There is provided a microchip. The microchip comprises a substrate including a flow channel configured to convey a fluid therein. The substrate comprises a first substrate layer, a second substrate layer laminated to the first substrate layer to create the flow channel, and a discharge part formed in only one of the first substrate layer or the second substrate layer. The discharge part includes an opening directed toward an end face of the substrate, and being configured to eject the fluid flowing through the flow channel.

An insert for a micro-nozzle, and a micro-nozzle comprising such insert. The insert comprises a microfabricated fluidic chip having an inlet, an outlet and one or more microfluidic channels connecting the inlet and outlet and an overmould casing overmoulded around the fluidic chip so as to substantially encase the fluidic chip, and comprising an inlet in fluid communication with the inlet of the chip and an outlet in fluid communication with the outlet of the chip.

Compositions and methods for identifying antigen-specific T cells, including determining paired T cell receptor sequences for a specific antigen, are described. Compositions and methods for identifying neoantigen-specific T cells are also described. Microfluidic devices useful for identifying antigen-specific T cells, and methods of using the same, are also described.

(Object) To improve the accuracy of the placement of liquid droplets. (Means of Achieving the object) A liquid droplet discharging method is performed by a liquid droplet discharging apparatus configured to discharge a liquid droplet from a nozzle hole formed in a film-like member, the liquid droplet discharging method including positioning the nozzle hole inside a recessed portion provided in a container; and discharging the liquid droplet from the nozzle hole positioned inside the recessed portion.

In one example in accordance with the present disclosure, a fluid ejection controller is described. The fluid ejection controller includes a firing board to pass control signals to a fluid ejection device to eject fluid from the fluid ejection device. A mount pivotally holds the firing board between a disengaged position where electrical pins of the firing board are not in contact with electrical pads of the fluid ejection device and an engaged position where the electrical pins are in contact with the electrical pads. The mount includes a slot to receive the fluid ejection device and at least one biasing spring to bias the firing board away from the fluid ejection device during insertion of the fluid ejection device. The fluid ejection controller also includes a handle coupled to a cam shaft to move the firing board between the disengaged position and the engaged position.

An apparatus including a fluidic input and a die including a microfluidic chamber, may receive a biologic sample. The microfluidic chamber may include a foyer to contain a portion of the biologic sample, and an inlet impedance-based sensor to detect passage of a cell of the biologic sample into the foyer. A target nozzle may eject a first volume, corresponding with a target concentration of cells of the biologic sample. A spittoon nozzle may eject a second volume of the portion of the biologic sample into a spittoon location. An output impedance-based sensor may be disposed within a threshold distance of the target nozzle to detect passage of a cell of the biologic sample into the target nozzle. Moreover, the apparatus may include circuitry to control firing of the target nozzle and the spittoon nozzle based on signals received from the inlet impedance-based sensor and the output impedance-based sensor.

Disclosed are a droplet generation method, system and application thereof. The method breaks through the limitation that the existing nanoliter scale droplet generation technology must use micro-channels below 0.1 mm, and can realize the preparation of small-volume uniform droplets at a reduced cost. The system includes a droplet generation device and a droplet receiver, the droplet generation device includes an accommodating cavity with a variable volume, a control mechanism for controlling the volume of the accommodating cavity to change periodically, and a droplet generation tube, which has a wide range of applications in clinical diagnosis, gene expression analysis, microorganism detection and other fields.

In one example in accordance with the present disclosure, a fluidic ejection controller is described. The fluidic ejection controller includes a firing board to pass electrical control signals for ejecting fluid from a fluidic ejection device. An ejection board of the fluidic ejection controller is electrically coupled to, and selectively removable from, the firing board to pass the electrical control signals to the fluidic ejection device. Electrical pins are disposed on the ejection board in a pattern that matches a pattern of electrical pads on the fluidic ejection device. The electrical pins interface with corresponding electrical pads to pass the electrical control signals from the ejection board to the fluidic ejection device.

A system and method for printing cells in a medium. A multi-dimensional printer, stably constructed of low-mass parts, can include a computer numerically controlled system that can enable motors driving delivery systems. The motors can include encoders that can enable achieving arbitrary resolution. The motors can drive ballscrews to enable linear motion of delivery systems, and the delivery systems can enable printing of a biological material in a pre-selected pattern in a petri dish. The petri dish can accommodate a medium such as a gel, and can further accommodate a vision system that can detect actual position and deflection of the delivery system needle. The printer can accommodate multiple delivery systems and therefore multiple needles of various sizes.

Focused acoustic radiation, referred to as tonebursts, are applied to a volume of liquid to generate a set of droplets. In one embodiment, a first toneburst is applied to temporarily raise a mound or protuberance on a free surface of the fluid. After the mound has reached a certain state, at least two additional toneburst can be applied to the protuberance to sequentially eject multiple bursts of multiple droplets. In one embodiment, the state of the mound can be maintained by a sustained acoustic signal, during which time multiple additional tonebursts can be applied to sequentially eject multiple bursts of multiple droplets from the mound.