The invention provides a method for producing prion protein having an aggregated conformation by contacting native conformation prion protein with aggregated conformation prion protein in a liquid preparation and subjecting this to at least one cycle or to a number of cycles of application of shear-force for fragmenting aggregates of prion protein, wherein the shear-force applied is precisely controlled. In addition to this process for amplification of aggregated state prion protein from native conformation prion protein, the invention relates to the aggregated state prion protein obtained by the amplification process, which aggregated state prion protein has one conformation, which is e.g. identical within one batch and reproducible between batches, e.g. as detectable by proteinase resistance in a Western blot.

A packaging for a flexible container, in particular for a disposable bag for use in a bioreactor, comprises a bottom part for fixing a first portion of the container, a ceiling part for fixing an opposite second portion of the container, a receiving space which on a first side is defined by the bottom part and on an opposite second side is defined by the ceiling part, and struts arranged between the bottom part and the ceiling part, which fix a defined distance between the bottom part and the ceiling part.

This disclosure relates to a drink container with lid for the mixing and blending of liquids for consumption, wherein the unique design of the drink container tends to improve the texture and flavor of the liquid blended within it.

What is proposed is an emulsifying system with an emulsifying device and an injection nozzle as well as an emulsifying device for producing a water-fuel emulsion for an internal combustion engine, wherein the emulsifying device is embodied as a rotor-stator emulsifying device and/or fluid flow machine and/or is connected or connectable directly to an injection nozzle. The emulsifying device has a housing and a shaft, the shaft being drivable in a contactless manner, the housing having a guide apparatus having a plurality of guide channels for guiding the flow, and/or the housing being made at least partially from fiber composite material. Moreover, an emulsifying method for producing a water-fuel emulsion is proposed in which water and fuel are fed to a rotor-stator emulsifying device and/or fluid flow machine for producing the water-fuel emulsion, and/or in which water and fuel are premixed in a first emulsifying stage and fed via a guide apparatus having a plurality of guide channels to a second emulsifying stage.

A multi-level port vessel includes a vessel body defining an interior volume. The vessel body has a front wall, a rear wall, two side walls, a bottom, and a top. The top is open and adapted to be covered by a removable lid. A vertically oriented mixing wheel is disposed within the interior volume. The vertically oriented mixing wheel has a substantially horizontal rotation axis when the vessel body is disposed on a substantially horizontal surface. A plurality of ports is disposed in one of the rear wall, the front wall, or the side walls. At least one port in the plurality of ports is located at an elevation that is at least partially encompassed by a projection of the perimeter of the vertically oriented mixing wheel.

Disclosed is a method for assembling a mixer-container intended for receiving a biopharmaceutical fluid in which: a container is provided, the container including: a mixing device including at least one shaft having an adjustable length, and at least one first bearing attached to the wall, the shaft extending at least into the inner space from the first bearing; a rigid outer device that compresses the container is provided; a drive motor located outside the container is provided; the first bearing of the container is placed so as to be spaced apart from the motor; the length of the shaft is adjusted along the main axis by arranging the shaft opposite the motor to enable the motor to rotate the shaft.

A magnetic coupling assembly for a magnetic stirrer includes a non-magnetic disc embedded with two or more pairs of magnets. The two magnets of each pair are oriented with opposite magnetic poles pointing in the upward direction. When the magnetic coupling assembly is driven to rotate, it can bind one or more axially magnetized stir bar in a mixer at a relatively higher speed to achieve better mixing result without causing spin-out or jumping-flea phenomena, compared to when only one pair of magnets is used.

The invention relates to a mixing system, in particular a bioreactor and/or a pallet tank, for mixing a fluid and/or solid, having a container (4), wherein the fluid and/or the solid and a rotatable stirring element (3) for mixing the fluid and/or the solid are arranged in the interior of the container (4). The mixing system furthermore has a mixing device (1) for receiving the container (4) and a drive device (2) for driving the stirring element (3). The drive device (2) comprises a stator (20) of a three-phase machine (10; 11), the stirring element (3) comprises a rotor (30) of the three-phase machine (10; 11), and the rotor (30) has at least one permanent magnet (31; 31) and/or at least one short circuit rotor.

Methods for sampling reactor contents in parallel reactor systems are disclosed. The methods may be used to sample reactor contents in non-atmospheric (e.g., pressurized) reaction vessels.

A mixing device having a stirring element can include: a container for receiving fluids and/or solids; and at least one rotatable stirring element for mixing the fluids and/or solids; wherein the stirring element comprises a first bearing element and a second bearing element which are arranged at or near opposite ends of the stirring element; wherein the first bearing element is mounted on a first face of the container and the second bearing element is mounted on an opposite second face of the container; wherein the first bearing element comprises at least one non-permanently magnetized element such that it can be moved in rotation by externally induced reluctance forces, and wherein the second bearing element is mounted in a contactless manner by externally induced magnetic forces.