The present invention relates to a polymer including at least one structure selected from the group consisting of a structure represented by General Formula (3) described below and a structure represented by General Formula (4) described below:

##STR00001## in General Formula (3) and General Formula (4) described above, X.sub.31 and X.sub.41 represent a hydrophilic group-containing structure, n represents an integer of 0 to 2, R represents a hydrogen atom or an alkyl group, Y.sub.31 to Y.sub.32 and Y.sub.41 to Y.sub.43 each independently represent a hydrophilic group-containing structure, a hydrogen atom, or an alkyl group.

The present invention relates to a polymer including at least one structure selected from the group consisting of a structure represented by General Formula (3) described below and a structure represented by General Formula (4) described below:

##STR00001## in General Formula (3) and General Formula (4) described above, X.sub.31 and X.sub.41 represent a hydrophilic group-containing structure, n represents an integer of 0 to 2, R represents a hydrogen atom or an alkyl group, Y.sub.31 to Y.sub.32 and Y.sub.41 to Y.sub.43 each independently represent a hydrophilic group-containing structure, a hydrogen atom, or an alkyl group.

A method of operating a chromatography column is described. This method involves collecting column outlet signal and accumulated flow parameters at two or more intervals of at least one mobile phase transition front during operation of the chromatography column comprising column packing. A model gamma cumulative distribution curve is calculated based on the collected column outlet signal and accumulated flow parameters for the at least one mobile phase transition front. A height equivalent theoretical plate (HETP) value is calculated for the at least one mobile phase transition front using parameters of the model gamma cumulative distribution curve and the quality of the chromatography column packing is assessed based on the calculated HETP value.

A method of operating a chromatography column is described. This method involves collecting column outlet signal and accumulated flow parameters at two or more intervals of at least one mobile phase transition front during operation of the chromatography column comprising column packing. A model gamma cumulative distribution curve is calculated based on the collected column outlet signal and accumulated flow parameters for the at least one mobile phase transition front. A height equivalent theoretical plate (HETP) value is calculated for the at least one mobile phase transition front using parameters of the model gamma cumulative distribution curve and the quality of the chromatography column packing is assessed based on the calculated HETP value.

The present disclosure relates to separation and purification methods for a vaccine virus using affinity chromatography, and more particularly, to a purification method for a virus capable of obtaining a vaccine virus with a high purity and a high yield using affinity chromatography containing a vaccine virus-affinity resin.

The patent discloses a method for efficiently collecting and purifying Orobanche cumana (O. cumana) germination stimulants using aeroponic system and solid-phase extraction (SPE), including the following steps: (1) sunflower seeds germination, then planting sunflower seedlings in aeroponic device, and cultivating the sunflower seedlings in the aeroponic system; at the aeroponic stage, phosphorus-containing aeroponic nutrient solution is first used to cultivate the sunflower seedlings for 20 to 25 days, and phosphorus-free aeroponic nutrient solution is then used instead to subject the sunflower seedlings to starvation cultivation for 5 to 7 days; and (2) passing all nutrient solutions in the aeroponic device through an SPE cartridge for SPE to extract O. cumana germination stimulants. The obtained O. cumana germination stimulants are diversified, and have high concentration and purity.

The patent discloses a method for efficiently collecting and purifying Orobanche cumana (O. cumana) germination stimulants using aeroponic system and solid-phase extraction (SPE), including the following steps: (1) sunflower seeds germination, then planting sunflower seedlings in aeroponic device, and cultivating the sunflower seedlings in the aeroponic system; at the aeroponic stage, phosphorus-containing aeroponic nutrient solution is first used to cultivate the sunflower seedlings for 20 to 25 days, and phosphorus-free aeroponic nutrient solution is then used instead to subject the sunflower seedlings to starvation cultivation for 5 to 7 days; and (2) passing all nutrient solutions in the aeroponic device through an SPE cartridge for SPE to extract O. cumana germination stimulants. The obtained O. cumana germination stimulants are diversified, and have high concentration and purity.

A preparative closed cycle supercritical fluid column chromatography system, device, and method of isolating high volumes of pure components from mixtures using a supercritical solvent. Bulk fractions of desirable material from plants can be obtained using supercritical fluid column chromatography with a chromatography column. A chemical sensor downstream the chromatography column detects chemical species eluted from the column and a plurality of collection columns collects the bulk fractions of product with a control system controlling the collection valves based on detection of chemical species at the chemical sensor.

Functionalised polymeric chromatography medium, comprising: at least one non-woven sheet comprising one or more polymeric nanofibers having a mean diameter of 10-1000 nm; one or more polymer chains grafted onto the one or more polymeric nanofibers, wherein the polymer chains are poly-glycerol chains comprising glycidol monomer residues or wherein the polymer chains comprise divinylsulfone monomer residues; and at least one ligand group bonded to the one or more polymer chains.

Functionalised polymeric chromatography medium, comprising: at least one non-woven sheet comprising one or more polymeric nanofibers having a mean diameter of 10-1000 nm; one or more polymer chains grafted onto the one or more polymeric nanofibers, wherein the polymer chains are poly-glycerol chains comprising glycidol monomer residues or wherein the polymer chains comprise divinylsulfone monomer residues; and at least one ligand group bonded to the one or more polymer chains.