Apparatus and methods for performing chromatography may include a chromatography column and a vacuum insulated jacket having an inner wall and an outer wall. A vacuum area may be formed between the inner wall and the outer wall. The inner wall of the vacuum insulated jacket may surround the chromatography column. A gap may be formed between an outer wall of the chromatography column and the inner wall of the vacuum insulated jacket. The vacuum insulated jacket may extend beyond one or more end frits of the column. The gap may be filled with one or more materials so as to form an insulating or thermal barrier.

Apparatus and methods for performing chromatography may include a chromatography column and a vacuum insulated jacket having an inner wall and an outer wall. A vacuum area may be formed between the inner wall and the outer wall. The inner wall of the vacuum insulated jacket may surround the chromatography column. A gap may be formed between an outer wall of the chromatography column and the inner wall of the vacuum insulated jacket. The vacuum insulated jacket may extend beyond one or more end frits of the column. The gap may be filled with one or more materials so as to form an insulating or thermal barrier.

Herein is reported the use of a chromatography column support comprising at least one plane of symmetry, one axis of symmetry, at least three legs, at least three straight connectors, whereby the connectors define a plane that is perpendicular to the axis of symmetry of the support, whereby the connectors are connected to each other at the axis of symmetry, whereby each leg is connected to a connector, whereby each leg is perpendicular to the plane defined by the connectors, whereby all legs are on the same side of the plane defined by the connectors for stabilizing the packing of a chromatography column.

Herein is reported the use of a chromatography column support comprising at least one plane of symmetry, one axis of symmetry, at least three legs, at least three straight connectors, whereby the connectors define a plane that is perpendicular to the axis of symmetry of the support, whereby the connectors are connected to each other at the axis of symmetry, whereby each leg is connected to a connector, whereby each leg is perpendicular to the plane defined by the connectors, whereby all legs are on the same side of the plane defined by the connectors for stabilizing the packing of a chromatography column.

A combined device for sewage deep treatment integrally consists of an open box body (1) divided into multiple water treatment units (29) by a partition wall (27). Each water treatment unit (29) is divided into multiple grids by a partition wall (27′). The lower part of the box body (1) is provided with a conical structure (30) having a discharging door (22) thereunder. The partition wall (27) of the box body (1) has a water blocking belt (33) in the middle thereof, and the box body (1) has rails (31) mounted on two sides of the top thereof, rails (31) having an electric operating platform (2) mounted thereon. The box body (1) is filled with water processing granular carbon (32). Also disclosed is a sewage treatment method employing the combined sewage deep-treatment device. With the method, it is convenient to replace activated carbon, thereby solving the problem that it is difficult for the sewage treatment system to operate continuously and stably when replacing the activated carbon in traditional activated carbon filter.

A combined device for sewage deep treatment integrally consists of an open box body (1) divided into multiple water treatment units (29) by a partition wall (27). Each water treatment unit (29) is divided into multiple grids by a partition wall (27′). The lower part of the box body (1) is provided with a conical structure (30) having a discharging door (22) thereunder. The partition wall (27) of the box body (1) has a water blocking belt (33) in the middle thereof, and the box body (1) has rails (31) mounted on two sides of the top thereof, rails (31) having an electric operating platform (2) mounted thereon. The box body (1) is filled with water processing granular carbon (32). Also disclosed is a sewage treatment method employing the combined sewage deep-treatment device. With the method, it is convenient to replace activated carbon, thereby solving the problem that it is difficult for the sewage treatment system to operate continuously and stably when replacing the activated carbon in traditional activated carbon filter.

The present invention provides methods for analyzing a target compound from a biological sample. In one aspect, a method for analyzing a target compound in a biological sample can comprise delivering a biological sample through an affinity column, the affinity column having a binding ligand coupled to a stationary structural support, wherein the affinity column has a high density of the binding ligand per the stationary structural support and wherein the binding ligand has been preselected to cause weak affinity separation zonal retardation of the target compound from the biological sample forming a target compound fraction and a biological sample fraction and detecting the target compound by mass spectrometry.

The present invention provides methods for analyzing a target compound from a biological sample. In one aspect, a method for analyzing a target compound in a biological sample can comprise delivering a biological sample through an affinity column, the affinity column having a binding ligand coupled to a stationary structural support, wherein the affinity column has a high density of the binding ligand per the stationary structural support and wherein the binding ligand has been preselected to cause weak affinity separation zonal retardation of the target compound from the biological sample forming a target compound fraction and a biological sample fraction and detecting the target compound by mass spectrometry.

The present invention is directed to an affinity chromatography device that has a normal flow and which separates a targeted protein from aqueous mixtures. The chromatography device includes a housing containing therein a spiral wound membrane assembly that includes at least one inner intermediate material that forms an outer flow channel, at least one polymer membrane that contains therein inorganic particles, and at least one outer intermediate material that forms an inner flow channel sequentially positioned around a central core having a solid outer wall. An aqueous mixture is passed through the outer flow channel, through the polymer membrane where the targeted protein is removed, and then through an inner flow channel. The affinity chromatography device further includes an inlet flow distributor containing an inlet and an outlet flow distributer containing an outlet. Additionally, the chromatography device has a dimensionless resistance parameter that is less than 0.08.

The present invention is directed to an affinity chromatography device that has a normal flow and which separates a targeted protein from aqueous mixtures. The chromatography device includes a housing containing therein a spiral wound membrane assembly that includes at least one inner intermediate material that forms an outer flow channel, at least one polymer membrane that contains therein inorganic particles, and at least one outer intermediate material that forms an inner flow channel sequentially positioned around a central core having a solid outer wall. An aqueous mixture is passed through the outer flow channel, through the polymer membrane where the targeted protein is removed, and then through an inner flow channel. The affinity chromatography device further includes an inlet flow distributor containing an inlet and an outlet flow distributer containing an outlet. Additionally, the chromatography device has a dimensionless resistance parameter that is less than 0.08.