A nucleic acid collection column collects a nucleic acid from a liquid sample containing the nucleic acid. The nucleic acid collection column includes a sample injection portion having an opening into which the liquid sample containing the nucleic acid is injected, a support adsorption portion that houses a support for adsorbing the nucleic acid and in which the nucleic acid is adsorbed on the support, and a discharge portion that discharges a liquid passed through the support adsorption portion. The support includes aluminum oxide having a surface where a water-soluble neutral polymer is adsorbed. A space that houses the support in the support adsorption portion has a cylindrical shape, and has a volume of 0.13 μL or more and 13.5 μL or less. An aspect ratio (d.sub.1/d.sub.2) of the space satisfies 1.0≤d.sub.1/d.sub.2<15.0.

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are chromatographic materials comprising having a narrow particle size distribution.

A preparative liquid chromatograph includes a liquid chromatograph section, a trap section, an eluent supply section, a collector, and a flow path switching section. The flow path switching section is configured to be selectively switched to a component trap mode that connects the liquid chromatograph section and the trap section in such a way that a sample component separated in a separation column is trapped by a trap column of the trap section; and a collection mode that connects the eluent supply section and the trap section and connects the trap section and the collector in such a way that the components trapped in the trap column are eluted by an eluent from the eluent supply section and are guided to the collector.

A preparative liquid chromatograph includes a liquid chromatograph section, a trap section, an eluent supply section, a collector, and a flow path switching section. The flow path switching section is configured to be selectively switched to a component trap mode that connects the liquid chromatograph section and the trap section in such a way that a sample component separated in a separation column is trapped by a trap column of the trap section; and a collection mode that connects the eluent supply section and the trap section and connects the trap section and the collector in such a way that the components trapped in the trap column are eluted by an eluent from the eluent supply section and are guided to the collector.

A method for separating full Adeno-associated virus (AAV) capsids from empty AAV capsids in a buffered mixture comprising full AAV capsids, empty AAV capsids, comprising the steps of contacting the buffered mixture with a first substrate bearing a metal affinity ligand attached to the first substrate, said metal affinity ligand having the ability to complex metal ions via three or more nitrogen atoms, separating empty AAV capsids from full AAV capsids by eluting with a pH gradient, a salt gradient, a metal ion gradient or a combination thereof in the presence of multivalent cations bound to the metal affinity ligand to obtain a purified full AAV capsid fraction.

For removing contaminating DNA in the mixture or purified AAV capsid fraction, the method of the invention can be combined with contacting of the buffered mixture or the purified full AAV capsid fraction with a second substrate bearing a metal affinity ligand attached to the second substrate in the presence of multivalent cations bound to the metal affinity ligand, said metal affinity ligand comprises two or more negatively charged carboxylic acid residues.

[Problems] To provide a method for treating hydroxyapatite filler so that it can be used multiple times in the separation of a charged material included in a sample liquid using adsorbent composed of the hydroxyapatite filler, a production method including the treatment method, and hydroxyapatite filler.

[Means to solve problems] The treatment method of the present invention comprises a first step of bringing a first liquid containing a predetermined material into contact with hydroxyapatite filler, and a second step of bringing a second liquid containing an alcohol into contact with the hydroxyapatite filler.

The present disclosure provides a porous silica having an average pore diameter of at least 210 Å and a pore volume of at least 0.80 cm.sup.3g.sup.−1. The present disclosure also provides a method of producing the porous silica including gelling a liquid phase-dispersed nanoparticulate silica in the presence of either (i) a Brønsted acid and an amine group having two or more primary or secondary amine groups or (ii) an amino acid.

The invention relates to the isolation or extraction of exosomes.

The invention relates to the isolation or extraction of exosomes.

Provided herein are, inter alia, biological manufacturing and downstream purification processes.