A microarray analysis method, in which a microarray obtained by arranging probes on a substrate surface having an irregular shape is irradiated with excitation light and fluorescence amounts of the probes excited by the excitation light are obtained as numerical data, includes a step (a) of measuring the fluorescence amounts of the probes to acquire fluorescence image data, a step (b) of receiving reflected light and/or scattered light from the substrate surface to acquire the irregular shape of the substrate surface of the microarray as alignment image data based on the light receiving intensities of the light, and a step (c) of determining positions of the probes on the fluorescence image data based on the alignment image data.

Devices and methods are provided for spotting an array with fluid. Arrays produced by such methods are also provided. In one aspect of the invention, a spotter device for spotting a plurality of fluids into an array is described, the spotter device comprising a plurality of reservoirs provided in a first configuration, each reservoir holding its respective fluid, a print head having a plurality of positions provided in a second configuration, the second configuration being different from the first configuration, a plurality of tubes, each tube configured to provide fluid communication from a reservoir at a first end of the tube to a position in the print head at the second end of the tube, and a pump for pumping fluid through the tubes from the reservoir to the print head.

The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications.

Reagents and solvents used for polymer synthesis are reused or recycled rather than discarded. The outflow from each step of polymer synthesis may be collected separately in one of multiple dedicated containers. Reuse returns the outflow from a step of polymer synthesis back to an input of a polymer synthesizer for subsequent use in that same step. Recycling processes the outflow from one or more steps of polymer synthesis to restore original concentrations or purity levels for use in a later synthesis run. Quality control analysis may determine if outflow collected from a polymer synthesizer is reused or recycled. These techniques reduce reagent cost and waste quantity. These techniques may be used with phosphoramidite or enzyme-based synthesis of deoxyribonucleic acid (DNA).

The present invention relates to a method and device for manufacturing microarrays, wherein a microarray comprises a plurality of spots, for testing the interaction of biomolecules. Disclosed herein is a method for enhancing efficiency of overlay printing of spot positions on multiple slides or plates arranged in an array wherein a slide or plate order is provided by rows and columns.

The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications.

The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites. In particular, the methods, compositions, and systems of the present invention result in loading of polymerase enzyme complexes into a predetermined number of reaction sites, including nanoscale wells.

A modular reactor device has an outer housing, a reaction chamber, a fluid pathway connected to the reaction chamber, and a valve to control flow of fluid within the device. The outer housing has a plurality of connection ports including: a fluid input and a fluid output; an electrical input; and a pneumatic input. Either the electrical input or the pneumatic input is connected to the valve to provide for control of the valve, and either the fluid input or the fluid output is connected to the reaction chamber or the fluid pathway. Other aspects provide a base station for receiving and controlling a modular reactor device and methods for manufacturing the modular reactor device and for performing reactions using a modular reactor device.

A prefabricated multi-test kit, the kit that comprises a plurality of reaction cells, each one of at least two of the reaction in cells holding reagents of a respective, different set of at least two tests selected from a group consisting of a plurality of different tests, the tests being distributed among the reaction cells in a manner that leaves reagents of each different one of the tests of the group in a respective different sub-combination of the reaction cells, and allows any sub-combination of the reaction cells to be indicative of a sub-group comprising all positive tests of the group when each reaction cell of the sub-combination that is indicative of the sub-group, contains an at least one positive test and none of the remaining reaction cells contain a positive test.

A microarrayer for dispensing reagent onto a substrate, comprising a dispensing print head adapted to load reagent and provided with a plurality of nozzles to dispense said at least reagent on the substrate, wherein the print head is mounted in the microarrayer to allow the print head to move with respect to the substrate in subsequent, essentially, parallel print passes, wherein the print head moves during a first print pass between a first end of the substrate toward a second end of the substrate and during a subsequent printpass in the opposite direction, the microarrayer being characterised in that the microarrayer comprises a first camera adapted to move behind the print head during a print pass and a second camera adapted to move ahead of the print head during said print pass, the first and second camera being adapted to obtain images of the substrate.