A microfluidic device includes at least one substrate formed of one or more silicon wafers. The substrate includes an inlet for receiving a continuous phase fluid; an inlet for receiving a dispersed phase fluid; and a plurality of channels. The plurality of channels are in fluid communication with both the inlet of the continuous phase fluid and the inlet of the dispersed phase fluid. The substrate further includes a plurality of droplet generators configured to produce microdroplets. Each of the droplet generators are in fluid communication with the plurality of channels. Additionally, the substrate includes one or more outlets for delivery of the microdroplets. The number of the plurality of droplet generators is more than two greater than a number of the one or more outlets for delivery of the microdroplets.

A concentrating-enriching magnetic bead purifier includes a base, at least one first mixing sleeve socket, at least one second mixing sleeve socket, and a plurality of wells, and a controlling module. The first mixing sleeve socket includes a first magnetic rod, and a first magnetic rod sleeve. The second mixing sleeve socket includes a second magnetic rod and a second magnetic rod sleeve. A diameter of a cross section of the second magnetic rod sleeve is smaller than that of the first magnetic rod sleeve. The wells include at least one binding well, at least one washing well, and at least one elution well. The volume of the elution well is smaller than that of the binding well. The controlling module controls the first magnetic rod sleeve to only mix or adsorb in the binding well and the washing well and controls the second magnetic rod sleeve to mix or adsorb in the washing well and the elution well.

Provided is a carbon dioxide fluidity control device comprising, a sample preparation tank, a high-pressure stirring unit, a reciprocating plunger pump and a booster pump, wherein the stirring unit comprises one or more high-pressure stirring tanks, each provided with an atomizing spray probe and a piston, wherein a discharge port of the sample preparation tank is connected to the atomizing spray probe via a plunger pump, which is connected to the piston to push the piston to reciprocate; the booster pump is connected to the high-pressure stirring tanks to provide supercritical carbon dioxide to the high-pressure stirring tank; and a discharge port of the high-pressure stirring tanks is connected to an oilfield well group. Provided is a carbon dioxide fluidity control method using the device, comprising mixing surfactants and nanoparticles with heated carbon dioxide, and injecting a microemulsion of supercritical carbon dioxide and nano-silicon dioxide into an oilfield well group.

The invention relates to a modular sterilizable system for organoid culture, which comprises a culture module with one or more sample wells and an stirring module that includes an air compressor with flow control means, a nozzle for each sample well, a pressure sensor and a controller that acts on the flow control means according to the pressure reading. The method for sterilizing the system consists in decoupling the two modules, removing the culture module and sterilizing it. The system can have a module for monitoring the growth of the organoids, as well as a module for controlling the physiochemical parameters of the culture.

A system for creating an oxidation reduction potential (ORP) in water employs a manifold. The manifold includes an enclosure containing a plurality of fluid paths and having one or more ozone intake ports. The ozone intake ports are fluidically coupled to one or more ozone output ports of an ozone supply unit housed in a separate enclosure. A plurality of flow switches are disposed within the enclosure and configured to transmit control signals to one or more controllers of the ozone supply unit in response to sensing a flow of water through the fluid paths in order to cause the ozone supply unit to generate ozone. A plurality of fluid mixers are also disposed within the enclosure. The fluid mixers are fluidically coupled to the ozone intake ports and configured to introduce the ozone generated by the ozone supply unit into the water flowing through the fluid paths.

A system for creating an oxidation reduction potential (ORP) in water employs a plurality of ozone supply units housed in separate enclosures. The ozone supply units feed into a manifold that contains a plurality of fluid paths and has one or more ozone intake ports. The ozone intake ports are fluidically coupled to one or more ozone output ports of each ozone supply unit. The manifold includes a plurality of flow switches configured to transmit control signals to one or more controllers of each ozone supply unit in response to sensing a flow of water through the fluid paths in order to cause the ozone supply units to generate ozone. The manifold also includes a plurality of fluid mixers that are fluidically coupled to the ozone intake ports and configured to introduce the ozone generated by the ozone supply units into the water flowing through the fluid paths.

Method of detecting a target nucleic acid. In an exemplary method, at least two thermal zones of different temperature may be created using a heating assembly. A first emulsion and a second emulsion may be formed. The first and second emulsions may be thermally cycled by passing them through tubing in a spaced relation to one another, with the tubing being wound around a central axis of the heating assembly and extending through each thermal zone multiple times. Thermally cycling may promote amplification of the target nucleic acid in droplets of each emulsion. Droplets of each emulsion may be passed through a detection channel located downstream of the tubing. Fluorescence may be detected from the droplets being passed through the detection channel.

A system for distributing ozonated fluid includes a manifold that contains a plurality of fluid paths and has one or more ozone intake ports. The ozone intake ports are fluidically coupled to one or more ozone output ports of one or more ozone supply units. The manifold includes a plurality of flow switches configured to transmit control signals to one or more controllers of each ozone supply unit in response to sensing a flow of water through the fluid paths in order to cause the ozone supply units to generate ozone. The manifold also includes a plurality of fluid mixers that are fluidically coupled to the ozone intake ports and configured to introduce the ozone generated by the ozone supply units into the water flowing through the fluid paths.

An apparatus (2) for dissolving a gas into a liquid includes a liquid inlet (4) for supplying liquid into the apparatus, a gas inlet (6) for supplying gas into the liquid within the apparatus and a venturi (52) arranged to dissolve the gas into the liquid passing through the venturi. The apparatus also includes an outlet (18) for the liquid and dissolved gas downstream of the venturi. At least part of the liquid inlet, at least part of the gas inlet, at least part of the venturi and at least part of the outlet are formed in an integrally formed piece of material (42).

The present disclosure is directed towards improved systems and methods for large-scale production of nanoparticles used for delivery of therapeutic material. The apparatus can be used to manufacture a wide array of nanoparticles containing therapeutic material including, but not limited to, lipid nanoparticles and polymer nanoparticles. In certain embodiments, continuous flow operation and parallelization of microfluidic mixers contribute to increased nanoparticle production volume.