This disclosure provides an impedance cytometer which includes a carrier that can be attached to a living being, with a biosensor mounted thereto. The bio sensor includes a microfluidic flow channel, formed in the carrier, and an impedance circuit. The microfluidic flow channel accommodates passage of a particle therethrough. The impedance circuit, connected to the microfluidic flow channel, includes a signal generator that produces a high-frequency drive signal applied to the flow channel to produce a biosensor output signal having high-frequency variation resulting from the drive signal and low-frequency variation resulting from impedance variation within the flow channel during the particle's passage. A lock-in amplifier is disposed to (i) amplify the bio sensor output signal, (ii) mix the amplified signal with the drive signal, and (iii) frequency-filter the mixed, amplified signal to output an impedance signal representing the low-frequency impedance variation resulting from the passage of the particle. Embodiments enable wearable, personalized cytometry.

A sensor for particle identification, the sensor comprising: a first chamber configured to be filled with an electrolytic solution; a first electrode provided inside the first chamber and configured to be connected to an external power supply for applying a voltage; a second chamber configured to be filled with the electrolytic solution; a second electrode provided inside the second chamber and configured to be connected to the external power supply; a data output means configured to output measurement data expressing an ion current generated between the first electrode and the second electrode; a partition separating the first chamber and the second chamber; and a presentation means for providing a unique identifier to an external computer device over a network. The partition includes a pore connecting the first chamber and the second chamber, a physical property of the sensor is associated with the unique identifier, the sensor is configured such that when a particle passes through the pore, a transient change dependent on at least a physical property of the pore and a physical property of the particle occurs in the ion current generated between the first electrode and the second electrode, and the unique identifier is configured to cause the external computer device receiving the unique identifier to perform a process of identifying the particle according to the physical property of the sensor associated with the unique identifier. The physical property of the sensor at least includes a physical property of the pore.

An apparatus comprising: a body having an aperture dimensioned to receive an air-borne particle of corresponding size; first and second electrodes positioned within the aperture between which a potential difference can be applied; and a measurement circuit configured to measure an electrical property between the first and second electrodes such that the presence of the air-borne particle within the aperture can be detected based on a change in the electrical property when the air-borne particle contacts both the first and second electrodes.

Provided are a device and a method for rapidly and simply detecting endotoxin without using an expensive reagent. The endotoxin detection device includes: a region containing an electrolyte solution; a partitioning member that partitions the region into two compartments such that the two compartments are in communication via a nanopore; a first electrode that is disposed in a first compartment; a second electrode that is disposed in a second compartment and is electrically connected to the first electrode; an electrolyte solution flow generating means that causes electrolyte solution in the first compartment to move to the second compartment via the nanopore; an application means that applies voltage between the first electrode and the second electrode; and a monitoring means that monitors current.

Provided are a device and a method for rapidly and simply detecting endotoxin without using an expensive reagent. The endotoxin detection device includes: a region containing an electrolyte solution; a partitioning member that partitions the region into two compartments such that the two compartments are in communication via a nanopore; a first electrode that is disposed in a first compartment; a second electrode that is disposed in a second compartment and is electrically connected to the first electrode; an electrolyte solution flow generating means that causes electrolyte solution in the first compartment to move to the second compartment via the nanopore; an application means that applies voltage between the first electrode and the second electrode; and a monitoring means that monitors current.

A method is provided for storing a particle analyzer capable of suppressing deterioration of a measurement performance with the lapse of time in a particle analyzer for analyzing particles such as exosomes, pollen, viruses, and bacteria. The particle analyzer has a first storage chamber in which a first liquid is stored, a second storage chamber in which a second liquid containing particles to be analyzed is stored, and a flow path connecting the first storage chamber in fluid communication with the second storage chamber. According to the method, at least a portion of the first storage chamber, the second storage chamber, and the flow path are surface-treated, which includes filling an internal space defined by the first storage chamber, the second storage chamber, and the flow path with a liquid to thereby store the particle analyzer in a state that the surface-treated portion is not in contact with air.

Systems and methods described herein employ focused acoustic energy applied to a reservoir containing a fluid to eject a fluid sample from the fluid sample reservoir, e.g. to an inlet of an analytical device. In many embodiments, the ejected fluid sample traverses an air gap separating the inlet of the analytical device from an upper surface of the fluid in the fluid sample reservoir. In many embodiments, the ejected fluid sample comprises one or more droplets ejected from the fluid sample reservoir, which can contain particles suspended in the fluid sample.