Systems, methods, and compositions for high throughput screening of micro-scale chemical reactions are disclosed. In particular, systems, methods, and compositions for handling small amounts of solid reagent are disclosed. For example, mechanical mixing is employed to obtain reagent-coated bulking agents that can be used, inter alia, in high throughput methods for screening micro-scale chemical reactions.

A method includes forming a composition of matter including an amount of a reporter compound, a binding member capable of capturing the reporter compound, and an amount of a target nucleic acid capable of forming complexes with the reporter compound.

The present invention provides a system for performing reactions, where the system comprises a plurality of synthesisers that are in communication via a communal reporting platform. A synthesiser is programmed for the automated synthesis of one or more chemical or biological reactions, and the synthesiser comprises a reaction vessel which is supplied by a reagent delivery system, an analytical system for analysing a reaction, and a controller for managing the reagent delivery system and the analytical system, and for communication with the reporting platform. Also provided are methods for performing a plurality or reactions using the system.

Methods and compositions for manufacturing large-scale quantities of conjugatable peptides/peptoids/polymers/nucleic acids and conjugatable proteins, as well as hybrid materials consisting of synthetic and unnatural amino acids, glycopeptides, proteoglycans, and other molecular modifications are disclosed, for a variety of purposes including rapid antidote and vaccine applications in biodefense, therapeutics, diagnostics, theranostics, thin films, multilayered assemblies, biofilms, sensors, drug delivery vehicles, gene delivery vehicles, gene editing vehicles, staged release compounds, and the like.

The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.

The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

The invention relates to an apparatus for producing pulverulent poly(meth)acrylate, comprising a reactor for droplet polymerization having an apparatus for dropletization of a monomer solution for the preparation of the poly(meth)acrylate having holes through which the monomer solution is introduced, an addition point for a gas above the apparatus for dropletization, at least one gas withdrawal point on the circumference of the reactor, a fluidized bed and an apparatus for product discharge from the fluidized bed. The apparatus for product discharge comprises a discharge apparatus, with a backup segment (39) disposed above the discharge apparatus.

Systems, methods, and devices for generating radionuclides for use in production of radiopharmaceuticals; synthesizing the radionuclides generated and removing any unwanted products; measuring the quantity and activity level of the synthesized radionuclides; distributively delivering the radionuclides in appropriate quantities to modular cassette synthesis units in a modular cassette subsystem for contemporaneous/parallel production of radiopharmaceutical output and that allow reuse and/or quick, safe, and disposable replacement of portions of the subsystem; delivering non-radionuclide components to the modular cassette synthesis units as part of production of radiopharmaceutical output; measuring the quantity and activity level of each stream of radiopharmaceutical output; purifying the radiopharmaceutical output; dispensing individual doses in sterile vial(s); automatically producing labeling and dose related information; performing automated quality control on extracted samples of produced radiopharmaceutical output; and providing software and hardware controls for overall and sub-portion operation for optional remote data collection, communication, and/or control.

A reactive distillation method comprises introducing a feed stream to a reactive distillation column, wherein the feed stream comprises ethanol, contacting the feed stream with one or more catalysts in the reactive distillation column during a distillation, and removing butyl acetate during the distillation from the reactive distillation column as a bottoms stream. The feed stream reacts in the presence of the one or more catalysts to produce a reaction product comprising ethyl acetate, butanol, and water, wherein the butanol and the ethyl acetate react to produce a reaction product comprising the butyl acetate.