The invention relates to a method for manufacturing a multicapillary packing for an exchange of material including the formation, by a 3D printing method, of a monolith having a porous mass through which a plurality of parallel channels passes, opening on an inlet face and an outlet face of the packing, the 3D printing method being chosen among: selective laser sintering, molten wire deposition, stereolithography, binder spraying and spraying of material, the porous mass being suitable for allowing the diffusion of material to be exchanged between the channels.

The invention concerns a method for producing a chromatography analysis column, the resulting column, and a device comprising such a column. The method according to the invention comprises the following steps: (a) depositing on the flat surface of a substrate a first layer of particles which are intended to form the stationary phase; (b) depositing on the layer at least one second layer of compactly assembled particles; (c) impregnating the first and second layers with a light radiation-sensitive material, to form at least two compactly assembled particle layers impregnated with sensitive material; (d) insolating these layers in the regions corresponding to the desired internal shape of the chromatography analysis column, if the light radiation-sensitive material behaves like a positive resin, or outlining this internal shape if the light radiation-sensitive material behaves like a negative photosensitive resin; (e) eliminating either the regions insolated in step (d) if the light radiation-sensitive layer behaves like a positive photosensitive resin, or the regions not insolated in step (d) if the light radiation-sensitive material behaves like a negative photosensitive resin; and (f) covering and sealing the structure obtained in step (e) with a cover covered on the face facing the layers with at least one layer of compactly assembled particles which are identical to or different from those deposited on the substrate surface. The invention is used in particular in the field of chemical analysis.

An apparatus for suppressing an eluent of an aqueous sample stream including analyte ions of one charge, positive or negative, comprises a primary channel member, a first block, a first regenerant flow channel, a first charged barrier, a second block, a second regenerant flow channel, a second charged barrier, a first stationary flow-through ion exchange material, and optionally a first electrode and a second electrode. The first stationary flow-through ion exchange material comprises a polyolefin substrate having a functional polymer layer disposed thereon. The polyolefin substrate has a pore structure with a pore size ranging from about 5 microns to about 250 microns. The functional polymer layer has a thickness ranging from about 1 micron to about 20 microns, and a layer pore structure having a pore size ranging from about 1 nm to about 100 nm. The functional polymer layer comprises an ion exchange layer.

A solid phase for use in separation has been modified using an aqueous phase adsorption of a headgroup-modified lipid to generate analyte specific surfaces for use as a stationary phase in separations such as high performance liquid chromatography (HPLC) or solid phase extraction (SPE). The aliphatic moiety of the lipid adsorbs strongly to a hydrophobic solid surface, with the hydrophilic and active headgroups orienting themselves toward the more polar mobile phase, thus allowing for interactions with the desired solutes. The surface modification approach is generally applicable to a diversity of selective immobilization applications such as protein immobilization clinical diagnostics and preparative scale HPLC as demonstrated on capillary-channeled fibers, though the general methodology could be implemented on any hydrophobic solid support material.

The present invention relates to a preparative chromatography system (200, 500, 800) and a chromatography process (400, 700) adapted to repetitive cycling of chromatography volumes. The system (200, 500, 800) comprises at least two upstream pumps (203a, 803a, 203b, 803b) and separate flow paths (220) from process liquid sources to the chromatography device (200, 500, 800). The system (200, 500, 800) is arranged to prime one flow path (220) with one process liquid while providing another process liquid to the chromatography device and thereby minimizing the hold-up volume of the system (200, 500, 800).

The present invention relates to a chromatography column (100; 100′) comprising a tubular side wall (110) having an inner wall (112), an adaptor assembly (120) and a base assembly (125). An enclosed bed space (130) is defined between said adaptor assembly (120), said base assembly (125) and said inner wall (112) of said tubular side wall (110). The adaptor assembly (120) is axially movable inside said tubular side wall (110) in relation to said base assembly (125). The inner wall (112) comprises at least one groove (180; 180′; 180″; 180′″) which is positioned in a lower half of the tubular side wall (110) of the chromatography column (100), via which groove (180; 180′; 180″; 180′″) air and other fluid can pass the adaptor assembly (120) when the adaptor assembly is positioned in a priming position, in which priming position said adaptor assembly intersects with at least a part of the at least one groove (180; 180′; 180″; 180′″).

An object of the present invention is to suppress the variation of the elution position of a compound having a charged portion by a preservation liquid, in the purification of the compound, without carrying out the substitution step of the preservation liquid attached to the adsorbent used for the purification and the keeping step. A method for purifying a compound having a charged portion, the method comprising the steps of: preparing a composition containing a compound having a charged portion; preparing a buffer solution comprising a buffering agent and an alcohol, the buffer containing a calcium phosphate compound at least partially, having a buffer capacity in a range of pH 6.0 to pH 8.0, and being soluble in a polar solvent and insoluble in a non-polar solvent; preserving an adsorbent in the buffer solution; adsorbing the compound on the adsorbent by bringing the composition into contact with the adsorbent preserved in the buffer solution; and separating the compound from the adsorbent by gradient elution.

There are provided porous fibers having excellent removal performance with respect to a material to be purified; and a purification column into which an adsorbent material obtained by bundling the fibers is incorporated. The porous fibers satisfying the following conditions (a) and (b) and having a shape in which three or more projected parts are continuously present in the lengthwise direction on the periphery part of a solid-state fiber: (a) The modification degree Do/Di in a cross section is 1.2 to 6.6 when the diameter of the inscribed circle is denoted by Di and the diameter of the circumscribed circle is denoted by Do., and (b) The specific surface area of pores is 50 m.sup.2/g or more.

A chromatographic cassette includes a cassette including a chamber, chromatographic media disposed within the cassette chamber, a distribution network fluidly coupled to the chromatographic media and an inlet port and an outlet port coupled to the distribution network. A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.

A method for obtaining a liquid from a porous solid phase is described. The method comprises forming a liquid seal at a first end of a porous solid phase to which a liquid is bound, wherein liquid of the liquid seal is immiscible with the liquid bound to the solid phase, and applying a pressure differential across the porous solid phase to cause the immiscible liquid to move through the porous solid phase towards a second end of the porous solid phase, thereby displacing the liquid bound to the porous solid phase towards the second end and releasing this liquid from the second end. Recovery of liquid from the solid phase using such methods is increased compared with corresponding methods in which no liquid seal is formed. In preferred embodiments, the liquid used to form the liquid seal is a mineral oil. The methods have particular application in nucleic acid extractions which utilize capture of nucleic acid to a solid phase. Kits and apparatus for performing the methods are also described.