The device (100) comprises a cavity (101) and at least two microporous membranes (102), wherein the microporous membranes (102) are arranged in series in the cavity (101) and divide the cavity (101) into a plurality of chambers (1011); each of the microporous membranes (102) is provided with micropores (103), and two adjacent chambers (1011) are in communication via the micropores (103); and each of the chambers (1011) is provided with an electrode (1012).

The objective of the present invention is to provide a particle detection device and a particle detection method that can individually and continuously detect a wide range of particles. The objective is achieved by a particle detection device including: a particle separation channel through which particles are separated according to particle sizes in a perpendicular direction to the flow of fluid; and two or more particle recovery channels that are connected to and branched from the particle separation channel, in which each of the particle recovery channels includes a particle detection unit that includes an aperture and an electric detector.

A micro-nano particle detection device and method are disclosed. The device includes a sample chamber and at least two measurement chambers, where at least one through hole is formed between each measurement chamber and the sample chamber, each measurement chamber is communicated with the sample chamber only through the through hole, a common electrode is arranged in the sample chamber, a measurement electrode is arranged in each measurement chamber respectively, a first end of the sample chamber is provided with a first liquid driving device, and the common electrode is grounded.

A particle separating device includes at least three liquid chambers adapted to store a liquid therein; at least two liquid passages, each connecting adjacent two of the liquid chambers; an inlet adapted to introduce a liquid in which multiple particles of different sizes are dispersed into one of the liquid chambers; and at least two electrodes disposed inside at least two of the liquid chambers, respectively, the electrodes adapted to apply different electrical potentials to the liquid. The cross-sectional areas of the at least two liquid passages are different from each other.

Embodiments of the microfluidic device may include of an array of microfluidic cell capture chambers, each functionalized with a different antibody to recognize a target antigen, and a network of code-multiplexed Coulter counters placed at strategic nodes across the device to quantify the fraction of cell population captured in each microfluidic chamber. For example, an apparatus may comprise a fluid inlet port divided into a plurality of separate microfluidic paths, each separate microfluidic path configured to transport a plurality of cells, the plurality of separate microfluidic paths, each comprising a plurality of microfluidic cell capture chambers, an outlet port to discharge a merged output of cells from the plurality of microfluidic cell capture chambers, and a plurality of sensors to detect cells passing into or out of a microfluidic cell capture chamber.

Systems and methods to integrate electrical sensors comprising parallel electrodes into microfluidic devices that are manufactured using soft lithography are disclosed herein. With minimal fabrication complexity, more uniform electric fields than conventional coplanar electrodes are produced. The methods disclosed are also more suitable for the construction of complex electrical sensor networks in microfluidic devices due to greater layout flexibility and provide improved sensitivity over conventional coplanar electrodes.

A particle identification sensor, including: a first flow path through which an electrolytic solution containing identifying particles can pass, the first flow path being formed in a first layer; a second flow path through which the electrolytic solution can pass, the second flow path being formed in a second layer; a through hole connecting the first flow path to the second flow path; a first electrode disposed in the first flow path; and a second electrode disposed in the second flow path, wherein the particle identification sensor identifies the identifying particles by measuring a time change of an ion current generated when the identifying particles pass through the through hole.

A microfluidic device for particle analysis, such as immunophenotyping, includes a plurality of microfluidic channels for the passage of a particle-laden fluid flow, a plurality of dedicated impedance sensors for generating impedance signals relative to each microfluidic sensor. The impedance sensors are CODES Coulter sensors, each having a distinct coded sequence for generating mutually orthogonal signals. The system uses a multi-frequency excitation signal for driving the Coulter sensors, such that the Coulter sensors generate multi-frequency impedance signals. The system outputs the multi-frequency signals of the plurality of impedance sensors as a single multi-frequency multiplexed signal, which is subsequently separated into a plurality of single-frequency multiplexed signals, which are then demodulated into single-frequency component signals corresponding to each of the Coulter sensors.

The objective of the present invention is to provide a particle detection device and a particle detection method that can individually and continuously detect a wide range of particles. The objective is achieved by a particle detection device including: a particle separation channel through which particles are separated according to particle sizes in a perpendicular direction to the flow of fluid; and two or more particle recovery channels that are connected to and branched from the particle separation channel, in which each of the particle recovery channels includes a particle detection unit that includes an aperture and an electric detector.

According to one embodiment, an analysis chip for detection of particles in a sample liquid includes a substrate, a channel provided on a surface portion of the substrate, a liquid storage portion provided on a part of the channel to store the sample liquid, holes being provided at a bottom portion of the liquid storage portion to connect the liquid storage portion and the channel, and first electrodes provided in the channel or the liquid storage portion.