An apparatus, buffer solutions and a method are provided for pH control of in vitro dissolution tests used to monitor the drug release rate from solid unit dosage forms which are used to predict their in vivo effects or for quality control purposes. A method of preparing a continuous condition and a clear bicarbonate ion based solution for in vitro dissolution testing of pharmaceutical products is also provided. An enclosure device is also provided for use in the provision of pH control and stabilization to a bicarbonate based solution used in the in vitro dissolution testing of pharmaceutical products.

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

A bubble counter includes a body. The body is provided with a gas inlet, a gas outlet, a cavity, a water inlet, a water inlet channel, a first check valve and a second check valve. An end of the water inlet channel is connected to the water inlet, and another end of the water inlet channel is connected to the cavity. The first check valve is arranged between the gas inlet and the cavity, and the second check valve is arranged at a connection between the water inlet channel and the cavity. The water inlet channel and the gas outlet are independent from each other. With the bubble counter, it is not required to turn off or disassemble the bubble counter, water can be added at any time and gas leakage can be prevented.

The mixing nozzle has a throat section, a diffuser section, a gas nozzle section, a first liquid suction port, a liquid nozzle section, a second liquid suction port, a baffle plate, and a jetting port. The first liquid suction port liquidly absorbs the solution in the water storage pool from a side of the gas nozzle section toward the gas nozzle tip. The liquid nozzle section extends to the downstream side of the gas nozzle section with intervening the first liquid suction port. The second liquid suction port liquidly absorbs the solution in the water storage pool from a side of the liquid nozzle section toward the liquid nozzle tip. The baffle plate is provided such that the mixed flow mixed in the diffuser section collides in front of a downstream end of the diffuser section, and divides and reverses the mixed flow.

Embodiments described generally relate to systems comprising a humidifier (e.g., a bubble column humidifier) and a heating device (e.g. a heat exchanger), and associated methods. In certain embodiments, the heating device heats a first liquid stream comprising a condensable fluid in liquid phase (e.g., water) and a dissolved salt (e.g., NaCl) to a relatively low temperature (e.g., about 90° C. or less) prior to the first liquid stream entering the humidifier through a main humidifier liquid inlet. In some cases, the system comprising the humidifier and the heating device requires only low-grade heat to operate, which may be advantageous due to the low cost and high availability of such heat.

Various embodiments relate to an aeration device for a bioprocessing installation, in particular a bioreactor, comprising a housing, wherein the housing comprises one or more aeration channels in its interior, wherein the housing further comprises one or more gas inlet ports, via each of which a gas can be introduced into at least one aeration channel of the housing, and wherein the housing further comprises a plurality of gas outlet ports, via each of which the gas can be discharged from the respective aeration channel to the outside of the aeration device. It is proposed that the aeration device is configurable with respect to a predetermined discharge of gas via the gas outlet ports.

A blow-molding plastic bubble-generating device is provided, including a board-like shell body. The board-like shell body is formed through blowing a hollow embryo placed in a mold, the hollow embryo is blown and expanded, the hollow embryo is penetrated through by a plurality of needle posts on an inner wall of the mold to form the board-like shell body, the board-like shell body is integrally formed and hollow and has a shell wall which completely and continuously surrounds the board-like shell body, and the shell wall has a plurality of through holes.

A chemical liquid preparation method of preparing a chemical liquid for treating a film formed on a substrate, including a gas dissolving process in which an oxygen-containing gas and an inert-gas-containing gas are dissolved in the chemical liquid by supplying the oxygen-containing gas which contains oxygen gas and the inert-gas-containing gas which contains an inert gas to a chemical liquid, wherein in the gas dissolving process, a dissolved oxygen concentration in the chemical liquid is adjusted by setting a mixing ratio between the oxygen-containing gas and the inert-gas-containing gas supplied to the chemical liquid as a mixing ratio corresponding to a predetermined target dissolved oxygen concentration.

A chemical liquid preparation method of preparing a chemical liquid for treating a film formed on a substrate, including a gas dissolving process in which an oxygen-containing gas and an inert-gas-containing gas are dissolved in the chemical liquid by supplying the oxygen-containing gas which contains oxygen gas and the inert-gas-containing gas which contains an inert gas to a chemical liquid, wherein in the gas dissolving process, a dissolved oxygen concentration in the chemical liquid is adjusted by setting a mixing ratio between the oxygen-containing gas and the inert-gas-containing gas supplied to the chemical liquid as a mixing ratio corresponding to a predetermined target dissolved oxygen concentration.

A system and method of injecting a gas enriched and/or emulsified first liquid into a second liquid is disclosed. The injection can cause generation of a high density of bubbles having a mean diameter of a selected size. The mean diameter of the bubbles can be selected and varied based on the characteristics of the injection system.