One aspect of the present invention is to provide the device and methods for performing an assay that uses the multiplexing of sample thicknesses on the same plate. The sample thickness multiplexing can offer many information that are unavailable in using a single sample thickness.

A device comprising a base and a support structure is disclosed. The base includes a vial receptacle for holding a vial. The support structure is coupled to an upper portion of the base and is configured to align a pipette comprising a pipette tip vertically over the vial receptacle. The support structure is configured to support a pipette such that an end of a pipette tip is positioned one or more predetermined depths within a vial in the vial receptacle.

A method for withdrawing a solution comprising a plurality of particles from a vial is disclosed. The method includes agitating the vial at a first predetermined mixing speed to suspend the plurality of particles in the solution. The method also includes withdrawing, during the agitating, a first volume of the solution from the vial with a pipette, such that an end of a pipette tip is positioned at a first predetermined depth within the vial.

A method for preparing an aliquot of solution comprising a plurality of particles is disclosed. The method includes agitating the solution with a baffle at a predetermined mixing speed to suspend the plurality of particles in the solution, wherein the baffle positioned at least partially within a vessel containing the solution, and wherein the vessel is engaged with a vessel receptacle configured to hold the vessel. The method also includes withdrawing, during the agitating, the aliquot of solution from the vessel with a pipette, such that an end of a pipette tip is positioned within the vessel.

A variable spacing PCR amp tube rack can include a plurality of rotors and a plurality of carriages engaged with the rotors so that rotation of the rotors causes translation of the carriages with respect to one another. The rack can include a blade assembly that allows an operator to separate sets of coupled PCR amp tubes from one another. The rack can be designed to accommodate 0.1 ml PCR amp tubes and adjust their spacing from 4.5 mm to 9.0 mm.

A pipetting head comprises a carrier, at least one attachment held on the carrier and configured to secure at least one pipette tip, and two O-rings retained on the at least one attachment and configured to contact the sealing seat of the at least one pipette tip. The inner diameter of the sealing seat of the at least one pipette tip is smaller than an outer diameter of a respective O-ring where the sealing seat contacts a respective O-ring. The respective O-ring is deformed while the pipette tip is slid onto the at least one attachment to securely clamp the at least one pipette tip. The sealing seat of the at least one pipette tip is dimensioned such that the at least one attachment exerts a force on the pipette tip to deform the at least one pipette tip when it is securely clamped on the at least one attachment.

Multi-well collapsible basket arrays and methods for their use with high throughput culture and histology analysis of spheroids and organoids. Such a collapsible basket array includes a collapsible cellular array structure having multiple cells and connectors that interconnect adjacent pairs of the cells to cause the collapsible cellular array structure to collapse from an expanded configuration to a collapsed configuration in which the connectors are partially wrapped around perimeters or circumferences of the cells, whereby the collapsible cellular array structure is expandable to acquire an expanded configuration capable of individually aligning the cells thereof with wells of a well plate. The collapsible basket array further includes inserts individually mountable to the cells of the collapsible cellular array structure, with each insert including a permeable basket with pores sized to retain spheroids or organoids within the basket.

A test device includes a housing and a lid. The housing encloses an internal space, has a hole supporting a container accommodating liquid, and includes a perforation/incision part. The lid covers a hole-formed part of the housing. The housing and/or the lid includes a guide that guides the housing and the lid, so that the lid can migrate from the first position to the second position while covering the hole-formed part of the housing. In the first position, the lid covers the hole-formed part of the housing and the container and the container is not incised. During the migration of the lid from the first position to the second position, the container is pushed toward the perforation/incision part by the lid, and the container is incised to leak liquid into the internal space.

A stepped biological chip and a gene sequencing device for testing same. The stepped biological chip comprises substrates and small small small small small small fluorescent balls carrying biological information arranged on the top of the substrates, and the heights of the center point of adjacent small small small small small small fluorescent balls from a substrate bottom edge A are incremented or decremented by equal value. The gene sequencing device comprises a chip placing platform, the stepped biological chip, and a microscope objective lens placed above the chip placing platform and an illumination light source irradiated on the stepped biological chip at a certain angle of incidence.

A separation and collection device for cells and biomolecules includes an annular tube and several magnetic beads configured to identify and bind with different cells or molecules in the biological sample. The annular tube defines a through hole for the biological sample to exit. Sizes of different groups of magnetic beads are different. An aperture size of a through hole is changeable such that the plurality of groups of magnetic beads can flow through the through hole in a certain order. A testing system including the separation and collection device is further disclosed.