The present invention relates to microfluidic fluidic devices, methods and systems as microfluidic kidney on-chips, e.g. human Proximal Tubule-Chip.

An apparatus for sorting macromolecules includes a first chip including a channel formed in a first side of the first chip and having at least one monolithic sorting structure for sorting macromolecules from the sample fluid. A first set of vias formed in the first chip has openings in a second side of the first chip, the sample fluid being provided to the sorting structure through the first set of vias. A second set of vias formed in the first chip has openings in the second side for receiving macromolecules in the sample fluid greater than or equal to a prescribed dimension sorted by the sorting structure. A third set of vias formed in the first chip has openings in the second side for receiving macromolecules in the sample fluid less than the prescribed dimension. The apparatus includes first and second seals covering the first and second sides, respectively.

The present invention relates to a biosensor, including: a blood cell separation membrane which separates blood cells from blood and allows plasma components to pass through; a microfluid channel through which the plasma components that have passed through the blood cell separation membrane flow; a lower substrate which allows the plasma components that have passed through the blood cell separation membrane to flow along the microfluid channel; and a pillar which connects the blood cell separation membrane and the lower substrate, in which an electrode is disposed in the pillar, and the pillar pushes and lifts the blood cell separation membrane by a predetermined distance. The biosensor of the present invention allows plasma, which is difficult to pass through the blood cell separation membrane due to surface tension, to easily pass through.

A method herein to isolate exosomes includes providing a microfluidic device having a spiral-shaped channel in fluid communication with two inlet ports and at least two outlet ports. One of the two inlet ports is proximal to an inner wall of the spiral-shaped channel and the other is proximal to an outer wall thereof. At least one of the outlet ports is in fluid communication with a container for storing isolated exosomes. A blood sample and sheath fluid are introduced into the inlet ports proximal to the outer and inner walls, respectively, to form a diluted sample in the spiral-shaped channel and driven through for exosomes recovery in the container. The spiral-shaped channel in fluid communication with a first outlet port includes a first outlet channel connecting the spiral-shaped channel to the first outlet port and is longer than other outlet channels respectively connecting the spiral-shaped channel to the other outlet ports. A method of identifying diabetes mellitus is also disclosed herein.

Provided is a new method for more efficiently generating emulsion particles each containing one fine particle.

The present technology provides a method of collecting fine particles, in which in a fine particle sorting mechanism having a channel structure including a main channel through which the fine particles flow, a collection channel into which particles to be collected are collected from among the fine particles, a connection channel that connects the main channel and the collection channel, and a liquid supply channel connected to the connection channel so as to supply a liquid, the method includes: a flow step of causing a first liquid containing the fine particles to flow through the main channel; a determination step of determining whether or not the fine particles flowing through the main channel are the particles to be collected; and a collection step of collecting the particles to be collected into the collection channel, and, in the collection step, the particles to be collected are collected into a second liquid that is immiscible with the first liquid in the collection channel while being contained in the first liquid.

A microfluidic device includes a bottom electrode, a dielectric layer on the bottom electrode, one or more top electrodes on a region of the dielectric layer, Each of the one or more top electrodes has a sidewall that forms a sidewall angle with an outer surface of the dielectric layer that is less than 180 degrees. The sidewall of each of the one or more top electrodes and a portion of the outer surface of the dielectric layer adjacent to the sidewall define a microchannel region for transporting an open microchannel of a fluid. Such microfluidic devices may enable transport of small microchannels using low voltages.

The present invention relates to the field of microfluidics and in particular to devices and methods for sorting objects in microfluidic channels. These devices and methods allow for fast and robust sorting in two-way and multi-way setups. They also enable sorting over extended periods of time.

Embodiments of the current disclosure are directed to systems, methods and apparatus for evaluating single cell secretion profiles. In some embodiments, the apparatus may be configured to analyze substances expressed by a biological cell and may include a first compressible substrate, and a second substrate configured for removable sealing attachment with the first substrate. In some embodiments, upon attachment of the second substrate with the first substrate, an assembly is formed such that the open side of the plurality of chambers are covered by the second substrate, and a portion of each of the plurality of capture areas are exposed in each of the chambers.

The objective of the present invention is to provide a particle detection device and a particle detection method that can individually and continuously detect a wide range of particles. The objective is achieved by a particle detection device including: a particle separation channel through which particles are separated according to particle sizes in a perpendicular direction to the flow of fluid; and two or more particle recovery channels that are connected to and branched from the particle separation channel, in which each of the particle recovery channels includes a particle detection unit that includes an aperture and an electric detector.

To provide a technology of optimizing a suction condition of a microparticle. The present technology provides an optimizing method of a suction condition of a microparticle including: a particle number counting step of detecting a time point when a microparticle passes through a predetermined position on a main flow path through which liquid containing the microparticle flows, sucking the microparticle from the main flow path to a microparticle suction flow path by the microparticle suction flow path with a predetermined suction force, and counting the number of microparticles sucked into the microparticle suction flow path; and a step of determining an elapsed time from passage through the predetermined position with which the suction by the microparticle suction flow path should be performed on the basis of a time from the time point when the microparticle passes through the predetermined position on the main flow path until the suction is performed and the number of counted microparticles.