The present invention relates to methods and systems for testing for the presence of a material such as one or more analyte types within a sample and more particularly, for improved single enzyme-linked immunosorbent assay (sELISA) testing as well as other variants of single-enzyme linked molecular analysis (SELMA). The invention involves flow systems for digital counting of analytes with at least one opening (inlet/outlet). A support with hydrophilic and hydrophobic patches preferably harbours capture probes immobilised on the hydrophilic features. Nano-to-attoliter droplets are formed on the hydrophilic features. A gas phase (called gas phase seal) is applied to prevent/reduce evaporation from the droplets.

Disclosed is a device for preparing a layer of biological cells on a deposition surface of a slide, the device including a body provided with a channel, the body including a first end forming an inlet tip intended to enter inside a container containing a biological liquid, closed by a cap and a second end forming a depositing tip intended to deposit a drop of the liquid on the deposition surface, the second end including a spreader with a blade arranged in a plane forming an angle of inclination with the plane of the deposition surface and elastic return interposed between the blade and the second end.

The present invention provides devices, systems, and methods, for performing biological and chemical assays.

A solution attachment device includes: an ejection tool having an ejection opening that ejects a solution in a direction of gravitational force; and a solution attachment control portion for controlling attachment of the solution ejected from the ejection opening to at least one attachment subject region of the solution formed on a substrate in a state in which the at least one attachment subject region is disposed below an end surface including the ejection opening of the ejection tool in the direction of gravitational force, and, in the state, a first projection region obtained by projecting the end surface to a surface perpendicular to the direction of gravitational force substantially overlaps a second projection region obtained by projecting the at least one attachment subject region to the surface.

A method for producing a plurality of measurement regions on a chip, and a chip having a plurality of measurement regions which is obtainable by the method.

An example of a flow cell includes a substrate, which includes nano-depressions defined in a surface of the substrate, and interstitial regions separating the nano-depressions. A hydrophobic material layer has a surface that is at least substantially co-planar with the interstitial regions and is positioned to define a hydrophobic barrier around respective sub-sets of the nano-depressions.

The present invention provides an apparatus for conducting biological assays which employs virtual wells in lieu of the physical wells of conventional array plates. Also provided are methods of processing a sample and/or culturing cells using the apparatus and systems described herein. In some embodiments, the apparatus includes a first structure having a sheet layer with a plurality of discrete through holes; and a second structure coupled to the first structure, the second structure including a base layer. At least a portion of a first surface of the sheet layer of the first structure is exposed from the second structure, and a second surface of the sheet layer, opposite to the first surface of the sheet layer, is embedded in the base layer of the second structure adjacent the first surface of the base layer.

This disclosure provides methods and apparatuses for sorting target particles. In various embodiments, the disclosure provides a cassette for sorting target particles, methods for sorting target particles, methods of loading a microchannel for maintaining sample material viability, methods of quantifying sample material, and an optical apparatus for laser scanning and particle sorting.

Devices, systems, methods, and kits are disclosed for amplifying small quantities of nucleic acid and identifying the nucleic acid sequences of the amplified nucleic acid products.

A scanning micro-fluid device for an exchange of species with a surface and with an intermediate immersion liquid is disclosed. The device comprises a first and a second micro-channel comprising a fluid. The first micro-channel comprises a first aperture and the second micro-channel comprises a second aperture. They have a distance to each other in an apex area in proximity of the surface of a substrate. The surface, the apex area is immersed with the intermediate immersion liquid. The device also comprises a first electrode reaching into the fluid on the first micro-channel and a second electrode reaching into the fluid on the second micro-channel, and an apex electrode. Different voltage levels are applicable to the first, the second and the apex electrode such that species are interacting at surface of the substrate.