The invention relates to an apparatus for analyzing reaction systems with a liquid phase (13) and a gas phase (15), the apparatus (1) comprising at least two tank reactors (3), a common feed line (5), a common drain line (25) for the liquid phase and a common drain line (21) for the gas phase, each tank reactor (3) being connected to the common feed line (5) by a supply line (7), to the common drain line (25) for the liquid phase by a liquid withdrawal line (27) and to the common drain line (21) for the gas phase by a gas withdrawal line (23), wherein the pressure in each tank reactor (3) is controlled by one of: (a) a pressure control (31) in the common feed line (5); (b) a pressure line (29) which is connected to the gas space of each tank reactor (3); (c) a pressure control (31) in the common drain line (21) for the gas phase and a flow restrictor (33) in the common drain line (25) for the liquid phase or a pressure control (31) in the common drain line (25) for the liquid phase and a flow restrictor (33) in the common drain line (21) for the gas phase; or (d) a pressure line (29) which enters into the common drain line (25) for the liquid phase or into the common drain line (21) for the gas phase.

The invention further relates to a process for analyzing reaction systems in such an apparatus.

A drug product reconstitution processing system includes a drug vial tray having a plurality of walls, a first robotic arm movable between a plurality of positions above the drug vial tray, and a second robotic arm movable between a plurality of positions above the drug vial tray. The first robotic arm includes a drug vial transfer system adapted to retrieve a drug vial disposed within one of the plurality of wells and a vial agitation system adapted to agitate the drug product contained within the drug vial. The second robotic arm includes a drug vial reconstitution system adapted to selectively add a fluid to the drug vial and/or remove a fluid from the drug vial.

A parallel multi-step bio-reaction system(10) comprising: (a) a substrate arrangement(12) comprising a plurality of bio-reaction substrate holders(18) configured to hold a plurality of bio-reaction substrates(20); (b) a well arrangement(14) comprising a plurality of fluidic wells(22), the fluidic wells(22) corresponding to a plurality of steps of a multi-step bio-reaction; (c) an actuator(16) configured to: (i) move either the substrate arrangement(12) or the well arrangement(14) relative to the other of the substrate arrangement(12) or the well arrangement(14) to change the alignment of the bio-reaction substrates(20) as a group relative to the fluidic wells(22) as a group; and (ii) bring the bio-reaction substrates(20) into and out of contact with fluids in the fluidic wells(22).

The present invention is directed to a microfluidic system comprising a microfluidic chip and a method of performing a chemical assay wherein a sample is processed into multiple daughter droplets and said daughter droplets are incubated with varying reagents. The properties of these droplets can be detected to provide assay data.

Embodiments in accordance with the present disclosure are directed to apparatuses used for reaction screening and optimization purposes. An example apparatus includes a plurality of reaction vessels, a dispensing subsystem, at least one reactor module, an analysis subsystem, an automation subsystem, and control circuitry. The dispensing subsystem delivers reagents to the plurality of reaction vessels for a plurality of reaction mixtures having varied reaction conditions. The at least one reactor module drives a plurality of reactions within the plurality of reaction vessels. The analysis subsystem analyzes compositions contained in the plurality of reaction vessels. The automation subsystem selectively moves the plurality of reaction vessels from a location proximal to the dispensing subsystem to the at least one reactor module based on experimental design parameters. And, the control circuitry identifies optimum reaction conditions for a target end product based on the analysis.

A chemical solution synthesis device comprises a chemical solution holding unit, a reaction vessel, a first chemical solution feed unit, a chemical solution feed and discharge unit, and a second chemical solution feed unit. The chemical solution holding unit is configured to hold a chemical solution. The chemical solution and carriers are reacted in the reaction vessel. The chemical solution is transferable from the chemical solution holding unit to the reaction vessel without coming into contact with atmosphere. The first chemical solution feed unit is connected to the reaction vessel and configured to supply the chemical solution to the reaction vessel. The chemical solution feed and discharge unit is provided vertically above the first chemical solution feed unit. The second chemical solution feed unit is configured to suppress adhesion of the carriers to the reaction vessel.

Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 l). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.

According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.

An apparatus for optically-verified de novo DNA synthesis includes a microfluidic system that has channels leading in and out of a synthesis chamber having a functionalized region on a floor thereof on which a single-strand of DNA to which a nucleotide is to be attached can be fixed. The chamber is in optical communication with both an illumination system, which excites an electron in a fluorophore that is attached to the DNA strand, a detection system, which detects a signature photon emitted as the excited electron decays into its ground state.

Library preparation systems and methods are disclosed. In an implementation, a modular bay for preparing a library of samples for sequencing, the modular bay comprising a contact dispenser and a working area comprising a working plate receptacle adapted to receive a working plate, a thermocycler, a magnet, and a drawer. The drawer comprising a consumables area adapted to receive a sample plate adapted to contain a sample, and a plurality of consumables for interacting with the sample in the working plate. The contact dispenser is linearly movable in a longitudinal direction between the consumables area and the working area, such that the contact dispenser is configured to (i) move the sample from the sample plate in the consumables area to the working plate in the working area, and (ii) move the plurality of consumables between the consumables area and the working plate in the working area.