A number analyzing method, a number analyzing device, and a storage medium for number analysis are disclosed, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device in accordance with passage of subject particles through a through-hole. Also, a particle type distribution estimating program is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.

A method of identifying a target analyte in which a sample containing a light-emitting marker configured to bind to the target analyte is irradiated and emission from the light-emitting marker is detected. The light-emitting marker comprises a light-emitting material comprising a group of formula (I): X is one of N and B and Y is the other of N and B; Ar.sup.1 and Ar.sup.2 independently are an unsubstituted or substituted an aromatic or heteroaromatic group which is unsubstituted or substituted with one or more substituents. Ar1 and Ar2 bound to the same X group may be linked by a direct bond or a divalent group. The group of formula (I) may be a repeat unit of a light-emitting polymer. The light-emitting marker may be used in flow cytometry.

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A number analyzing method, a number analyzing device, and a storage medium for number analysis are disclosed, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device in accordance with passage of subject particles through a through-hole. Also, a particle type distribution estimating program is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.

The present disclosure provides feeder hydrogel particles that can function to support the growth, proliferation, and/or activation of a target cell in culture. The present disclosure also provides methods of culturing target cells with feeder hydrogel particles.

This application relates generally to a method and apparatus to deposit particles onto one or more coupons, and harvest particles from one or more coupons, which may beneficially provide a more uniform or localized distribution of particles over a specified area on each coupon. The application relates to a method and apparatus for depositing particles onto one or more coupons using a sieve. The application also relates to a method and apparatus for depositing particles onto one or more coupons using a dust storm. The particle loadings achieved on each coupon or across an individual coupon may be substantially uniform. The application further relates to a laser-based method and apparatus for transferring particles deposited at localized points on a source coupon to a different substrate for further use.

A calibration set includes first nanoparticles comprising nylon-6 covalently bound to a first dye, the first nanoparticles having a first average diameter and a first polydispersity index of greater than or equal to 1.15 and less than or equal to 1.19. The calibration set further includes second nanoparticles comprising nylon-6 covalently bound to a second dye that is different from the first dye, the second nanoparticles having a second average diameter and a second polydispersity index of greater than or equal to 1.15 and less than or equal to 1.19. The first average diameter is different from the second average diameter, the first average diameter and the second average diameter are each independently greater than or equal to 30 nm and less than or equal to 3000 nm, and the first average diameter is at least two times the second average diameter.

Hydrogel particles and their use in cytometric applications are described. The hydrogel particles described herein are selectively tunable to have at least one optical property substantially similar to at least one optical property of a target cell. In this regard, the hydrogel particles provided herein, in one aspect, are used as a calibration reagent for the detection of a target cell in a sample.

A method includes calibrating a cytometric device for analysis of a target cell, by inserting, into the cytometric device, a hydrogel particle. The hydrogel particle has at least one of a background fluorescent property or a spectral property that is substantially similar to the at least one of a background fluorescent property or a spectral property of the target cell. The method also includes measuring at least one property of the hydrogel particle using the cytometric device.

A method of selecting a type of particle for use in standardisation and/or calibration of a flow cytometer. The method includes determining the location of two or more particle focal points of particles flowing through a cross section of a channel in the flow cytometer; for each type of particle, determining for each particle focal point, for a beam of light directed at a type of particle at said particle focal point from a first direction, the total intensity of light scattered along a second direction; determining the difference between the highest and lowest determined light intensities of the light intensities determined at the two or more particle focal points; and selecting a type of particle for which the difference between the highest and lowest determined light intensities at the two or more particle focal points is below a predetermined threshold.

This application relates generally to a method and apparatus to deposit particles onto one or more coupons, and harvest particles from one or more coupons, which may beneficially provide a more uniform or localized distribution of particles over a specified area on each coupon. The application relates to a method and apparatus for depositing particles onto one or more coupons using a sieve. The application also relates to a method and apparatus for depositing particles onto one or more coupons using a dust storm. The particle loadings achieved on each coupon or across an individual coupon may be substantially uniform. The application further relates to a laser-based method and apparatus for transferring particles deposited at localized points on a source coupon to a different substrate for further use.