Chromatography method in which a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing, said packing being characterized in that: it comprises a plurality of capillary ducts extending in the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing—the material of the walls comprises a first population of connected pores, providing passages from one duct to the next enabling molecular diffusion to take place between adjacent ducts, pores having a mean diameter (d.sub.pore) of greater than 2 times the molecular diameter of at least one species to be separated—the diameter of the ducts is less than 50 μm.

Chromatography method in which a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing, said packing being characterized in that: it comprises a plurality of capillary ducts extending in the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing—the material of the walls comprises a first population of connected pores, providing passages from one duct to the next enabling molecular diffusion to take place between adjacent ducts, pores having a mean diameter (d.sub.pore) of greater than 2 times the molecular diameter of at least one species to be separated—the diameter of the ducts is less than 50 μm.

A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.

A biocompatible tube and fitting system that can be used in a liquid chromatography system is described. The tube can have a polymer tip and can be used in conjunction with one or more fitting assembly.

A chromatographic cassette includes a cassette including a chamber, chromatographic media disposed within the cassette chamber, a distribution network fluidly coupled to the chromatographic media and an inlet port and an outlet port coupled to the distribution network. A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.

A chromatographic cassette includes a cassette including a chamber, chromatographic media disposed within the cassette chamber, a distribution network fluidly coupled to the chromatographic media and an inlet port and an outlet port coupled to the distribution network. A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.

A chromatography and synthesis column that includes a main tube, a plurality of lower media ports, and an internal groove. The internal groove is formed in an interior surface of the main tube and selectively provides an internal flow path between each of the internal lower media ports. This, in turn, facilitates quick and easy maintenance of the internal lower media ports and other components of the chromatography and synthesis column.

A chromatography and synthesis column that includes a main tube, a plurality of lower media ports, and an internal groove. The internal groove is formed in an interior surface of the main tube and selectively provides an internal flow path between each of the internal lower media ports. This, in turn, facilitates quick and easy maintenance of the internal lower media ports and other components of the chromatography and synthesis column.

The present invention discloses methods for packing chromatography columns using dual-purpose valve assemblies. The key components of the dual-purpose valve comprise of the following; the inlet port (224) which sits on the first adaptor (212), the hollow nozzle (320) which passes through the first adaptor (212) cavity and is closed on the bottom end, and the internal movable plug (322). Different components of our de-vice work in way that the mobile phase and analyte(s) distribution is not blocked in the centre of the column, allowing for a uniform and homogeneous fluid distribution. This simple yet novel design offers uniform fluid distribution, stable packed bed, eliminates any void volume, and prevents bacterial contamination/growth. Ease of use, scalability, ease of cleaning, cost-efficiency and fine-tuning packing conditions such as pressure are key features of this invention.

The present invention discloses methods for packing chromatography columns using dual-purpose valve assemblies. The key components of the dual-purpose valve comprise of the following; the inlet port (224) which sits on the first adaptor (212), the hollow nozzle (320) which passes through the first adaptor (212) cavity and is closed on the bottom end, and the internal movable plug (322). Different components of our de-vice work in way that the mobile phase and analyte(s) distribution is not blocked in the centre of the column, allowing for a uniform and homogeneous fluid distribution. This simple yet novel design offers uniform fluid distribution, stable packed bed, eliminates any void volume, and prevents bacterial contamination/growth. Ease of use, scalability, ease of cleaning, cost-efficiency and fine-tuning packing conditions such as pressure are key features of this invention.