Hydrogel particles and their use in cytometric applications are described. The hydrogel particles described herein are selectively tunable to have at least one optical property substantially similar to at least one optical property of a target cell. In this regard, the hydrogel particles provided herein, in one aspect, are used as a calibration reagent for the detection of a target cell in a sample.

The present invention relates to a test sample receiving block and a microbial detection apparatus using the same. The test sample receiving block may include a sample receiving block having a sample groove portion formed therein, the sample groove portion containing the sample so that a light emission module emits light to the sample and a sensor module can detect a speckle generated when the emitted light is scattered by motion of bacteria or microbes contained in the sample, wherein the sample receiving block is surface-treated to increase an optical path of light reaching the sensor module when the emitted light is reflected or scattered by the sample accommodated in the sample groove portion, so that a pattern is formed on a surface of the sample groove portion.

Hydrogel particles and their use in cytometric applications are described. The hydrogel particles described herein are selectively tunable to have at least one optical property substantially similar to at least one optical property of a target cell. In this regard, the hydrogel particles provided herein, in one aspect, are used as a calibration reagent for the detection of a target cell in a sample.

Provided is a fine particle measuring device and the like at least including at least two light sources having different wavelength region, a detection unit that detects light from a fluorescent reference particle in accordance with excitation light from the light sources, and an information processing unit that compares, on the basis of information detected by the detection unit, a feature quantity of an output pulse based on a reference light source among the plurality of light sources with a feature quantity of an output pulse based on at least another light source among the plurality of light sources, and adjusts an output of the another light source.

Methods of calibration are provided. A method comprises introducing a material with cell-like properties and a known mass into a sensor on a measurement instrument to generate a calibration reading and adjusting an output module of the measurement instrument until the measurement instrument calibrates to the known mass for the material.

A method for determining a degree of impurity of water comprises performing (200) of a dynamic light scattering analysis of a multitude of samples of a water to be tested. Each sample of said multitude of samples comprises added single-size polymer beads of a respective size and in a respective known amount. A smallest size of the single-size polymer beads giving rise to a detectable signal, discernible over a background noise level, in a size distribution curve of the dynamic light scattering analysis is determined (220). A smallest amount of the single-size polymer of the determined smallest size giving rise to a detectable signal is determined (230). A degree of impurity of the water to be tested is assigned (240) in dependence of the determined smallest size and the determined smallest amount of the single-size polymer.

Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples.

The subject invention pertains to compositions of novel analogs of red blood cells that are distinguishable from white blood cells in a hematological instrument and processes for manufacturing such analogs. The processes for creating the compositions comprise washing, shrinking, and fixing cells at temperatures at or below room temperature.

An automated method of particle analysis is performed using an electromagnetic radiation source for generating a beam of electromagnetic radiation, a focusing element for directing the beam to a particle capture zone, a detector configured to detect a signal response from the particle capture zone and a control system. An electromagnetic radiation beam is focused onto a particle-conveying medium to define the particle capture zone for capturing a candidate particle within the beam. A first data acquisition procedure is performed to test for particle capture. If particle capture is not detected, the first data acquisition procedure is repeated. If a particle capture is detected within the beam, a second data acquisition procedure is performed to capture particle data using at least one analysis modality, following which the optical beam intensity is reduced to a sub-capture level to release the particle from the particle capture zone. The steps are repeated for successive particles in the particle-conveying medium.

The present technology provides a technique for adjusting an output difference of a light source with high accuracy in fine particle measurement for optically measuring characteristics of fine particles.

In response to this, the present technology provides a fine particle measuring device and the like at least including: at least two light sources having different wavelength regions; a detection unit configured to detect light from a fluorescent reference particle in accordance with excitation light from the light sources; and an information processing unit configured to compare, on the basis of information detected by the detection unit, a feature quantity of an output pulse based on a reference light source among the plurality of light sources with a feature quantity of an output pulse based on at least another light source among the plurality of light sources, and adjust an output of the another light source.