Embodiments disclosed are systems and methods for interfacing a metallic capillary in a microchannel of a metallic body. A method may include inserting a portion of the metallic capillary into a portion the microchannel of the metallic body, sintering the portion of the metallic capillary to the portion of the microchannel of the metallic body, disposing a sacrificial powder at least proximate to the metallic capillary and the metallic body after sintering the portion of the metallic capillary and the portion of the microchannel of the metallic body, and infiltrating at least the portion of the metallic capillary sintered to the portion of the microchannel of the metallic body with an infiltrant in the presence of the sacrificial powder disposed at least proximate to the metallic capillary and the metallic body.

This disclosure describes techniques for fabricating a high-resolution, non-cytotoxic and transparent microfluidic device. A material can be selected based on having an optical property with a predetermined degree of transparency to provide viewability of a biological sample through the microfluidic device and a level of cytotoxicity within a predetermined threshold to provide viability of the biological sample within the microfluidic device. An additive manufacturing technique can be selected from a plurality of additive manufacturing techniques for fabricating the microfluidic device based on the selected material to provide a resolution of dimensions of one or more channels of the microfluidic device higher than a predetermined resolution threshold.

Providing a microchip that effectively suppresses the peeling of a bonded substrate that has been bonded together and that is unlikely to leak liquid. A microchip includes: a bonded substrate that includes a plurality of substrates, each of the substrates having a main surface and a side surface, the main surfaces being bonded each other; and a microchannel located inside the bonded substrate. The substrates include a first substrate with a large thickness and a remaining substrate with a smaller thickness than the first substrate. At least the first substrate has a side surface having a gradient and extending to an outermost side of the bonded substrate when one end of the bonded substrate is viewed from a direction parallel to the main surface.

Techniques regarding microfluidic chips with one or more vias filled with sacrificial plugs and/or manufacturing methods thereof are provided herein. For example, one or more embodiments described herein can comprise an apparatus, which can comprise a silicon device layer of a microfluidic chip comprising a plurality of vias extending through the silicon device layer. The plurality of vias comprise greater than or equal to about 100 vias per square centimeter of a surface of the silicon device layer and less than or equal to about 100,000 vias per square centimeter of the surface of the silicon device layer. Additionally, the apparatus can comprise a plurality of sacrificial plugs positioned in the plurality of vias.

A fluid line part for a microfluidic device includes a first substrate having a first surface in which at least one depression is provided, the depression forming a channel for conducting a fluid along a main flow direction. At least one support web extends lengthwise inside the channel along the main flow direction. The support web is configured and positioned such that fluid flows freely around it.

The present invention relates to a method for producing a microfluidic device, in particular, a sol-gel method for producing a microfluidic device in hybrid silica glass. The invention also relates to a microfluidic device obtainable by the method as described above and to microfluidic device in hybrid silica glass comprising at least one microchannel having a depth of at least 1 μm, preferably between 1 μm and 1 mm, and more preferably between 10 and 100 μm.

A microchip includes: a first substrate; a second substrate partially bonded to the first substrate, the second substrate having a main surface and an outer side face; a hollow channel located between the first substrate and the second substrate, the channel extending in a direction along the main surface of the second substrate; a liquid distribution port formed to penetrate the second substrate; a first bonding section that bonds the first substrate to the second substrate to surround the channel when viewed from a direction orthogonal to the main surface; a second bonding section located at a position closer to the outer side face of the second substrate than the first bonding section, and that bonds the first substrate to the second substrate; and an internal space provided between the first substrate and the second substrate, and that communicates with a space outside the first substrate and the second substrate.

The systems and methods of the present disclosure provide techniques for the design and use of an intermediate or transitional plate or block designed to couple acoustic energy at a given frequency from a transducer, such as a piezoelectric transducer, to one or more acoustophoretic devices, such as microfluidic channels, such that driving the chip occurs with a controlled wavelength and symmetry. Such techniques provide improved efficiency when driving a single acoustophoretic device, or for multiple acoustophoretic devices to be operated in concert from a single transducer, and therefore without complex electronics. Additionally, the techniques described herein allow for relaxed design constraints when considering transducer selection and fabrication, instead transferring design constraints to the more easily customized actuation plate.

Provided are integrated analysis devices having features of macroscale and nanoscale dimensions, and devices that have reduced background signals and that reduce quenching of fluorophores disposed within the devices. Related methods of manufacturing these devices and of using these devices are also provided.

This disclosure describes techniques for fabricating a high-resolution, non-cytotoxic and transparent microfluidic device. A material can be selected based on having an optical property with a predetermined degree of transparency to provide viewability of a biological sample through the microfluidic device and a level of cytotoxicity within a predetermined threshold to provide viability of the biological sample within the microfluidic device. An additive manufacturing technique can be selected from a plurality of additive manufacturing techniques for fabricating the microfluidic device based on the selected material to provide a resolution of dimensions of one or more channels of the microfluidic device higher than a predetermined resolution threshold.