There are provide a cell separation filter with which cells can be separated without damage and can suppress adsorption, a filtering device, and a manufacturing method for a cell separation filter. The cell separation filter is composed of a nonwoven fabric that is formed of fibers containing a water-insoluble polymer and a hydrophilizing agent and has a fiber density difference in the film thickness direction. The nonwoven fabric has an average through-hole diameter of 2.0 μm or more and less than 10.0 μm, a void ratio of 75% or more and 98% or less, a film thickness of 100 μm or more, and a critical wet surface tension of 72 mN/m or more.

Provided are a hydrophilic porous membrane including a porous membrane and a hydroxyalkyl cellulose retained in the porous membrane, in which the average pore size differs between two surfaces of the porous membrane, the hydroxyalkyl cellulose distributed in the thickness direction of the hydrophilic porous membrane exhibits two or more peaks of detection intensity in GPC, and the weight-average molecular weight Mw.sub.min of the peak that is detected latest among the above-mentioned peaks is less than 100,000; and a method for producing a hydrophilic porous membrane, the method including separately preparing a hydrophilizing liquid including a hydroxyalkyl cellulose having a smaller weight-average molecular weight and a hydrophilizing liquid including a hydroxyalkyl cellulose having a larger weight-average molecular weight, and applying each of the hydrophilizing liquids on two surfaces of the porous membrane or sequentially on one surface thereof.

The present invention relates to method for producing and purifying RNA comprising the steps of providing DNA encoding the RNA; transcription of the DNA into RNA; and conditioning and/or purifying of the solution comprising transcribed RNA by one or more steps of tangential flow filtration (TFF).

The present invention relates to method for producing and purifying RNA comprising the steps of providing DNA encoding the RNA; transcription of the DNA into RNA; and conditioning and/or purifying of the solution comprising transcribed RNA by one or more steps of tangential flow filtration (TFF).

The present invention relates to method for producing and purifying RNA comprising the steps of providing DNA encoding the RNA; transcription of the DNA into RNA; and conditioning and/or purifying of the solution comprising transcribed RNA by one or more steps of tangential flow filtration (TFF).

The present invention relates to method for producing and purifying RNA comprising the steps of providing DNA encoding the RNA; transcription of the DNA into RNA; and conditioning and/or purifying of the solution comprising transcribed RNA by one or more steps of tangential flow filtration (TFF).

The present invention relates to method for producing and purifying RNA comprising the steps of providing DNA encoding the RNA; transcription of the DNA into RNA; and conditioning and/or purifying of the solution comprising transcribed RNA by one or more steps of tangential flow filtration (TFF).

The present invention relates to method for producing and purifying RNA comprising the steps of providing DNA encoding the RNA; transcription of the DNA into RNA; and conditioning and/or purifying of the solution comprising transcribed RNA by one or more steps of tangential flow filtration (TFF).

The present invention relates to method for producing and purifying RNA comprising the steps of providing DNA encoding the RNA; transcription of the DNA into RNA; and conditioning and/or purifying of the solution comprising transcribed RNA by one or more steps of tangential flow filtration (TFF).

The present invention relates to method for producing and purifying RNA comprising the steps of providing DNA encoding the RNA; transcription of the DNA into RNA; and conditioning and/or purifying of the solution comprising transcribed RNA by one or more steps of tangential flow filtration (TFF).