Chromatography method in which a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing, said packing being characterized in that: it comprises a plurality of capillary ducts extending in the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing—the material of the walls comprises a first population of connected pores, providing passages from one duct to the next enabling molecular diffusion to take place between adjacent ducts, pores having a mean diameter (d.sub.pore) of greater than 2 times the molecular diameter of at least one species to be separated—the diameter of the ducts is less than 50 μm.

Chromatography method in which a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing, said packing being characterized in that: it comprises a plurality of capillary ducts extending in the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing—the material of the walls comprises a first population of connected pores, providing passages from one duct to the next enabling molecular diffusion to take place between adjacent ducts, pores having a mean diameter (d.sub.pore) of greater than 2 times the molecular diameter of at least one species to be separated—the diameter of the ducts is less than 50 μm.

The invention discloses a detection method based on supercritical fluid chromatography (SFC) and post-column dicationic ionic liquid (DIL) charge complexation, which includes the following steps: (1) The supramolecular solvent (SUPRAS) was prepared by mixing heptanol, tetrahydrofuran, and water; (2) Sample pretreatment: the SUPRAS was used to extract the sample for subsequent analysis; (3) Analysis of perfluorinated compounds (PFCs) using SFC separation, post-column DIL-based charge complexation, and electrospray ionization-mass spectrometry (ESI-MS). The invention established a novel analytical method for the detection of PFCs in textiles incorporating post-chromatographic DIL-based charge complexation and SFC coupled with ESI-MS. The DIL reagent formed positively charged complexes with anionic analytes during the ESI process, facilitating MS detection in the positive ion mode with enhanced detection sensitivity.

The invention discloses a detection method based on supercritical fluid chromatography (SFC) and post-column dicationic ionic liquid (DIL) charge complexation, which includes the following steps: (1) The supramolecular solvent (SUPRAS) was prepared by mixing heptanol, tetrahydrofuran, and water; (2) Sample pretreatment: the SUPRAS was used to extract the sample for subsequent analysis; (3) Analysis of perfluorinated compounds (PFCs) using SFC separation, post-column DIL-based charge complexation, and electrospray ionization-mass spectrometry (ESI-MS). The invention established a novel analytical method for the detection of PFCs in textiles incorporating post-chromatographic DIL-based charge complexation and SFC coupled with ESI-MS. The DIL reagent formed positively charged complexes with anionic analytes during the ESI process, facilitating MS detection in the positive ion mode with enhanced detection sensitivity.

A supercritical fluid apparatus includes a solvent supplier that supplies a solvent, a pressure control device provided in a flow path for a solvent supplied by the solvent supplier, and a controller that controls the pressure control device. The controller includes a first controller that increases a pressure in the flow path, puts the solvent in a supercritical fluid state and maintains an environment for execution of a predetermined process by controlling the pressure control device, and a second controller that sets an intermediate target value for a pressure in the flow path and controls a pressure in the flow path in order to get the pressure to reach the intermediate target value, when ending the environment for execution of a predetermined process.

A liquid carbon dioxide supply device is configured to supply liquid carbon dioxide to a supercritical fluid apparatus including a separation column, and includes a first flow path, a second flow path, a compressor, a heat exchanger and a pump. The compressor circulates a first refrigerant through the first flow path such that a refrigerant cycle is repeated. The heat exchanger exchanges heat between the first flow path and the second flow path. The pump supplies liquid carbon dioxide flowing through the second flow path to the separation column of the supercritical fluid apparatus.

The invention relates to a method of chromatography wherein a gaseous, liquid or supercritical fluid mobile phase, which contains substances to be separated, flows through a porous packing which comprises a plurality of capillary channels which extend in the direction of flow of said mobile phase, said packing being manufactured by a method wherein: a bundle of elementary fibres is assembled, said fibres comprising a core made of a solid, liquid or gaseous material, and a shell made of a drawable material, said bundle is drawn in order to reduce the diameter of said fibres, a porous matrix is formed around the core of the drawn fibres, the formation of said porous matrix comprising a transformation of the shell material, where said porous matrix comprises at least one population of connected pores interconnecting the channels, where the thickness of the porous matrix between two adjacent channels is less than the diameter of the channels, preferably less than half the diameter of the channels, where necessary the core material is removed so as to leave free channels in the porous matrix.

The invention relates to a method of chromatography wherein a gaseous, liquid or supercritical fluid mobile phase, which contains substances to be separated, flows through a porous packing which comprises a plurality of capillary channels which extend in the direction of flow of said mobile phase, said packing being manufactured by a method wherein: a bundle of elementary fibres is assembled, said fibres comprising a core made of a solid, liquid or gaseous material, and a shell made of a drawable material, said bundle is drawn in order to reduce the diameter of said fibres, a porous matrix is formed around the core of the drawn fibres, the formation of said porous matrix comprising a transformation of the shell material, where said porous matrix comprises at least one population of connected pores interconnecting the channels, where the thickness of the porous matrix between two adjacent channels is less than the diameter of the channels, preferably less than half the diameter of the channels, where necessary the core material is removed so as to leave free channels in the porous matrix.

The flow cell of the present application simultaneously monitors and measures light absorbance and fluorescence of particles in a flowing liquid. The flow cell comprises a housing having a light input face, an absorbance output face and first and second emission output faces; a fluid flow section within the housing that comprises a bottom funnel through which fluid enters the flow cell, a core chamber into which fluid flows from the bottom funnel, and a top funnel into which fluid flows from the core chamber, wherein the bottom and top funnels each comprise a first end which extends at an angle to a second end that is wider in diameter than the first end, and said second end of each is adjacent to and aligned with the core chamber; and a center section within the housing center having a recess formed therein which houses the core chamber of the fluid flow section, wherein said center section comprises a first pair of opposing channels formed in the light input face and the absorbance output face, respectively, and a second pair of opposing channels formed in the first emission output face and the second emission output face and which are perpendicular to the first pair of opposing channels, and wherein the first pair of opposing channels and second pair of opposing channels are in communication with the core chamber. An apparatus comprising the flow cell is also provided.

The flow cell of the present application simultaneously monitors and measures light absorbance and fluorescence of particles in a flowing liquid. The flow cell comprises a housing having a light input face, an absorbance output face and first and second emission output faces; a fluid flow section within the housing that comprises a bottom funnel through which fluid enters the flow cell, a core chamber into which fluid flows from the bottom funnel, and a top funnel into which fluid flows from the core chamber, wherein the bottom and top funnels each comprise a first end which extends at an angle to a second end that is wider in diameter than the first end, and said second end of each is adjacent to and aligned with the core chamber; and a center section within the housing center having a recess formed therein which houses the core chamber of the fluid flow section, wherein said center section comprises a first pair of opposing channels formed in the light input face and the absorbance output face, respectively, and a second pair of opposing channels formed in the first emission output face and the second emission output face and which are perpendicular to the first pair of opposing channels, and wherein the first pair of opposing channels and second pair of opposing channels are in communication with the core chamber. An apparatus comprising the flow cell is also provided.