The present invention relates to methods, devices, instruments, processes, and systems for the highly specific, targeted molecular analysis of regions of human genomes and transcriptomes from the blood, i.e. from cell free circulating DNA, exosomes, microRNA, IncRNA, circulating tumor cells, or total blood cells. The technology enables highly sensitive identification and enumeration of mutation, expression, copy number, translocation, alternative splicing, and methylation changes using spatial multiplexing and combined nuclease, ligation, polymerase, and sequencing reactions. Such technology may be used for non-invasive early detection of cancer, non-invasive cancer prognosis, and monitoring both treatment efficacy and disease recurrence of cancer.

An all-in-one mobile wellsite service unit for providing equipment and services at an oil and gas well related to well abandonment is provided. The mobile unit comprises a water storage tank, at least one cement mixing barrel for mixing a cement slurry, a progressive cavity pump for pumping the cement slurry, and a hydraulic hose for connecting to a hydraulic power source to provide power to the cement mixing barrel and the progressive cavity pump. The mobile unit may also contain downhole tools needed for well abandonment, including a well cleaning tool, a cementing tool, a hydraulic packer, and more.

A system for creating an oxidation reduction potential (ORP) in water employs a plurality of ozone supply units housed in separate enclosures. The ozone supply units feed into a manifold that contains a plurality of fluid paths and has one or more ozone intake ports. The ozone intake ports are fluidically coupled to one or more ozone output ports of each ozone supply unit. The manifold includes a plurality of flow switches configured to transmit control signals to one or more controllers of each ozone supply unit in response to sensing a flow of water through the fluid paths in order to cause the ozone supply units to generate ozone. The manifold also includes a plurality of fluid mixers that are fluidically coupled to the ozone intake ports and configured to introduce the ozone generated by the ozone supply units into the water flowing through the fluid paths.

Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.

The present invention relates to a microfluidic device, a method for manufacturing a microfluidic device, and a method for provision of double emulsion droplets using a microfluidic device. Furthermore, the present invention relates to an assembly configured to supply pressure to the microfluidic device for provision of double emulsion droplets. Furthermore, the present invention relates to a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the microfluidic device for provision of double emulsion droplets. The microfluidic device comprises a transfer conduit comprising a first transfer conduit part having a first affinity for water; and a collection conduit comprising a first collection conduit part having a second affinity for water being different from the first affinity for water. A well section and a microfluidic section of the microfluidic device are fixedly connected to each other.

An apparatus, vortex generator assembly and method for automated cell lysis and nucleic acid purification and processing. The vortex generator assembly includes sample holder having a lysis well, at least one wash well, and an elution well. The vortex generator assembly also includes a sample holder cover having a plurality of vibration rods for creating a vortex in the wells of the sample holder. The apparatus includes motor operating a rotating cam to cause the vibration rods to vibrate and create the vortex in a well holding fluid and magnetic beads, wherein the vortexing speed is sufficient to overcome the magnetic attraction between the beads and disperse the beads in solution, to collect nucleic acids such as DNA.

Methods of manufacturing a substrate including a component, such as superabsorbent material, having a volatile hydrophobic coating are disclosed. The method can include providing a fluid supply including a liquid and providing a supply of the component. The component can include a volatile hydrophobic coating. The method can include introducing the component to the fluid supply. The method can also include transferring the component in the fluid supply to provide the substrate. The method can further include applying heat to the substrate. The heat can remove the volatile hydrophobic coating from the component.

One aspect of the present disclosure is an apparatus for manufacturing cosmetic containing: a main body providing a space for manufacturing cosmetic; a mixing vessel that can store cosmetic materials and a parts supply unit providing components including a plurality of cosmetic containers; a cosmetic material supply unit that can store cosmetic materials and then discharge cosmetic materials to the mixing vessel; a transfer unit that can transfer the mixing vessel and the components including the cosmetic container; and a control unit that can control the movement of the transfer unit in order to simultaneously assemble a plurality of cosmetic containers from the mixing vessel and the components.

In a fluid injection system for dispensing a solution, said fluid injection system comprises a feeder tank having a product to be dispensed therein, an inlet connection for diverting fluid from a flow line to a fluid nozzle means and an outlet connection for dispensing the solution into the flow line or onto a matter. The fluid nozzle means is in communication with the feeder tank and the inlet connection and the fluid injection system controls a flow rate of fluid at which the fluid nozzle means introduce the fluid into the feeder tank based upon characteristics comprising solubility of the product at a temperature of the fluid in the feeder tank, weight of the product to be dispensed or to be dissolved in the feeder tank, and dispensing time, and the fluid injection system controls a flow rate of the fluid nozzle means to satisfy equation: F=(W)/(T×S) wherein, F is the flow rate of the fluid nozzle means, W is weight of the product in the feeder tank, T is dispensing time and S is solubility of the product at the temperature of the fluid in the feeder tank.

A microfluidic device contains a first layer having a plurality of channels, a second layer having a plurality of droplet makers, and a third layer having a plurality of through-holes connecting the plurality of channels to the plurality of droplet makers. The channels have a height of at least 4 times greater than the height of the droplet makers. The microfluidic device has at least 500 droplet makers in an area less than 10 cm.sup.2. The channels are formed by direct laser-micromachining and the droplet makers are formed by soft lithography molding.