A disposable injection moulded flow cell or cuvette for use in flow cytometer for in vitro assaying of human or animal whole blood and to an investigation method using the flow cytometer. The present disclosure provides a cuvette for use in an optical flow cytometer, comprising a cuboid sheath preparation area, a curved sample injection area with a rectangular cross section, a pyramidal shaped flow formation area, and a sample injector which is arranged in the transition area from the cuboid sheath preparation area to the curved sample injection area.

A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.

The present invention comprises methods and systems that use acoustic radiation pressure.

Disclosed is a dual-stage fluidics system that has reduced pulsation. This system uses inexpensive peristaltic pumps and two reservoirs that dampen pulsations in pressure. Peristaltic pumps are used as air pressure pumps and sheath fluid pumps that are placed between the two pressurized reservoirs and isolate the two reservoirs. Since the two reservoirs are maintained at a substantially even pressure, pulsations are reduced so that the peristaltic pumps essentially provide air flow and sheath fluid flow as needed.

A microchip is provided that includes a flow path through which a liquid containing a micro particle flows, an orifice through which the liquid flowing through the flow path is discharged into a space outside the microchip, and a light-irradiated portion provided at a predetermined location of the flow path and configured to be irradiated with light. A width of the flow path and a depth of the flow path at the orifice are set to be smaller than a width of the flow path and a depth of the flow path at the light-irradiated portion, and the flow path is configured to gradually decrease from upstream of the orifice in a cross-section area perpendicular to a liquid-delivering direction between the light-irradiated portion and the orifice. A cartridge including the microchip is also provided.

Apparatus and methods for sample acquisition, including for example, samples for flow cytometry systems. Certain embodiments include a plurality of plates, valves, and conduits. In particular embodiments, the plates are stacked and the conduits extend through stack of plates, and in specific embodiments each valve is in fluid communication with a conduit.

A fluid handling and delivery system useful in generating a fluid stream in the flow path of microfluidic device.

A disposable injection moulded flow cell or cuvette for use in flow cytometer for in vitro assaying of human or animal whole blood and to an investigation method using the flow cytometer. The present disclosure provides a cuvette for use in an optical flow cytometer, comprising a cuboid sheath preparation area, a curved sample injection area with a rectangular cross section, a pyramidal shaped flow formation area, and a sample injector which is arranged in the transition area from the cuboid sheath preparation area to the curved sample injection area.

A microfluidic chip orients and isolates components in a sample fluid mixture by two-step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.

To provide a technique that enables the risk of contamination to be reduced. Provided is a microparticle measuring apparatus including at least: a relay unit that enables a flow path through which microparticles flow and a cleaning fluid storage unit to communicate with each other; a sheath fluid tube that causes the flow path and a sheath fluid storage unit to communicate with each other; and a cleaning fluid tube that causes the relay unit and the cleaning fluid storage unit to communicate with each other, in which the relay unit includes a first connector that can be connected to a joint portion on the sheath fluid storage unit side of the sheath fluid tube.