A preparative liquid chromatograph includes a liquid chromatograph section, a trap section, an eluent supply section, a collector, and a flow path switching section. The flow path switching section is configured to be selectively switched to a component trap mode that connects the liquid chromatograph section and the trap section in such a way that a sample component separated in a separation column is trapped by a trap column of the trap section; and a collection mode that connects the eluent supply section and the trap section and connects the trap section and the collector in such a way that the components trapped in the trap column are eluted by an eluent from the eluent supply section and are guided to the collector.

The present invention is related a method of purifying a heterologous protein from an egg white. In some embodiments, the disclosure is directed to a method of purifying a heterologous protein from an egg white comprising the heterologous protein, the method comprising, (a) adjusting the pH of the egg white to a pH of 5.8 to 6.5 to form a pH-adjusted egg white; (b) filtering the pH-adjusted egg white of (a) and collecting a first filtrate; (c) subjecting the first filtrate of (b) to a hydrophobic interaction chromatography matrix, and collecting a first eluate comprising the heterologous protein; (d) adjusting the pH of the first eluate of (c) to a pH of 5.0 to 5.6 to form a pH-adjusted eluate; (e) filtering the pH-adjusted eluate to obtain a second filtrate; (f) adjusting the pH of the second filtrate to a pH of 3.0 to 4.0 to form a pH-adjusted second filtrate; (g) neutralizing the pH-adjusted second filtrate of (f) to a pH of 5.0 to 8.0 to form a neutralized solution; (h) subjecting the neutralized solution to a cation exchange chromatography matrix and collecting a second eluate comprising the heterologous protein.

The present invention is related a method of purifying a heterologous protein from an egg white. In some embodiments, the disclosure is directed to a method of purifying a heterologous protein from an egg white comprising the heterologous protein, the method comprising, (a) adjusting the pH of the egg white to a pH of 5.8 to 6.5 to form a pH-adjusted egg white; (b) filtering the pH-adjusted egg white of (a) and collecting a first filtrate; (c) subjecting the first filtrate of (b) to a hydrophobic interaction chromatography matrix, and collecting a first eluate comprising the heterologous protein; (d) adjusting the pH of the first eluate of (c) to a pH of 5.0 to 5.6 to form a pH-adjusted eluate; (e) filtering the pH-adjusted eluate to obtain a second filtrate; (f) adjusting the pH of the second filtrate to a pH of 3.0 to 4.0 to form a pH-adjusted second filtrate; (g) neutralizing the pH-adjusted second filtrate of (f) to a pH of 5.0 to 8.0 to form a neutralized solution; (h) subjecting the neutralized solution to a cation exchange chromatography matrix and collecting a second eluate comprising the heterologous protein.

A method for preparing a Luo Han Guo sweetening composition from Siraitia grosvenorii and a use thereof. The method for extracting the sweetening composition from Siraitia grosvenorii preferably includes the followings: accelerating ripening of immature Siraitia grosvenorii, and performing juicing, extraction with pure water, removal of impurities, concentration and purification to obtain the sweetening composition. Further, the present application relates to a compound sweetener containing the sweetening composition, which can be widely used in foodstuffs, beverages, healthcare products, and daily chemicals. The contents of mogroside III, mogroside IIe, and the like in the Luo Han Guo sweetening composition are controlled so as to improve the flavor thereof, and a production process for the sweetening composition uses only pure water, without use of organic solvents such as ethanol, to ensure a greener and healthier production process.

Systems and methods for efficient, effective, and safe separation and isolation of multiple isotopes (e.g., Mo, Zr, Ba, Sr, Te, and lanthanide isotopes) from fission products includes use of a plurality of chromatography columns, each containing a chromatographic resin formulated to target one or more particular isotopes. The system is operable in a “series” configuration to load the multiple columns by a single pass of the sample. Then, the system may be transitioned (e.g., using valves) to a “parallel” configuration in which multiple columns of the system may be operated simultaneously to elute targeted isotopes. Additional parallel operations of the columns, using different eluent compositions, may be used to elute different targeted isotopes. The system may be reconditioned in preparation for a subsequent sample.

Systems and methods for efficient, effective, and safe separation and isolation of multiple isotopes (e.g., Mo, Zr, Ba, Sr, Te, and lanthanide isotopes) from fission products includes use of a plurality of chromatography columns, each containing a chromatographic resin formulated to target one or more particular isotopes. The system is operable in a “series” configuration to load the multiple columns by a single pass of the sample. Then, the system may be transitioned (e.g., using valves) to a “parallel” configuration in which multiple columns of the system may be operated simultaneously to elute targeted isotopes. Additional parallel operations of the columns, using different eluent compositions, may be used to elute different targeted isotopes. The system may be reconditioned in preparation for a subsequent sample.

Systems and methods for efficient, effective, and safe separation and isolation of multiple isotopes (e.g., Mo, Zr, Ba, Sr, Te, and lanthanide isotopes) from fission products includes use of a plurality of chromatography columns, each containing a chromatographic resin formulated to target one or more particular isotopes. The system is operable in a “series” configuration to load the multiple columns by a single pass of the sample. Then, the system may be transitioned (e.g., using valves) to a “parallel” configuration in which multiple columns of the system may be operated simultaneously to elute targeted isotopes. Additional parallel operations of the columns, using different eluent compositions, may be used to elute different targeted isotopes. The system may be reconditioned in preparation for a subsequent sample.

Systems and methods for efficient, effective, and safe separation and isolation of multiple isotopes (e.g., Mo, Zr, Ba, Sr, Te, and lanthanide isotopes) from fission products includes use of a plurality of chromatography columns, each containing a chromatographic resin formulated to target one or more particular isotopes. The system is operable in a “series” configuration to load the multiple columns by a single pass of the sample. Then, the system may be transitioned (e.g., using valves) to a “parallel” configuration in which multiple columns of the system may be operated simultaneously to elute targeted isotopes. Additional parallel operations of the columns, using different eluent compositions, may be used to elute different targeted isotopes. The system may be reconditioned in preparation for a subsequent sample.

A method of recovering paraxyiene in a pressure swing adsorption unit with varying hydrogen purge pressures. The pressure swing adsorption zone is adapted to adsorb and desorb paraxyiene based on the cycling of partial pressure in the zone. A first hydrogen purge fed to the zone is within 50 psi of the adsorption pressure of paraxyiene in the zone. A second hydrogen purge fed to the zone is within 50 psi of the desorption pressure of paraxyiene in the zone. The overall amount of hydrogen necessary to operate the pressure swing adsorption zone is reduced and heat may be recovered from the effluent leaving the pressure swing adsorption zone.

A process for producing a TCH stream, the process comprising: separating, in a first column, an adiponitrile process stream comprising TCH and optionally adiponitrile, to form an adiponitrile stream comprising greater than 5 wt. % adiponitrile and a first TCH stream comprising TCH, and optionally a heavies stream comprising high-boiling components and solid impurities; and optionally purifying the first TCH stream, via one or more columns, to form a purified TCH stream comprising greater than 50 wt. % TCH; wherein the first column is operated at a pressure drop less than 25 mmHg.