System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.

Syngas is alternatingly introduced by a syngas alternating lead-in system through either of one- and the other-end-side heat storage bodies into flow passages in a primary reforming furnace, and oxidant is alternatingly supplied to the syngas by a primary-oxidant alternating supply system. The syngas derived from the primary reforming furnace by a syngas alternating lead-out system is introduced into a secondary reforming furnace to which connected is a secondary-oxidant supply system for supply of oxidant only at alternation in the syngas alternating lead-in and -out systems.

Disclosed is a technology based upon the nesting of tubes to provide chemical reactors or chemical reactors with built in heat exchanger. As a chemical reactor, the technology provides the ability to manage the temperature within a process flow for improved performance, control the location of reactions for corrosion control, or implement multiple process steps within the same piece of equipment. As a chemical reactor with built in heat exchanger, the technology can provide large surface areas per unit volume and large heat transfer coefficients. The technology can recover the thermal energy from the product flow to heat the reactant flow to the reactant temperature, significantly reducing the energy needs for accomplishment of a process.

A method for processing a biological material sample includes dispensing a sample into wells of an array tape from a sample plate, dispensing a reagent into the wells of the array tape from a reagent plate, and sealing the sample and the reagent in the array tape. The method further includes cooling the array tape and detecting biological material in the wells of the array tape.

A digital PCR device comprising: a discrete heating area made of a heat conductive material disposed on a surface that is electrically non-conductive, the discrete heating area comprising a plurality of integral wells configured to contain and partition a DNA sample therein; and at least one conductive trace configured to be connected to a dc voltage source and to heat the plurality of integral wells to a uniform temperature when connected to the dc voltage source, the at least one conductive trace disposed on the surface in an undulating configuration at least partially around the discrete heating area.

A sequencer pretreatment device includes a suction and discharge mechanism, a nozzle head having a nozzle for mounting a dispensing tip, a container group for accommodating liquids including magnetic particle suspension, a moving mechanism for causing relative movement between the nozzle and the container group, and a magnetic unit that exerts a magnetic field to the mounted dispensing tip. A method includes an extraction step of mixing a sample, extraction reagent solution, and magnetic particle suspension in the container group and extracting nucleic acid, a fragmentation producing step of fragmentating the nucleic acid by mixing with fragmentation solution accommodated in the container group and producing a fragment of a base sequence having a molecular weight corresponding to a sequencer using magnetic particle suspension using the sequencer pretreatment device, and an amplification pretreatment step of dispensing a solution containing the fragment into the reaction vessel using the sequencer pretreatment device.

A device (100) and a method optically control a chemical reaction in a reaction chamber (149) holding a reagent fluid (114). The chemical reaction includes a nucleic acid sequencing on a wiregrid. Based on strong optical confinement of excitation light (110) and of cleavage light (112), the sequencing reaction can be read-out. Stepwise sequencing is achieved by using nucleotides with optically cleavable blocking moieties. After read-out the built in nucleotide is deblocked by cleavage light through the same substrate. This ensures that only bound nucleotides will be unblocked. In order to avoid overheating by cleavage light, the reagent fluid is circulated along the surface of the substrate (101).

A sensor array includes a semiconductor substrate, a first plurality of FET sensors and a second plurality of FET sensors. Each of the FET sensors includes a channel region between a source and a drain region in the semiconductor substrate and underlying a gate structure disposed on a first side of the channel region, and a dielectric layer disposed on a second side of the channel region opposite from the first side of the channel region. A first plurality of capture reagents is coupled to the dielectric layer over the channel region of the first plurality of FET sensors, and a second plurality of capture reagents is coupled to the dielectric layer over the channel region of the second plurality of FET sensors. The second plurality of capture reagents is different from the first plurality of capture reagents.

An integrated circuit includes two or more rows of heating elements, two or more columns of heating elements, and a plurality of sensing areas. Each sensing area is between two adjacent rows of the rows of heating elements and between two adjacent columns of the columns of heating elements and includes a bio-sensing device and a temperature-sensing device.

An integrated circuit includes two or more rows of heating elements, two or more columns of heating elements, and a plurality of sensing circuits. Each sensing circuit is between two adjacent rows of the rows of heating elements and between two adjacent columns of the columns of heating elements, in a same silicon layer as the rows of heating elements and the columns of heating elements, and configured to generate a bio-sensing signal and a temperature-sensing signal.