The present invention concerns a stirrer system for animal cell culture consisting of a combination of at least one radially-conveying stirrer element and at least one axially-conveying stirrer element, wherein at least three stirrer elements must be present and the uppermost stirrer element is an axially-conveying stirrer element. The stirrer elements are arranged at a certain distance above one another on a stirrer shaft. A particular embodiment is a multiple stirrer system consisting of two disk stirrers as radially-conveying stirrer elements and an inclined-blade stirrer as an axially-conveying stirrer element wherein the inclined-blade stirrer is arranged above the disk stirrer on the stirrer shaft. The stirrer system according to the invention achieves among others a gentler and better intermixing in the culture of shear-sensitive mammalian cells in cell cultures.

A stirring unit for stirring a fluid contained in a vessel includes a mixing body for stirring the fluid by rotating around a rotation axis, and a magnet or a magnetic substance for receiving a rotating magnetic field for rotating the mixing body, in which a suction port and a discharge port for the fluid are provided on a surface of the mixing body, a plurality of flow paths connecting the suction port and the discharge port are provided inside the mixture, the suction port is disposed at a position on the rotation axis or at a position closer to the rotation axis than the discharge port, and the discharge port is disposed at a position outside the rotation axis than the suction port.

A disposable container, such as a deformable bag, for a fluid, having one or more inlets and one or more outlets and an impeller assembly within the container to cause mixing, dispersing, homogenizing and/or circulation of one or more ingredients contained or added to the container. The region within the container that can contain liquid is funnel shaped, which allows very low fluid level mixing, dispensing while mixing, and a reduction or elimination of vortex formation.

A disposable container, such as a deformable bag, for a fluid, having one or more inlets and one or more outlets and an impeller assembly within the container to cause mixing, dispersing, homogenizing and/or circulation of one or more ingredients contained or added to the container. The region within the container that can contain liquid is funnel shaped, which allows very low fluid level mixing, dispensing while mixing, and a reduction or elimination of vortex formation.

A mixing unit has a stacked member having mixing elements that are stacked in a stacking direction and that extend in an extending direction, a first plate, and a second plate disposed opposite the first plate. The stacked member is sandwiched between the first plate and the second plate. Each of the mixing elements has first through holes. The second plate comprises an opening portion that communicates with the first through holes in the stacked member.

A fluid path set includes a first fluid line having a proximal end fluidly connectable to a source of a first fluid and a second fluid line having a proximal end fluidly connectable to a source of a second fluid. A flow mixing device is in fluid communication with distal ends of the first and second fluid lines. The flow mixing device includes a housing, a first fluid port provided for receiving the first fluid, and a second fluid port for receiving the second fluid. A mixing chamber is disposed within the housing and is in fluid communication with the first and second fluid ports. A third fluid port in fluid communication with the mixing chamber for discharging a mixed solution of the first and second fluids. A turbulent flow inducing member is disposed within the mixing chamber for promoting turbulent mixing of the first and second fluids.

A device and apparatus for mixing and dispersing a solid and a liquid are provided. The device includes a powder pulverizing mechanism fixedly connected to a main shaft. The powder pulverizing mechanism is located in a powder feed chamber. The powder feed chamber is provided with a powder feed port. A liquid feed chamber is arranged on an outer side of the powder feed chamber. The liquid feed chamber is provided with a liquid feed port, and the liquid feed chamber is provided therein with a dispersing mechanism fixedly connected to the main shaft. A lower portion of the liquid feed chamber and a lower portion of the powder feed chamber are separately in communication with an upper portion of a mixing chamber. The mixing chamber is provided therein with an impeller fixedly connected to the main shaft.

A device and apparatus for mixing and dispersing a solid and a liquid are provided. The device includes a powder pulverizing mechanism fixedly connected to a main shaft. The powder pulverizing mechanism is located in a powder feed chamber. The powder feed chamber is provided with a powder feed port. A liquid feed chamber is arranged on an outer side of the powder feed chamber. The liquid feed chamber is provided with a liquid feed port, and the liquid feed chamber is provided therein with a dispersing mechanism fixedly connected to the main shaft. A lower portion of the liquid feed chamber and a lower portion of the powder feed chamber are separately in communication with an upper portion of a mixing chamber. The mixing chamber is provided therein with an impeller fixedly connected to the main shaft.

A fluid path set includes a first fluid line having a proximal end fluidly connectable to a source of a first fluid and a second fluid line having a proximal end fluidly connectable to a source of a second fluid. A flow mixing device is in fluid communication with distal ends of the first and second fluid lines. The flow mixing device includes a housing, a first fluid port provided for receiving the first fluid, and a second fluid port for receiving the second fluid. A mixing chamber is disposed within the housing and is in fluid communication with the first and second fluid ports. A third fluid port in fluid communication with the mixing chamber for discharging a mixed solution of the first and second fluids. A turbulent flow inducing member is disposed within the mixing chamber for promoting turbulent mixing of the first and second fluids.

An impeller assembly for a solid-liquid mixing device, and a solid-liquid mixing device using the impeller assembly. The impeller assembly comprises an impeller. The impeller comprises a truncated conical body. Multiple mixing blades are formed from top to bottom on an inclined surface of the body. The impeller assembly further comprises at least one blocking plate provided on an outer side of a lower portion of the body. The mixing device further comprises: a housing; at least one solid supply apparatus; at least one liquid supply apparatus; a mixing chamber defined by corresponding portions of the body and the housing; and a dispersion chamber provided between a flow channel outlet at a lower portion of the mixing chamber and a discharging apparatus.