The present disclosure provides: reporter systems for CRISPR-mediated gene editing; gRNAs, RNPs, and compositions for the reporter systems; and methods of testing CRISPR-mediated gene editing. The present disclosure further provides compositions and methods for knocking out a DNA segment of interest; and methods of effecting CRISPR-mediated gene editing in the eye. The present disclosure also provides compositions and methods for effecting base editing using base editors.

Chimeric, engineered DNA-targeting IscB systems, methods and compositions including novel chimeric IscB polypeptides and reprogrammable targeting nucleic acid components and methods and application of use are provided.

The present disclosure relates, in general, to methods for generating engineered antigen-specific T cell receptors, cells and non-human animals comprising such engineered T cell receptors and methods of making engineered T cell receptors. The engineered T cell receptors can be specific for cancer or immunology targets, such as mesothelin, and are useful in developing therapies for cancer, autoimmune diseases, infectious diseases and other conditions or disorders.

The present disclosure relates to the field of gene genome editing. In particular, it relates to the provision of a CRISPR-Cas class 2 type V prime editing system, including a prime editor, a prime editor complex, a prime editing guide RNA system as well as the means and methods for the modification of a nucleic acid sequence of interest with the prime editing system of the present invention.

Herbicide-resistant genes, polypeptides and their applications in plant breeding are provided. Specifically, the disclosure provides an application of an HIR1 protein in the preparation of a herbicide-resistant plant. After the HIR1 protein of a plant is overexpressed, the plant has strong resistance to herbicide, and has very broad application prospects in improving and cultivating the herbicide-resistant plant.

Systems, methods, and kits for separating CRISPR/Cas components are provided. The systems, methods, and kits utilize ion exchange chromatography to separate CRISPR/Cas components based on the differing charges of CRISPR-Cas components.

Provided herein are methods of regulating alternative polyadenylation (APA) of a target RNA in a cell, increasing stability of a target RNA in a cell, preventing degradation of a target RNA in a cell, preventing degradation of a target RNA in a cell, modifying localization of a target RNA in a cell, or increasing synthesis of a protein encoded by a target RNA in a cell, by administering to the cell an RNA regulation unit, wherein the RNA regulation unit comprises an RNA binding protein (RBP) and a gene-targeting agent, wherein the RNA binding protein binds proximal to a poly(A) signal and/or site.

Disclosed herein is a method for identifying a subject with increased risk of developing a cardiometabolic disease or a hematological cancer, that involves the steps of (a) assaying a blood, serum, or plasma sample from the subject for circulating levels of myeloid zinc finger 1 (MZF1), anti-mllerian hormone (AMH), TIMP metallopeptidase inhibitor 1 (TIMP1), glycine N-methyltransferase (GNMT), or a combination thereof; and (b) comparing the circulating levels of MZF1, AMH, TIMP1, GNMT, or a combination thereof, to control values, wherein presence of elevated circulating levels of MZF1, AMH, TIMP1, GNMT, or a combination thereof indicates an increased risk of developing a cardiometabolic disease and/or a hematological cancer.

Disclosed are compounds and methods for changing a DNA sequence of an organism comprising delivering to the organism a change agent material which includes a strand invasion agent and a comparison agent. The strand invasion agent is configured to cause a separation of a double-stranded DNA strand in an organism cell. The comparison agent is configured to bind to a first single-stranded organism DNA strand, by including material that presents a number and configuration of hydrogen bonds complementary to the first single-stranded organism DNA strand, except, intentionally, at one or more base pair locations. Accordingly, when the comparison agent material binds to the first single-stranded organism DNA strand and at least one mismatched base pair is indicated on the first single-stranded organism DNA strand as a result of the binding, the organism initiates a mismatch repair process.

Provided herein are methods of analyzing DNA comprising degrading forms of DNA sequences that are prevalent in healthy subjects; and detecting sequences that are not degraded. Some such methods facilitate detection of aberrant forms of DNA.