein near the nanoscale electrode can be detected with high precision in real time based on their intrinsic charges by the potentiometric method using a differential amplifier. The nanoelectrode can be integrated into a microfluidic device for biosensing applications.

A number analyzing method, a number analyzing device, and a storage medium for number analysis are disclosed, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device in accordance with passage of subject particles through a through-hole. Also, a particle type distribution estimating program is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.

The disclosure provides a method of manipulating droplets in an electro-wetting on dielectric (EWOD) device. Electro-wetting electrodes of the EWOD device are selectively actuated to: cause first and second droplets in a fluid medium in the fluid chamber of the EWOD device to contact each other to form a droplet interface bilayer, the first droplet containing fluid of a first composition including a first solute species and the second droplet containing fluid of a second composition different to the first composition, maintain the first and second droplets contacting each other to maintain the droplet interface bilayer and thereby allow the first solute species to pass from the first droplet to the second droplet via the DIB; and cause the first droplet to separate from the second droplet. This method aspect results in transfer of solute from the first droplet to the second droplet. This provides a convenient way of altering the concentration of a particular component or components in a fluid droplet within an EWOD device. This allows, for example, an undesired solute species to be extracted from a reaction droplet or the undesired solute species to be diluted in the reaction droplet before the droplet undergoes further reaction steps.

A method having the steps of obtaining prepared image data captured by an image sensor receiving light propagated across a sample volume, containing a fluid possibly comprising microscopic objects of foreign origin, while illuminating the sample volume by coherent light. The prepared image data comprising, for a microscopic object, a prepared hologram pattern with prepared spatially alternating intensity formed by the interference fringes; providing filtered image data, comprising automatically filtering the prepared image data by an edge enhancing filter. the filtered image data comprising, for a prepared hologram pattern, a filtered hologram pattern. The presence of the microscopic object associated with the filtered hologram pattern in the sample volume of the fluid is automatically detected on the basis of the filtered hologram pattern.

A quasi-soft catcher, a quasi-soft capture device, and a method for using the same are provided. The quasi-soft catcher includes a base and a plurality of protruding structures for capturing at least one target biological particle from a specimen. The protruding structures are regularly arranged on a board surface of the base. Each of the protruding structures includes an outer portion configured to touch the at least one target biological particle and an inner portion that is connected between the board surface and the outer portion, and a structural strength of the inner portion is less than that of the outer portion. The board surface has an interspace region arranged outside of the protruding portions, and the interspace region occupies 20-80% of the board surface.

A number analyzing method, a number analyzing device, and a storage medium for number analysis are disclosed, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device in accordance with passage of subject particles through a through-hole. Also, a particle type distribution estimating program is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.