A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.

Disclosed herein is a microfluidic device comprising, at least one sample inlet for receiving biological cells in a biological fluid sample; at least one sheath flow inlet for receiving a sheath fluid; at least one curvilinear channel configured to provide the biological fluid sample substantially in an outer flow and the sheath fluid in substantially an inner separated flow; a plurality of cell traps at the periphery of the curvilinear channel, each trap configured to admit a single cell having a targeted size range from the outer flow.

A compact sorting flow cytometer system is disclosed. The system includes a fluidics system having a flow cell and a deflection chamber in communication with the flow cell to receive drops in a stream of a sample biological fluid with one or more biological cells or particles and selectively deflect the drops in the stream of the sample biological fluid with the one or more biological cells or particles; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive the selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers. The DDU system includes a case or a housing with an open face surround by edges of the case, the case forming a portion of a containment chamber, the case having a top side opening aligned with the deflection chamber to receive the selectively deflected drops in the stream of the sample biological fluid into one or more containers in the containment chamber, a seal mounted around edges of the case, one or more hinges coupled to a bottom portion of the case, and a door coupled to the one or more hinges to pivot the door about the one or more hinges, the door when closed to press against the seal and close off the containment chamber from an external environment.

A method for evacuation of air in a containment chamber of a flow cytometer is disclosed. The method includes turning off a return fan in a first tunnel between an air conditioning chamber and a containment chamber; turning on an evacuation fan in a second tunnel between the air conditioning chamber and the containment chamber, the evacuation fan pulling air out of the containment chamber into the air conditioning chamber, opening a valve in an evacuation vent, the evacuation fan pushing air out of the air conditioning chamber through the evacuation vent into the environment; and continuously running the evacuation fan for a predetermined period of time to evacuate air out of the containment chamber.

A sheath flow device for an evaporation light scattering detector comprises an evaporation pipe fastener (110), an evaporation pipe heat insulating component (120), a sheath flow nozzle blocking plate (130), a sheath flow nozzle (140), a sheath flow sleeve (150), a sheath flow outlet piece (170) and a stainless steel spray needle (160). The evaporation pipe fastener, the evaporation pipe heat insulating component, the sheath flow nozzle blocking plate, the sheath flow nozzle, the sheath flow sleeve and the sheath flow outlet piece are concentrically connected orderly from front to back, and all provided with concentric inner holes. Said device is applicable to ELSD sheath flow devices ranging from nanoliter-scale to microliter-scale. On one hand, material particles entering a testing pool are enveloped and aggregated so that the formation of eddy and turbulence can be reduced, the chromatographic peak shape of a sample can be improved and the stability of sample detection can be enhanced; on the other hand, the testing pool can be cleaned so that baseline noise can be reduced and the signal to noise ratio can be increased.

A method of sorting cells including magnetically coupling a nozzle assembly to a flow cytometer in a predetermined orientation to form a fluid stream having a sheath fluid and a sample fluid. Particles within the sample fluid are oriented and then interrogated at an inspection zone to produces signals representative of emitted or reflected electromagnetic radiation. The electromagnetic radiation is analyzed to classify the cells and the cells are sorted according to their classification.

A fluid delivery method for delivering a liquid sample to a flow cell including a taper section including a first and a second inner walls opposing the first inner wall, which is inclined to the second inner wall so that a distance between the first and the second inner walls at a downstream side of the taper section is shorter than a distance at an upstream side of the taper section, and including measurement flow path provided downstream of the taper section, through which a liquid sample flows together with a sheath fluid. The fluid delivery method includes sample introduction of delivering the liquid sample into the taper section along the second inner wall until the liquid sample reaches the measurement flow path, and sample pressing by delivering the sheath fluid into the taper section along the first inner wall after the liquid sample reaches the measurement flow path.

A method for treating a biological sample, preferably a food sample which may contain one or more species of interest, including a step of decomplexification by acoustophoresis.

Provided herein is a hydrodynamic focusing device, and a related method, that enables sample flow focusing in three-dimensions for detection, isolation and sorting of target species. The device includes a multi-channel inlet and multi-channel outlet structure either side of a detection chamber. Independent control of the flow in these multiple inlets and outlets creates a sheath flow that can be controlled and steered at will.

A sensor is provided for detecting and characterizing particles in a fluid. The sensor has a microfluidic channel for receiving the fluid sample, an acoustic transducer module configured to generate a standing wave for concentrating the particles in a region of the microfluidic channel; an optical detection module configured to detect optical signals scattered by the particles upon illuminating the region of the fluid sample with a light source; and a data processing module configured to characterize the particles of the fluid sample based on the optical signals using a classifier.

A system, method, and apparatus are provided for cytometer fluidics. In one example, a pressure regulation system is provided for a cytometer fluidics system. The pressure regulation system includes a pressure regulator and a transducer. The pressure regulator pressurizes sheath fluid in a fluid path. The transducer senses a measured pressure in the fluid path independently of the sheath tank. The transducer converts the measured pressure into a voltage. The transducer communicates the voltage to the pressure regulator. The pressure regulator translates the voltage to a regulated pressure that substantially matches the measured pressure. The pressure regulator is the only pressure regulation source in the cytometry fluidics system.