A method for forming discrete volumes of aqueous fluid may comprise flowing aqueous fluid into a first conduit from a supply of aqueous fluid and flowing into the first conduit a spacing liquid supplied from a second conduit, the spacing liquid being immiscible with the aqueous fluid. The flowing of the aqueous fluid and the spacing liquid into the first conduit forms discrete volumes of the aqueous fluid, with consecutive discrete volumes of the aqueous fluid separated by the spacing liquid. The method may further comprise transferring the discrete volumes of the aqueous fluid and spacing liquid from the first conduit to a third conduit for processing.

The present invention involves a fill needle system for aseptically dispensing a pharmaceutical fluid in an aseptic chamber comprises a fill needle tubing in fluid communication with a pharmaceutical fluid source via flexible tubing and extending through a fill needle hub; a fill needle dispensing tip disposed at a dispensing end of the fill needle tubing; a fill needle sheath shaped and arranged to removably mate with and seal aseptically to the fill needle hub to form an aseptically sealed volume enclosing the dispensing tip; and a fluid pressure pulse induction system disposed and configured to compress the flexible tubing in order to dislodge droplets of pharmaceutical fluid retained on the dispensing tip after halting dispensing of the pharmaceutical fluid. An associated method of dispensing pharmaceutical fluid comprises operating the fluid pressure pulse induction system to dislodge the droplets. The system may comprise a controller for automatically controlling the dispensing and droplet dislodging.

A fluid sampler system includes a fluidic head including a gas aperture capable of communication with a pressurized gas source, a liquid conduit capable of communication with a sample reservoir, and a seal capable of forming a compression seal to the sample reservoir when gas pressure is applied to the sample reservoir through the gas aperture and capable of displacing a portion of the sample through the liquid conduit; and a positioning apparatus capable of positioning the fluidic head and the sample reservoir in communication with one another. A method for providing an aliquot of sample, includes forming a releasable compression seal to a reservoir including a liquid sample; pressurizing the reservoir with compressed gas; displacing an aliquot of the sample from the reservoir; and transferring the displaced aliquot of the sample to a location.

According to the present invention there is provided an assembly comprising, a needle unit comprising n hollow needles wherein n is greater than one, and wherein each hollow needle can receive a respective sample fluid; a flow cell unit comprising m flow cells wherein m is greater than one, each flow cell having an input and an output, and a test surface on which ligands can be provided located between the input, and output; a means for consecutively moving sample fluids, from each of said n hollow needles respectively, into all said m flow cells, so that said sample fluids flow consecutively through the same flow cells. There is further provided a corresponding method of screening a sample fluid for molecules which can bind to predefined ligands.

Method and devices are provided for assessing tissue samples from a plurality of tissue sites in a subject using molecular analysis. In certain aspects, devices of the embodiments allow for the collection of liquid tissue samples and delivery of the samples for mass spectrometry analysis.

A device and a method of using the device for determining hematocrit in a whole blood sample. The device includes a first portion having an introducer, at least one fluid channel, a fluid actuator, and an analysis sensor and conductivity sensor disposed within the fluid channel. The second portion includes at least one well containing at least one material. The first portion and second portion are movable with respect to each other. The introducer is configured to transfer at least a portion of the material from the well in portion two into the fluid channel of portion one. The method includes measuring the resistance over substantially the entire portion of a whole blood sample and calculating an average hematocrit level of the whole blood sample based on the measured resistance.

Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.

The present invention involves a fill needle system for aseptically dispensing a pharmaceutical fluid in an aseptic chamber comprises a fill needle tubing in fluid communication with a pharmaceutical fluid source via flexible tubing and extending through a fill needle hub; a fill needle dispensing tip disposed at a dispensing end of the fill needle tubing; a fill needle sheath shaped and arranged to removably mate with and seal aseptically to the fill needle hub to form an aseptically sealed volume enclosing the dispensing tip; and a fluid pressure pulse induction system disposed and configured to compress the flexible tubing in order to dislodge droplets of pharmaceutical fluid retained on the dispensing tip after halting dispensing of the pharmaceutical fluid. An associated method of dispensing pharmaceutical fluid comprises operating the fluid pressure pulse induction system to dislodge the droplets. The system may comprise a controller for automatically controlling the dispensing and droplet dislodging.

The disclosure is related to a liquid processing module, a liquid testing system and a testing method. The liquid processing module includes a base, a first valve and at least one negative pressure generator. The base has a first groove and at least one first opening. The first opening is connected to the first groove. The first valve is slidably located in the first groove. The negative pressure generator is configured to generate or remove negative pressure at the first opening in response to the first opening and the first groove being connected with each other.

Disclosed is a liquid sending method for sending liquid to a sample processing chip having a flow path formed therein, the method including: measuring a flow of a second liquid which is different from a first liquid which is introduced into the flow path in the sample processing chip; controlling the flow of the second liquid on the basis of the measured flow; and introducing the first liquid into the flow path in the sample processing chip by means of the second liquid of which flow is controlled.