In one aspect, separation media are described herein operable for removing one or more water contaminants including NOM and derivatives thereof. Briefly, a separation medium includes a nanoparticle support and an oligomeric stationary phase forming a film on individual nanoparticles of the support, the film having thickness of 1 to 100 nm. In some embodiments, oligomeric chains of the stationary phase are covalently bonded to the individual nanoparticles.

An ion exchange device main body 3 includes: a tubular body 31 into which an ion exchange resin bag 5 accommodating the ion exchange resin is inserted through an opening and which has a liquid outlet 312 in which a liquid outlet port 314 for discharging an ion exchange target liquid to outside is formed; a lid 32 that is supported by the tubular body 31 and has a gas injection portion 324 in which a gas injection port 325 for injecting a to an inside 311 of the tubular body 31 is formed; a lead-out pipe 42 that is connected to the liquid outlet 312 and guides the ion exchange target liquid to the outside; and a check valve 44 that is provided in the lead-out pipe 42 and prevents the ion exchange target liquid from flowing backward from the outside to the inside.

The invention relates to a new process for the purification of oligonucleotides which comprises the removal of an acid labile 5′hydroxy protecting group at the 5′-O-oligonucleotide terminus of the oligonucleotide by means of an on-column de-protection with an acid.

The present invention relates to a method of preparing kestose-containing fructooligosaccharide, and more specifically, a method of preparing kestose-containing fructooligosaccharide having a high content of kestose and excellent storage stability.

A purification device is provided that includes a porous container, purification media retained in the porous container, and a flow controller integral to the porous container. A purification device is also provided that includes a porous elastic container, purification media, and a flow controller. The porous elastic container has a pocket formed therein. The purification media is compressibly retained in the porous elastic container. The flow controller is elastically retained in the pocket of the porous elastic container.

An ion suppressor includes ion exchange membranes between a pair of electrodes. Regeneration liquid channels are provided in the spaces between the electrodes and the ion exchange membranes, and an eluent channel is provided between the ion exchange membranes. Ion re-exchange in the eluent on the downstream side of the eluent channel is suppressed, thereby making it possible to improve the detection sensitivity for the ion to be measured. For example, the eluent channel has a folded structure, thereby increasing the amount of current on the downstream side of the eluent channel, and thus, the accumulation of ions is suppressed, and accordingly, ion re-exchange in the eluent can be suppressed.

Method and system for purifying a sample comprising a biomolecule of interest and impurities, comprising expressing said biomolecule of interest in a bioreactor to form a product sample comprising said biomolecule of interest and impurities; subjecting said product sample to filtration to form a clarified product sample; subjecting said clarified product sample to affinity chromatography to remove impurities; subsequently subjecting said product sample to diafiltration followed by virus filtration and optional concentration. The buffer used during the diafiltration step (and thus in the virus filtration step) is the buffer desired for the final formulation of the product.

Methods of producing multiple protein products from blood-based materials including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins are described herein. The inventive methods include steps of fractionation that utilize a combination of salt and organic solvent. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields. The sequence of process steps can be selected to obtain multiple products from various in-process materials, such as supernatants, pastes, chromatography flow-though, and chromatography washes.

Functionalized particulate support material and chromatographic media prepared therefrom are disclosed. The functionalized particulate support material is a plurality of particles, each particle having a particle surface. Chemically bonded to and extending from the surface of the particles is a combination of hydrophobic and hydrophilic functional groups. The hydrophobic functional groups enable polymerization of one or more monomers onto the particle surface while the hydrophilic functional groups provide increased wettability of the particle surface compared to an unmodified particle surface. The functionalized particulate support material may be further processed so as to form polymer chains extending from the hydrophobic functional groups. In one embodiment, the resulting polymer functionalized material is useful as a chromatographic media in chromatography columns or cartridges, such as in a liquid chromatography (HPLC) column. Chromatography columns or cartridges containing the polymer functionalized media, and methods of making and using the media, are also disclosed.

A device for forming a liquid treatment apparatus includes a main part, a receiving part including a cavity for receiving a connecting head of a replaceable liquid treatment cartridge having at least one port in liquid communication with an interior of the liquid treatment cartridge. The receiving part is journalled for movement between a first and a second position with respect to the main part. The head is insertable into and retractable from the cavity in the first position. The liquid treatment cartridge is lockable to the device by at least moving the receiving part with the inserted connecting head into the second position. The movement includes a component corresponding to an intrinsic rotation in a plane parallel to a direction of insertion. The movement further includes at least a component corresponding to a displacement of the receiving part relative to the main part.