A centrifugal microfluidic device having a microfluidic mixing element with a microfluidic mixing chamber in which at least two flows emerging from channels into the chamber at separate places are redirected to land at substantially the same place on a mixing surface provides efficient mixing of two or more fluids in the chamber.

Accordingly, in some embodiments of the disclosure, a multi-chambered assay device is provided, which is configured for arrangement on a disc, as well as configured to process an individual sample. A plurality of such assay devices can be arranged along a periphery of the disc at a distance/radius from the center, such that a plurality of individual samples can be processed, e.g., one per assay device. In addition, in an arrangement that a plurality of assay devices are used, they can be spaced apart such that they balance the disc during rotation (which can be with samples contained in one or more of the assay devices, a plurality, a majority, or all of the assay devices).