The invention concerns biocompatible polymer systems comprising at least one polymer with a plurality of pores, said polymer comprising either polyol or zwitterionic groups designed to adsorb endotoxins and other inflammatory mediator molecules. The inventions are in the field of porous polymeric sorbents, also in the field of broadly reducing endotoxins in blood and blood products that can cause endotoxemia, additionally, in the field of broadly removing endotoxins by perfusion or hemoperfusion.

The invention concerns biocompatible polymer systems comprising at least one polymer with a plurality of pores, said polymer comprising either polyol or zwitterionic groups designed to adsorb endotoxins and other inflammatory mediator molecules. The inventions are in the field of porous polymeric sorbents, also in the field of broadly reducing endotoxins in blood and blood products that can cause endotoxemia, additionally, in the field of broadly removing endotoxins by perfusion or hemoperfusion.

This disclosure relates to the use of size exclusion chromatography and/or size exclusion chromatography with multi-angle light scattering technology to characterize viral particles such as adeno-associated virus and lentivirus particles. The disclosed methods are also useful for estimating the titer of viral particles, determining the integrity of the viral particles and estimating the amount of DNA encapsidated in the viral particle.

This disclosure relates to the use of size exclusion chromatography and/or size exclusion chromatography with multi-angle light scattering technology to characterize viral particles such as adeno-associated virus and lentivirus particles. The disclosed methods are also useful for estimating the titer of viral particles, determining the integrity of the viral particles and estimating the amount of DNA encapsidated in the viral particle.

A method and system are disclosed for isolating intact acellular particles using size exclusion and for obtaining size and concentration of such isolated particles. In one embodiment, the disclosure is directed to use of Particle Purification Liquid Chromatography (PPLC), a high-resolution chromatographic size-guided turbidimetry-enabled system for dye-free isolation, on-line characterization, and retrieval of intact acellular particles, including extracellular vesicles (EVs) and membraneless condensate particles (MCs) from various biofluids.

A method and system are disclosed for isolating intact acellular particles using size exclusion and for obtaining size and concentration of such isolated particles. In one embodiment, the disclosure is directed to use of Particle Purification Liquid Chromatography (PPLC), a high-resolution chromatographic size-guided turbidimetry-enabled system for dye-free isolation, on-line characterization, and retrieval of intact acellular particles, including extracellular vesicles (EVs) and membraneless condensate particles (MCs) from various biofluids.

A separation medium for removing small molecules from biomacromolecule in aqueous mixtures comprises gel filtration chromatography beads having a nominal protein fractional range of about 1000 Da to about 5000 Da and having an internal adsorbent matrix derived from a hydrophobicized scaffold. The gel filtration chromatography beads remove small molecules that are less than 1500 Da and have log Pow values greater than about −0.5 from biomacromolecules in aqueous mixtures. Devices containing the separation medium are also provided.

A separation medium for removing small molecules from biomacromolecule in aqueous mixtures comprises gel filtration chromatography beads having a nominal protein fractional range of about 1000 Da to about 5000 Da and having an internal adsorbent matrix derived from a hydrophobicized scaffold. The gel filtration chromatography beads remove small molecules that are less than 1500 Da and have log Pow values greater than about −0.5 from biomacromolecules in aqueous mixtures. Devices containing the separation medium are also provided.

Materials and methods for use of constrained cohydration agents in the purification of biological materials such as antibodies, viruses, cells, and cellular organelles in connection with convective chromatography, fluidized bed or co-precipitation applications.

Materials and methods for use of constrained cohydration agents in the purification of biological materials such as antibodies, viruses, cells, and cellular organelles in connection with convective chromatography, fluidized bed or co-precipitation applications.