A method of preparing a liquid mixture for use in a liquid chromatography system is provided. The mixture comprises one or more acids, one or more bases, one or more solvents and water, and the method comprises the steps of: calculating pH and/or solvent concentration at a particular time t from a user-determined gradient function; and, based on the values obtained, calculating percent acid, percent base, percent solvent and percent water in the liquid mixture at time t. A liquid chromatography system incorporating such method is also provided.

A sample injector configured to introduce a sample fluid into a mobile phase, wherein the mobile phase is to be driven by a mobile phase drive through a separation unit for separating compounds of the sample fluid in the mobile phase, wherein the sample injector comprises a metering device being operable for displacing fluid and for intaking a metered amount of the sample fluid into the sample injector, an injector valve being switchable for operating the sample injector selectively in a sample intake mode in which the metering device is operable to intake the sample fluid from a sample container, or a separation mode in which intaken sample fluid is driven between the mobile phase drive and the separation unit for separating the compounds, and a flow direction controller configured for defining an enabled flow direction of fluid displaced by the metering device and for defining a disabled flow direction.

There is provided a system for performing a chromatographic separation of an analyte, methods of using the system to separate at least one component of an analyte and an eluent generator of use in the system. An exemplary system comprises: (a) an eluent generator comprising: (i) a housing configured to be pressurizable by gas, comprising an annular void defined by the housing, and a gas inlet for the gas and a gas outlet for the gas in fluid communication with the annular void; (ii) a membrane permeable to the gas defining an eluent flow channel disposed within the annular void, the eluent flow channel having an eluent precursor fluid inlet and an eluent outlet; (iii) a source of gas in fluidic communication with the gas inlet; (iv) a source of the eluent precursor fluid; and (b) a chromatography column disposed downstream of and in fluidic communication with the eluent outlet.

A channel bubble reduction device includes a liquid accommodation portion, that accommodates a liquid, a liquid supply apparatus that, with a pushing operation of a rod, discharges the liquid through an aperture portion of a tube portion, a first channel that connects the aperture portion of the liquid supply apparatus with the liquid accommodation portion, and an air layer formation apparatus that forms an air layer in at least one of the first channel or the tube portion.

A channel bubble reduction device includes a liquid accommodation portion, that accommodates a liquid, a liquid supply apparatus that, with a pushing operation of a rod, discharges the liquid through an aperture portion of a tube portion, a first channel that connects the aperture portion of the liquid supply apparatus with the liquid accommodation portion, and an air layer formation apparatus that forms an air layer in at least one of the first channel or the tube portion.

Tailored chromatographic media and methods for using the tailored chromatographic media to purify mixtures extracted from cannabis to obtain a cannabinoid having greater than about 90% purity. In an embodiment, the tailored chromatographic media may comprise a porous resin and/or porous carbon and have a surface area of greater than about 900 m2/g, wherein the tailored chromatographic media may further comprise micropores, mesopores, macropores, wherein the tailored chromatographic media may further comprise at least two distributions of macroporous pore sizes, wherein the at least two distributions of macroporous pore sizes may comprise a first population having a macroporous pore size denoted x and a second population having a macroporous pore size denoted y, wherein a ratio of x/y may be about 1:1, and wherein the tailored chromatographic media may further comprise an anionic polysaccharide and a functional moiety.

The present invention provides methods for isolating an active polypeptide or immunoconjugate by purification of a solution containing both the active polypeptide or immunoconjugate and an acidic variant thereof, such as a deamidated variant, using anion exchange chromatography. The present invention also provides compositions, formulations, and unit dosage forms comprising the purified polypeptide or immunoconjugate.

A target component is collected using a preparative separation and purification device having a holder for holding a trap column in which the target component has been captured, a liquid feeder for feeding a first solvent having compatibility with the water remaining in the trap column and a second solvent having low compatibility with water and high compatibility with the first solvent into the trap column, a flow-path switch for connecting the exit end of the trap column to a waste liquid flow path and a collection flow path, and a control unit for controlling the flow-path switch so that solution including water flows into the waste liquid flow path.

A sample separation apparatus includes a first-dimension separation unit for separating the fluidic sample, having a first-dimension outlet for outputting the fluidic sample or fractions thereof, and a second-dimension separation unit for further separating the fluidic sample or fractions thereof. The second-dimension separation unit has a second-dimension inlet fluidically coupled to the first-dimension outlet. A modulation unit, coupled between the first-dimension outlet and the second-dimension inlet at a first coupling point, is configured for withdrawing fluid from the first coupling point and for ejecting fluid into the first coupling point. A second-dimension fluid drive is coupled to a second coupling point located between the first-dimension outlet and the second-dimension inlet and downstream from the first coupling point. The second-dimension fluid drive is configured for generating a fluid flow for driving at least part of the fluidic sample after treatment by the first-dimension separation unit through the second-dimension separation unit.

A thermal impact assembly for a sample separation apparatus for separating a fluidic sample in a mobile phase by a sample separation unit includes a thermal impact device configured for thermally impacting the fluidic sample and/or the mobile phase and the sample separation unit, and a control unit configured for controlling the thermal impact device for thermally impacting the fluidic sample and/or the mobile phase on the one hand and for thermally impacting the sample separation unit on the other hand independently from each other.