A microfluidic chip configuration wherein injection occurs in an upwards vertical direction, and fluid vessels are located below the chip in order to minimize particle settling before and at the analysis portion of the chip's channels. The input and fluid flow up through the bottom of the chip, in one aspect using a manifold, which avoids orthogonal re-orientation of fluid dynamics. The contents of the vial are located below the chip and pumped upwards and vertically directly into the first channel of the chip. A long channel extends from the bottom of the chip to near the top of the chip. Then the channel takes a short horizontal turn that nearly negates any influence of cell settling due to gravity and zero flow velocity at the walls. The fluid is pumped up to a horizontal analysis portion that is the highest channel/fluidic point in the chip and thus close to the top of the chip, which results in clearer imaging. A laser may also suspend cells or particles in this channel during analysis which prevents them from settling.

A microfluidic device includes a lower casing and an upper casing covering the lower casing. The lower casing includes a lower base wall having a top surface and a plurality of spaced-apart columns that protrude upwards from the top surface. The upper casing includes an upper base wall. A first gap between the upper base wall and a column top surface of each of the columns is large enough to permit passage of large biological particles of a liquid sample, and a second gap between any two adjacent ones of the columns is not large enough to permit passage of the large biological particles and is large enough to permit passage of small biological particles of the liquid sample.

A system for assembling fields from a source substrate onto a second substrate. The source substrate includes fields. The system further includes a transfer chuck that is used to pick at least four of the fields from the source substrate in parallel to be transferred to the second substrate, where the relative positions of the at least four of the fields is predetermined.

A system for processing biological particles including bioprocessing microfluidic devices, reservoirs, buffer tanks and two fluidic connection systems. A first fluidic connection system includes valves and connecting elements between valves, so that each reservoir or port configured to connect a reservoir may be in fluidic connection with each buffer tank, and a second fluidic connection system includes valves and connecting elements between valves, so that each bioprocessing microfluidic device may be in fluidic connection with each buffer tank.

This relates to acoustic microfluidic systems that can generate emulsions/droplets or encapsulate particles of interest (including mammalian cells, bacteria cells, or other cells) into droplets upon detection of the particles of interest flowing in a stream of particles. The systems operate on the detect/decide/deflect principle wherein the deflection step, in a single operation, not only deflects particles of interest from a stream of particles but also encapsulates the particles of interest in an emulsion droplet. The microfluidic systems have an abrupt transition in the channel geometry from a shorter channel to a taller channel (i.e., in the shape of a ‘step’) to break the stream of the dispersed phase into a droplet upon acoustic actuation. When there is no acoustic wave present, no droplets/emulsions are generated and the stream of particles proceeds uninterrupted. The rapid actuation and post-actuation recovery employed by the microfluidic systems taught herein ensure that the vast majority of selected particles are properly deflected, that few or no empty droplets are produced, and that total throughput remains high.

A device, liquid handling cartridge and related method for detecting the presence or absence of a chemical or biological target within a sample. The method includes the steps of: providing an electrochemical cell with a first electrode module and a second electrode; providing an electronic component between the first electrode module and the second electrode; introducing the sample into the electrochemical cell; measuring the potential difference between the first electrode module and second electrode; and confirming the presence of the chemical or biological target if the measured potential difference exceeds a predetermined threshold value.

In one example in accordance with the present disclosure, a fluid ejection die is described. The fluid ejection die includes a fluid feed slot to deliver fluid from a reservoir to an array of ejection chambers fluid connected to the fluid feed slot. Each ejection chamber includes at least one fluid actuator and an opening through which fluid is to be ejected. The fluid ejection die also includes a number of antechambers. An antechamber includes sidewalls that curve inward.

Disclosed are a microfluidic system and method for isolating target cells or vesicles in a fluid. The system of the present invention comprises a fluid passageway having an inlet and an outlet; one or more ultra-high frequency acoustic resonator capable of generating bulk acoustic waves in the fluid passageway at a frequency of about 0.5-50 GHz; a power regulator which adjusts the power of the bulk acoustic waves generated by the ultra-high frequency resonator; and a flow rate regulating device that regulates the velocity of the solution flowing through the bulk acoustic wave region. Adjusting the power of the generated bulk acoustic waves by means of the power regulator and/or adjusting the velocity of the solution flowing through the bulk acoustic wave region by means of the flow rate regulating device allow cells or vesicles to stay in a bulk acoustic wave-affected region. The system and method of the present invention can capture and release cells or vesicles in a solution, and further process and analyze the obtained cells or vesicles.

The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.