The present invention relates to the field of upgrading biomass, in particular algal biomass, and more specifically the present invention relates to a method for extracting water-soluble compounds from microalgae and/or cyanobacteria, as well as the product obtained by this method and the uses of same, in particular in the food industry or as food supplements.

This disclosure relates to techniques and methods to isolate and purify cannabinoids, such as CBDV, CBD, CBC, THCV, THC, CBN, CBG, CBDA, THCA, or CBGA. Evaporation and sonicating techniques are used to isolate and purify cannabinoids, such as CBDV, CBD, CBC, THCV, THC, CBN, CBG, CBDA, THCA, or CBGA. The resulting compounds find further use within the devices and compositions described herein as well as for preparative and analytical methods.

The disclosure discloses an ultrasonic-assisted pretreatment method for extraction of multiple steroid hormones in a sediment, including the following steps: (1) lyophilizing the sediment, grinding the sediment, and passing the ground sediment through a 40-60-mesh sieve; (2) placing the sample obtained in step (1) in a container; (3) adding an extractant to the container in step (2), shaking the mixture for 15 s-30 s, centrifuging the mixture to collect an supernatant after ultrasonication, and repeating extraction three times; where the extractants used in the three times of extraction are two of methanol, acetonitrile and acetone; and (4) after mixing the supernatants of the three times of extraction obtained in step (3), concentrating the mixture under a nitrogen flow at 20-30° C., passing the concentrated mixture through a filter, and performing detection. By using the method of the disclosure, the maximum recovery can be up to 100%. The application range is wide, and a recovery of the plurality of steroid hormones each can be up to 73% or above.

An extraction method for preparing samples for analytical analysis is disclosed. The method includes the steps of placing a sample matrix containing one or more analytes in a heat conductive sample cup, positioning the heat conductive sample cup in a pressure-resistant reaction chamber, dispensing solvent into the heat conductive sample cup, dispersing the solvent and the sample matrix in the sample cup in the reaction chamber, heating the sample matrix and the extraction solvent in the heat conductive sample cup in the reaction chamber to a temperature at which the dispensed solvent generates an above-atmospheric pressure, and releasing the extraction solvent extract from the sample cup at atmospheric pressure.

The present invention relates to the use of coconut water as extraction solvent, to extraction methods using coconut water and extracts obtained by extraction with coconut water.

A method for extracting compounds, including stable natural cannabinoid acids, from plant material, utilizing terpenes as a saturant is described. The invention includes the excitation of the plant material and terpene solvent with microwave, ultrasound, sonication, heat input, and physical agitation or combinations thereof. The invention particularly covers the process as it relates to the extraction of THC-A, CBG-A, and CBD-A and their derivatives from cannabis and hemp for the use in products for medical and recreational use. The combinations of terpene saturant, plant material strain and process variables can be tuned in order to dial in the final resultant product for several variables including but not limited to terpene content, THC-A, CBG-A or CBD-A potency, ratios of THC-A, CBD-A, CBG-A and their derivatives, or flavor profile.

A method for extracting compounds from plant material utilizing terpenes as a solvent is described. The invention includes the excitation of the plant material and terpene solvent with microwave, ultrasound, heat input, and physical agitation or combinations thereof. The invention particularly covers the process as it relates to the extraction of THC and CBD and their derivatives from cannabis and hemp for the use in products for medical and recreational use. The combinations of terpene saturant, plant material strain and process variables can be tuned in order to dial in the final resultant product for several variables including but not limited to terpene content, THC or CBD potency, ratios of THC or CBD and their derivatives, or flavor profile.

A CO.sub.2 extraction process for cannabis sativa that uses liquid CO.sub.2 in combination with co-solvent admixtures to purify cannabis botanicals in high yield and purity. The extraction process allows for multiple extractions, or washes, to be performed with the same solvent CO.sub.2, which may be seamlessly recycled and purified between subsequent extraction cycles. A variety of in-line filtration vessels, pumps, vacuums, and controllable valves are used to yield a pure product while allowing a high level of user control over the process.

Solvent-based methods for the extraction and concentration of cannabinoids and other target compounds from large-scale harvests of cannabis and hemp crops. Select portions of cannabis or hemp plants are harvested, ground to a desired particle size and ultimately powderized where the powder is mixed with a solvent, preferably ethanol or water, to form a slurry that is subjected to at least two, and preferably five or more separate inverting filter centrifugation steps until cannabinoid extraction is maximized. The filtrate from the first inverting centrifugation step is isolated and purified to derive an extract whereas the powderized filtride is subjected to further centrifugation steps. Fresh solvent is introduced at the last inverting centrifugation step and subsequently used upstream in sequentially-earlier centrifugation steps until ultimately utilized in a first inverting filter centrifugation step.

Some variations provide a system for extracting a product from biomass, comprising: a process chamber having an internal volume; one or more mechanical elements configured to controllably and reversibly mechanically seal the process chamber and reduce the internal volume to mechanically compress the biomass; a fluid port in flow communication with the process chamber; and a collection sub-system in flow communication with the fluid port. Other variations provide a method of extracting a product from biomass, the method comprising: introducing biomass into a process chamber; mechanically sealing the process chamber; mechanically compressing the biomass to release a first fluid material; mechanically decompressing the biomass; introducing an extraction solvent into the process chamber; maintaining process-chamber pressure from about 1 bar to about 1000 bar, wherein the extraction solvent extracts a second fluid material; and recovering the second fluid material from the process chamber. High processing throughput is enabled with this invention.