The present invention provides novel methods for reducing the serine protease and/or serine protease zymogen content of a plasma-derived protein composition. Also provided are methods for manufacturing plasma-derived protein compositions having reduced serine protease and\or serine protease zymogen content. Among yet other aspects, the present invention provides aqueous and lyophilized compositions of plasma-derived proteins having reduced serine protease and/or serine protease zymogen content. Yet other aspects include methods for treating, managing, and/or preventing a disease comprising the administration of a plasma-derived protein composition having a reduced serine protease or serine protease zymogen content.

The invention provides a method for the purification of complexed .sup.227Th from a mixture comprising complexed .sup.227Th and .sup.223Ra (complexed or in solution), said method comprising: i) preparing a first solution comprising a mixture of complexed .sup.227Th ions and .sup.223Ra ions in a first aqueous buffer; ii) loading said first solution onto a separation material; iii) eluting complexed .sup.227Th from said separation material whereby to generate a second solution comprising complexed .sup.227Th; iv) Optionally rinsing said separation material using a first aqueous washing medium;

The invention additionally provides a purified .sup.227Th solution, a pharmaceutical product and its use in treatment of disease such as cancer and a kit for generation of such a product.

A vitamin E production method and a vitamin E production device which can highly purify vitamin E in a vitamin E concentrated fraction are provided. A raw oil supply section supplies a raw oil to a series column in which two or more columns including a strongly basic anion exchanger are coupled in series to adsorb vitamin E included in the raw oil on the strongly basic anion exchanger of at least one column from among the series column. A desorption solution supply section supplies a desorption solution to a column on which vitamin E has been adsorbed to desorb vitamin E from the strongly basic anion exchanger of the column.

The invention relates to a method for purifying a fructose-containing mixture to be separated in a multicolumn chromatography system, the method comprising successively and cyclically: a step of collecting a raffinate, a step of injecting the mixture to be separated, a step of collecting an extract and a step of injecting eluent;
wherein the mixture to be separated has a dry matter mass concentration of 45 to 55%, the method being carried out at a temperature of 50 to 62° C.

The present invention describes a simple purification process for recombinant human serum albumin. The process results in highly purified protein with limited number of purification steps. The broth containing human albumin is clarified by centrifugation and microfiltration, diafiltered and captured by cation exchange chromatography by a process that allows 140-230 mg of albumin to be captured per ml of resin. Product related impurities are removed by hydrophobic interaction chromatography, optimised to allow 87-97% recovery in flow through mode. The final series of processes are so combined that there is easy transition from one step to the next with minimal interventions and adjustments. The entire process of purification is completed within two days from harvest to final product. Thus a cost-effective process with improved recovery of protein at each step is developed. The purified human serum albumin is analyzed for purity and shows physicochemical characteristics that are similar to standard albumin.

The invention relates to the field of protein purification processes involving several chromatography steps. The invention pertains to a method for purifying a protein, preferably an antibody or fragment thereof or a protein containing said fragment, from a complex solution, wherein said method comprises at least two chromatography steps which are performed using buffers comprising or consisting of the same chemical compounds. The invention is particularly useful for large scale production and purification of recombinant proteins.

The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.

A chromatographic separation method for separating plural components contained in a liquid to be separated by a chromatography, with a separating device 1 including plural filling portions 10 filled with a separating agent for separating the plural components contained in the liquid to be separated, a supply portion 20 provided in each of the plural filling portions 10 to supply the liquid to be separated or an eluent for extracting any component contained in the liquid to be separated to the filling portion 10, and an extraction portion 30 to extract any component contained in the liquid to be separated from the filling portion 10, the method including: an upward supplying and extracting step of extracting any component contained in the liquid to be separated from an upward stream extraction portion while supplying the eluent to at least one filling portion 10 from an upward stream supply portion by an upward stream.

A spiral tube countercurrent chromatography rotor for separating virus in a two part aqueous solvent is described.

The present disclosure provides methods for the separation and quantification of empty and full viral capsids (e.g., AAV capsids) within a viral preparation, such as a viral pharmaceutical composition or drug product.