The present invention relates to fluidic devices, especially microfluidic devices, for aliquoting and pairwise combinatorial mixing of a first set of liquids with a second set of liquids. The device architecture is designed to move liquids in two separate phases, a first phase where the liquids are exposed to a first directional force field to move the liquids in a first direction, from a reservoir to aliquot chambers, and a second phase where the liquids are exposed to a second directional force field to move the liquids in a second direction, from the aliquot chambers to the mixing chambers. The first and second directional force fields that the device is exposed to may be achieved using a single directional force field (i.e. a rotor driven centrifugal force field) and by re-orienting the position of the device with respect to the centrifugal forces between the first and second phases of operation. The device architecture comprises reservoirs for each of the first fluids and reservoirs for each of the second fluids. Each reservoir is fluidically connected to aliquoting chambers, either arranged in parallel or in series, for providing aliquots of the fluid which may be metered. The conduits providing fluid communication between the reservoirs and aliquoting chambers are arranged in a first direction. A series of mixing chambers is also provided, and each mixing chamber is fluidically connected to one aliquot chamber for a first liquid and one aliquoting chamber for a second liquid. The conduits providing fluid communication between the aliquoting chambers and mixing chambers are arranged in a second direction.

A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.

The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.

The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.

The invention discloses a multifunctional microfluidic detection chip. The detection chip comprises a chip body, on which a sample injection chamber, a sample quantitative chamber, a sample overflow chamber, a diluent storage chamber, a diluent quantitative chamber, a diluent overflow chamber, a quantitative mixing chamber, a reaction chamber and vent holes are disposed; a sample to be detected is injected into the sample injection chamber, and enters the sample quantitative chamber through a microfluidic channel, and the excess reaction sample enters the sample overflow chamber, a diluent in the diluent storage chamber enters the diluent quantitative chamber through a microfluidic channel, and the excess diluent enters the diluent overflow chamber; the reaction chamber includes one or more reaction cavities and a sample blank cavity; after the sample in the sample quantitative chamber is mixed with the diluent in the diluent quantitative chamber uniformly in the quantitative mixing chamber, mixed liquid enters the reaction cavities through microfluidic channels and reacts with a reaction reagent for detection, and enters the sample blank cavity at the same time as a sample blank for detection. The invention can effectively reduce the sample consumption, improve the accuracy of the detection results, and simultaneously detect multiple indicators.

An exemplary compounding system and device for mixing materials can include a housing, a first material source and a second material source. A first fluid line can be operationally connected to the housing and configured to transport a first volume of fluid per unit time from the first material source to a final container. A second fluid line can be operationally connected to the housing and configured to transport a second volume of fluid per unit time from the second material source to the final container. The device can also include a pump system including, a first pump having a first rotor and a first platen which secures the first fluid line between the first rotor and the first platen, the first pump being configured to move the first volume of fluid through the first fluid line, and a second pump having a second rotor and a second platen which secures the second fluid line between the second rotor and the second platen, the second pump being configured to move the second volume of fluid through the second fluid line. A first platen lock can be provided and can be rotated in a first direction to lock the first platen in a closed position relative to the first rotor, and wherein rotation of the first rotor draws the first material source through the first fluid line. The pump system can also be configured such that the volume of fluid per unit time delivered by the first and second pumps is different, and/or where the first and second pumps have different head characteristics.

The present invention relates to methods, devices, instruments, processes, and systems for the highly specific, targeted molecular analysis of regions of human genomes and transcriptomes from the blood, i.e. from cell free circulating DNA, exosomes, microRNA, IncRNA, circulating tumor cells, or total blood cells. The technology enables highly sensitive identification and enumeration of mutation, expression, copy number, translocation, alternative splicing, and methylation changes using spatial multiplexing and combined nuclease, ligation, polymerase, and sequencing reactions. Such technology may be used for non-invasive early detection of cancer, non-invasive cancer prognosis, and monitoring both treatment efficacy and disease recurrence of cancer.

The presently disclosed subject matter provides devices and methods for sample extraction from a swab during biological sample processing. In particular embodiments, the devices and methods are configured for use in conjunction with microfluidic devices for sample processing.

The invention is an apparatus for mixing and moving small fluid samples including a microfluidic chip with a fluid flow channel and an injection port, a channel light beam with a channel lens configured to converge a channel light beam and project a channel light beam focal spot into the fluid flow channel;

The present invention provides a fluidic device in which solutions of different concentrations can be easily obtained. The fluidic device includes: a first substrate and a second substrate which are stacked in a thickness direction; an undiluted solution introduction flow path which has an undiluted solution introduction port and which is constituted of a groove part provided on at least one of the first substrate and the second substrate; a first circulation flow path which is constituted of a groove part having an annular shape and having a shared part that shares part of a flow path with the undiluted solution introduction flow path and a non-shared part which is not shared with the undiluted solution introduction flow path and which is connected to a diluting solution introduction port; a second circulation flow path which is provided independently of the first circulation flow path and which is constituted of a groove part having an annular shape and having a shared part that shares some flow path with the undiluted solution introduction flow path and a non-shared part which is not shared with the undiluted solution introduction flow path and which is connected to a diluting solution introduction port; and/or a third circulation flow path which is constituted of a groove part having an annular shape and having a shared flow path that shares part of a flow path with the first circulation flow path and a non-shared flow path which is not shared with the first circulation flow path and which is connected to a diluting solution introduction port, wherein the undiluted solution introduction flow path includes a valve at both ends of the shared part.