A diagnostic method for a detection of a nuclear deformation based a cellular differentiation includes the following steps: preparing nano/micropatterned surfaces; performing a cell seeding on the nano/micropatterned surfaces; imaging; analyzing nuclear deformations of a single cell and a cell population with an algorithm method.

The present invention relates to a patterned substrate comprising first regions having first dendrimer structures and second regions having second dendrimer structures on a surface of the substrate, as well as a to method for manufacturing a patterned substrate and the use of a patterned substrate for the chemical synthesis of a chemical synthesis product, as a characterizing platform and/or as a platform for cell treatment and/or cell cultivation.

A liquid evaluation system can include a cartridge including a channel configured to pull a liquid into the channel by capillary action. The cartridge can include an upper plate and a lower plate located in close proximity to the upper plate. The lower plate includes a deposition region for dispensing liquid. The extent of the deposition region in one or more directions can be defined by a repellant barrier, such as a boundary with a set of areas configured to be repellant to the liquid. An internal facing surface of each plate can include a corresponding region forming the channel. Each of the regions can be configured to have an affinity for the liquid. The close proximity of the plates and the regions having an affinity for the liquid cause the liquid to be pulled into the channel by capillary action.

An open fluid droplet ejection cartridge containing one or more fluids to be dispensed by digital fluid dispense system and a method for digitally dispensing fluids. The open fluid droplet ejection cartridge includes a cover having one or more openings therein, fluid funnels pending from each of the one or more openings in the cover, a chamber separator attached to the fluid funnels for directing fluid to one or more fluid chambers, a fluid overflow chamber for fluid overflow from the one or more fluid chambers, fluid vias associated with each of the one or more fluid chambers, and a plurality of fluid ejection devices adjacent to the fluid vias on a single semiconductor substrate in fluid flow communication with the fluid vias.

This disclosure provides methods and apparatuses for sorting target particles. In various embodiments, the disclosure provides a cassette for sorting target particles, methods for sorting target particles, methods of loading a microchannel for maintaining sample material viability, methods of quantifying sample material, and an optical apparatus for laser scanning and particle sorting.

The present disclosure relates to digital microfluidic systems having an electrode bus controlled by a single actuation input, and methods for droplet manipulation using the electrode bus. Particularly, aspects are directed to a digital microfluidic system including a first group of droplet actuation electrodes formed in a substrate, a first wiring bus formed in the substrate and connected to each electrode in the first group of droplet actuation electrodes, and a first single point of actuation connected to the first wiring bus; and a second group of droplet actuation electrodes formed in the substrate, a second wiring bus formed in the substrate and connected to each electrode in the second group of droplet actuation electrodes, and a second single point of actuation connected to the second wiring bus.

Example implementations include a sensor device with a superhydrophobic sensor panel having an abraded first planar surface and a second planar surface opposite to the first planar surface, and a metallic heating element adjacent to the second planar surface of the superhydrophobic sensor panel. Example implementations also include a method of detecting a concentration of heavy metal ions in a solution, by separating a target solute of a target microdroplet from the target micropdroplet, identifying a distribution area of at least one heavy metal ion in an image of the target solute, generating a heavy metal ion concentration quantity based on the distribution area, generating a composite image including an indication of the distribution area, and presenting an indication of at least one of the heavy metal ion concentration quantity and the composite heavy metal ion image. Example implementations also include a method of manufacturing a heavy metal ion sensor device, by abrading a first planar surface of a superhydrophobic sensor panel, depositing a metallic layer on a nonconductive substrate, and contacting the metallic layer to a second planar surface of the sensor panel opposite to the first planar surface.

The present disclosure provides devices, methods, and systems for performing cellular, biological, or chemical assays where a sample can have a plurality of analytes.

An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.

A method for washing particles includes obtaining an array plate that includes an array of hydrophilic areas surrounded by one or more hydrophobic areas. A respective solution containing a sample is located on a respective hydrophilic area of the array of hydrophilic areas. The respective hydrophilic area includes one or more indentations from a respective surrounding hydrophobic area of the one or more hydrophobic areas. The respective hydrophilic area includes a first indented surface that is offset from a reference surface defined by the respective surrounding hydrophobic area. The method also includes placing an aspirator nozzle above the respective hydrophilic area at a predefined distance from the first indented surface, and aspirating the solution with the aspirator nozzle while the aspirator nozzle is located at the predefined distance from the first indented surface.