A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

A bioreactor system includes a support housing having an interior surface bounding a compartment. A rack is secured to the support housing in alignment with the opening. An elongated first shaft has a first end and an opposing second end, the first end of the first shaft being secured to the rack. A structure is secured to the first shaft at or towards the second end of the first shaft, the structure outwardly projecting away from the first shaft. A collapsible bag bounds a chamber and is at least partially disposed within the compartment of the support housing. A member projects from the collapsible bag and passes through the opening on the support housing, the structure supporting or restraining movement of the member.

A rotary machine for mixing, pumping or agitating a fluid includes an impeller for acting on the fluid, a drive unit for rotating the impeller around a rotation axis, a drive shaft connecting the impeller with the drive unit, a mounting flange for fastening the rotary machine to a wall of a vessel, and a support structure for supporting the rotary machine, the support structure comprising at least one leg, each leg extending in a vertical plane from a first end along a longitudinal axis to a second end, each leg including an outer member and an inner member coaxially arranged in the outer member, and the inner member slidingly movable relative to the outer member for adjusting the length of the respective leg. In addition, a method of mounting a rotary machine for mixing, pumping or agitating a fluid to a wall of a vessel is proposed.

A rotary machine for mixing, pumping or agitating a fluid includes an impeller for acting on the fluid, a drive unit for rotating the impeller around a rotation axis, a drive shaft connecting the impeller with the drive unit, a mounting flange for fastening the rotary machine to a wall of a vessel, and a support structure for supporting the rotary machine, the support structure comprising at least one leg, each leg extending in a vertical plane from a first end along a longitudinal axis to a second end, each leg including an outer member and an inner member coaxially arranged in the outer member, and the inner member slidingly movable relative to the outer member for adjusting the length of the respective leg. In addition, a method of mounting a rotary machine for mixing, pumping or agitating a fluid to a wall of a vessel is proposed.

The present patent application describes a stirrer comprising a combination of at least one axially-conveying element and at least one radially-conveying element relative to the rotary shaft of the stirrer wherein the largest diameter of the at least one axially-conveying element is equal to or less than the inner diameter d.sub.i of the radially-conveying element. In one embodiment the stirrer according to the invention is a combination of one anchor stirrer with at least one inclined-blade stirrer. Furthermore the use of the stirrer according to the invention for the culture of cells in a dialysis method is described.

The present patent application describes a stirrer comprising a combination of at least one axially-conveying element and at least one radially-conveying element relative to the rotary shaft of the stirrer wherein the largest diameter of the at least one axially-conveying element is equal to or less than the inner diameter d.sub.i of the radially-conveying element. In one embodiment the stirrer according to the invention is a combination of one anchor stirrer with at least one inclined-blade stirrer. Furthermore the use of the stirrer according to the invention for the culture of cells in a dialysis method is described.

Disclosed is a foldable agitator. The foldable agitator according to one aspect of the present invention comprises: a coupling body; impeller arms rotatably coupled to the coupling body; and a piston, inserted in the coupling body so as to be movable vertically, for rotating the impeller arms in the process of moving to induce same to fold and unfold.

Disclosed is a foldable agitator. The foldable agitator according to one aspect of the present invention comprises: a coupling body; impeller arms rotatably coupled to the coupling body; and a piston, inserted in the coupling body so as to be movable vertically, for rotating the impeller arms in the process of moving to induce same to fold and unfold.

A fluid mixing system includes a support housing having an interior surface bounding a chamber. A flexible bag is disposed within the chamber of the support housing, the flexible bag having an interior surface bounding a compartment. An impeller is disposed within the chamber of the flexible bag. A drive shaft is coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller. A drive motor assembly is coupled with the draft shaft and is adapted to rotate the drive shaft. An adjustable arm assembly is coupled with the drive motor assembly and is adapted to move the drive motor assembly which in turn moves the position of the drive shaft and impeller. An electrical controller can control movement of the adjustable arm.

A fluid mixing system includes a support housing having an interior surface bounding a chamber. A flexible bag is disposed within the chamber of the support housing, the flexible bag having an interior surface bounding a compartment. An impeller is disposed within the chamber of the flexible bag. A drive shaft is coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller. A drive motor assembly is coupled with the draft shaft and is adapted to rotate the drive shaft. An adjustable arm assembly is coupled with the drive motor assembly and is adapted to move the drive motor assembly which in turn moves the position of the drive shaft and impeller. An electrical controller can control movement of the adjustable arm.