The present invention describes a water filter apparatus (100, 100a) with integrated nutrient enrichment and/or enhancement. Desired nutrients, ions, hydrogen ions, ceramic, stones, metal etc. packed in granules or balls are selectively disposed inside a stick capsule (160), shell capsule (180) or hydrogen capsule (190). One or two stick capsules (160) are located inside a filter element (150). The stick capsule has a tapered end (162) and in association with T-shaped posts (157) formed on an inside of a cap (151) of the filter element, dynamic flow of water along the tapered end (162) induces nutrient-rich water to flow through perforations (165) at the end of the stick capsule (160), mixes/blends the two streams of water before water is discharged through a faucet (144). The filtered water is also enhanced by treating the water through flavor treatment, anti-bacterial, anti-chlorine, activated carbon and so on.

Disclosed herein is a method for obtaining compounds and compositions from plant and fungus materials by thermal treatment, affinity capture, filtration, and release through multi-phasic transitions between gas, solid, and liquid states. The compounds of interest are obtained by manipulating the temperature and pressure of the heating chamber. The compounds in gas phase are passed through an affinity medium which captures the compounds of interest in either solid or liquid phase by exposing the compound of interest to the localized micro-affinity environment of the medium. The compounds are separated from the medium using direct competition with solvent or buffers optimized for the specific chemical properties of compounds.

Disclosed herein is a method for obtaining compounds and compositions from plant and fungus materials by thermal treatment, affinity capture, filtration, and release through multi-phasic transitions between gas, solid, and liquid states. The compounds of interest are obtained by manipulating the temperature and pressure of the heating chamber. The compounds in gas phase are passed through an affinity medium which captures the compounds of interest in either solid or liquid phase by exposing the compound of interest to the localized micro-affinity environment of the medium. The compounds are separated from the medium using direct competition with solvent or buffers optimized for the specific chemical properties of compounds.

The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.

A method of determining total organofluorine in a sample comprising PFAS, comprising: providing a solution of PFAS in an organic solvent to obtain extracted PFAS, or extracting a sample with an organic solvent to obtain extracted PFAS; treating the extracted PFAS with a sodium metal dispersion and alcohol to obtain sodium fluoride; and quantifying the amount of the fluoride. Surprisingly, we discovered that the method recovered substantial fluorine from PFAS and significantly higher yields obtained by selection of the appropriate alcohols. The method is selective for organofluorine from inorganic fluorine.

In one embodiment, a system of PFAS (Per-Poly-fluorinated alkyl substances) effluent liquid treatment includes: a pump to pump a liquid received via an intake; a sediment filter to filter sediment from the liquid; a granular activated carbon (GAC) device, disposed downstream of the sediment filter, to remove organic contaminants from the liquid; one (preferably two) or more ion exchange resin columns, disposed downstream of the GAC device, to remove PFAS constituents from the liquid; and a plurality of control valves being controlled to direct the liquid to flow along one or more liquid flow paths through the ion exchange resin columns so as to adjust a rate of processing the liquid by the one or more ion exchange resin columns. The system is disposed in a shipping container to be transported to a destination and is set up in the container onsite at the destination.

Certain configurations of devices and systems which are configured to draw a selected volume of an air sample into a trap are described. In some examples, the devices and systems comprise a pump and a mass flow sensor to draw a selected volume of the air sample through a trap even where variable restriction occurs.

Certain configurations of devices and systems which are configured to draw a selected volume of an air sample into a trap are described. In some examples, the devices and systems comprise a pump and a mass flow sensor to draw a selected volume of the air sample through a trap even where variable restriction occurs.

A significant reduction in extra-column band broadening can be achieved by decoupling the injection system from the main solvent flow line. In addition, by decoupling the injection system from the main solvent flow line, additional components (e.g., filters, valves, etc.) can be introduced into the chromatography system without increasing the negative band broadening effects. Systems and methods herein provide not only for such decoupling but also for filtering precipitates from the system. As a result, a larger volume of sample can be injected into the present systems without compromising separation yield. In addition, an increase the column loading per batch, an increase the overall yield of separations, and greater system efficiency (i.e., less time lost for cleaning and maintenance) can be realized.

The invention discloses a method and apparatus for cell cultivation, comprising a bioreactor, an acoustic standing wave cell separator and a filter, wherein an outlet of the bioreactor is fluidically connected to an inlet of the acoustic standing wave cell separator and a media outlet of the acoustic standing wave cell separator is fluidically connected to the filter.