An electric field stirring apparatus, in which a droplet as a liquid disposed between a first electrode and a second electrode disposed to face each other is vibrated and stirred by an electric field generated between the first electrode and the second electrode, the second electrode having a groove formed along a first direction on an electrode surface facing the first electrode, includes a movement mechanism that reciprocally moves the first electrode relative to the second electrode in a second direction intersecting the first direction in a state in which the first electrode and the second electrode face each other during a period in which the electric field is generated.

The present disclosure provides a microchannel device including a first base having a defining surface that defines a flow channel and containing a polymer that contains a fluorine atom and a second base having a defining surface that defines the flow channel together with the defining surface of the first base, having solvent resistance, and coming into contact with the first base, in which an arithmetic average roughness Ra of a surface of the first base, exposed by peeling the second base from the first base, is 1 μm or more, and provides a use application thereof.

Disclosed herein are systems and methods for serial flow emulsion processes. Systems and methods as described herein result in reduced cross-contamination.

Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

The present invention relates to the use of surfaces that exhibit different surface energies wherein the difference in surface energies is configured to disrupt capillary laminar flow of a fluid travelling between the two surfaces. The invention further relates to the use of such surfaces in assay methods including a device utilising same.

The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.

Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one aspect, the invention relates to systems and methods for making droplets of fluid surrounded by a liquid, using, for example, electric fields, mechanical alterations, the addition of an intervening fluid, etc. In another aspect, the invention relates to systems and methods for dividing a fluidic droplet into two droplets, for example, through charge and/or dipole interactions with an electric field. The invention also relates to systems and methods for fusing droplets, according to another aspect of the invention, for example, through charge and/or dipole interactions. Another aspect of the invention provides the ability to determine droplets, or a component thereof, for example, using fluorescence and/or other optical techniques (e.g., microscopy), or electric sensing techniques such as dielectric sensing.

Devices, systems, and methods for charging fluids are disclosed. The charging of fluids improves the mixing of fluids in microfluidic systems. The charging is performed by producing an ion field between an ionizing electrode and an opposed ground electrode. A fluid-containing vessel is positioned between the opposed electrodes and the ion field charges the fluid in the vessel.

A method and an apparatus for producing various types of microdroplets are provided. The apparatus has a cross intersection portion 7 at which a first continuous phase 2, a first dispersion phase 4, and a second dispersion phase 6 intersect with each other; a first liquid feed device 12 controlling the first dispersion phase 4; a second liquid feed device 13 controlling the second dispersion phase 6; and a control device 11 connected to the first liquid feed device 12 and the second liquid feed device 13, in which the first liquid feed device 12 and the second liquid feed device 13 are controlled by a signal from the control device 11 so that microdroplets 9 formed of the first dispersion phase 4 and microdroplets 10 formed of the second dispersion phase 6 are sequentially produced.

The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.