An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.

An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.

The invention relates to a method for producing at least one pattern (2) on a carrier surface (7) of a carrier (3), wherein the method comprises the following steps: a. adding a first fluid (4) to the carrier surface (7) and b. adding a second fluid (5), wherein the second fluid (5) is immiscible with the first fluid (4) and at least partially covers the first fluid (4) and c. adding at least one object (6) above the carrier surface (7), d. generating the pattern (2) by a relative movement between the object (6) and the carrier (3) in which a force is exerted on the object (6) from a force generating means (28), wherein the force transmission from the force generating means (28) on the object (6) is contactless and the pattern (2) is generated by a portion of the second fluid (15) wetting the carrier surface (7).

A detection chip, a using method for the same, and a reaction system. The detection chip includes a first substrate, a micro-cavity defining layer, and a heating electrode. The micro-cavity defining layer is on the first substrate and defines a plurality of micro-reaction chambers. The heating electrode is on the first substrate and is closer to the first substrate than the micro-cavity defining layer, and is configured to heat a plurality of micro-reaction chambers. The orthographic projection of the plurality of micro-reaction chambers on the first substrate is within the orthographic projection of the heating electrode on the first substrate.

The present invention relates to a nanowell diagnostic kit for diagnosing a cardiovascular disease substance in blood, including: an electrode on which a plurality of nanowells are formed to accommodate a target substance; and a marker fixed to each of the plurality of nanowells of the electrode and responding to the target substance, wherein the target substance is identified through an electrochemical analysis method by applying a current to the electrode.

This disclosure provides methods and apparatuses for sorting target particles. In various embodiments, the disclosure provides a cassette for sorting target particles, methods for sorting target particles, methods of loading a microchannel for maintaining sample material viability, methods of quantifying sample material, and an optical apparatus for laser scanning and particle sorting.

The invention discloses a microfluidic device for the culture, selection and/or analysis of sample organisms such as nematodes, as well as for other biological entities such as for instance animal embryos. The device features reservoirs, culture chambers and smart filtering systems allowing for the selection of specific populations/specimens of sample organisms, thus permitting long-term cultures thereof as well as phenotypic/behavioural analyses. Systems and methods for using the microfluidic device are within the present disclosure as well.

Methods for performing multiple omics analysis in parallel are provided, the methods can include: dividing the mixture of cells or cell components into at least a first portion and a second portion; performing a first analysis on the first portion to acquire a first set of analytical data; performing a second analysis on the second portion to acquire a second set of analytical data. Methods for forming mixtures of analytes into first and second portions are also provided. The methods can include aligning the first and second plates to engage the first exposed surface with the second exposed surface, wherein the engaging is sufficient to convey at least some of the first analytes into the second solution to form a second mixture of the first analytes.

Methods for forming arrays of droplets, and associated arrays of droplets, are generally provided.

There is provided a sample support capable of easily placing a sample into position. The sample support is used such that a sample floating on the surface of water is scooped and held. The sample support has: a first region on which the sample is to be placed; and a second region of higher wettability than the first region.