Cell separation systems, and methods for separating cells from microcarriers, and harvesting the separated cells, are provided, wherein the system comprises a cell separation device, a cell settling device, and a cell screening device.

A circulating tumor cell capture device includes a chip system and a pump. The chip system includes a confluence chip and a lower chip set. The lower chip set is disposed at a lower surface of the confluence chip, and includes a channel chip, a split chip and a porous membrane. The channel chip is disposed at the lower surface of the confluence chip. The split chip is detachably stacked below the channel chip. The porous membrane is embedded in the channel chip. The specimen passes through the porous membrane and flow between the channel chip and the split chip to make the circulating tumor cell be captured by the porous membrane.

A device for blood (1) is provided with a column (50) and a micro flow path (20) located downstream of the column (50). The column (50) includes a porous material as a solid phase, and blood that has contacted with the porous material flows through the micro flow path (20). In the device for blood (1), the column (50) and the micro flow path (20) are provided as separated bodies. The column (50) has a connecting part (55), the micro flow path (20) has an inlet (21a), the connecting part (55) and the inlet (21a) are connected to each other to integrate the column (50) with the micro flow path (20), and blood (BL) is allowed to pass from the column (50).

The present invention provides compositions, systems, kits, and methods for analyzing the interaction of T-cells and neoantigen presenting cells (and other cells) via discrete entity (e.g., droplet) microfluids. In certain embodiments, a microfluidic device is used to merge a discrete entity containing a T-cell, and a discrete entity containing a neoantigen presenting cell, at a merger region via a trapping element in order to generate a combined discrete entity. In particular embodiments, at least one thousand of such combined discrete entities are formed in about one second. In some embodiments, whether the receptor on the T-cell sufficiently binds the neoantigen to activate the T-Cell is detected (e.g., via detection of cytokine or granzyme B release). In certain embodiments, provided herein are methods for identifying polyfunctional T-cells or NK-cells, as well as methods of screening for such cells that would be cytotoxic if injected into a subject.

A method for separating cells in a biofluid includes pretreating the biofluid by introducing a predetermined amount of a cocktail of antibodies, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the pretreated biofluid within the microfluidic separation channel. A system for microfluidic cell separation, capable of separating target cells from non-target cells in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of an additive including the cocktail of antibodies, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic cell separation is also disclosed. A method of facilitating separation of cells is also disclosed.

The present application provides a method and a system for integrating morphological characteristics and gene expression of individual cells. The method comprises the following steps: providing a microfluidic device, which comprises a microwell array and an interdigital electrode, and each microwell comprises a plurality of capture oligonucleotides; injecting cells into the microwells, capturing a single cell and recording morphological characteristics of the cell; lysing the cell so that the mRNA released by the cell is captured by the capture oligonucleotide; reverse transcribing the captured mRNA to obtain cDNA; performing a PCR amplification reaction on the cDNA to obtain a cDNA library and sequencing the cDNA library; reading the cell barcode sequence and the unique molecular identifier sequence according to sequencing results, and the morphological characteristics and gene expression of the cell in the microwell are integrated together.

The present disclosure is related to a method of producing a microfluidic sorting apparatus. The method includes providing an injection-molded substrate comprising a network of channels; bonding an insulating film to an upper surface of the substrate to cover the network of channels; and depositing a conductive film on the insulating film. The substrate can be separated from the conductive film.

A passive, hydrodynamic technique implemented using a microfluidic device to perform co-encapsulation of samples in droplets and sorting of said droplets is described herein. The hydrodynamic technique utilizes laminar flows and high shear liquid-liquid interfaces at a microfluidic junction to encapsulate samples in the droplets. A sorting mechanism is implemented to separate sample droplets from empty droplets. This technique can achieve a one-one-one encapsulation efficiency of about 80% and can significantly improve the droplet sequencing and related applications in single cell genomics and proteomics.

An electronically-controlled digital ferrofluidic device is disclosed which employs a network of individually addressable coils in conjunction with one or more movable permanent magnets, where each moveable permanent magnet delivers the designated fluid manipulation-based tasks. The underlying mechanism facilitating fluidic operations is realized by addressable electromagnetic actuation of miniaturized mobile magnets that exert localized magnetic body forces on droplets filled with magnetic nanoparticles. The reconfigurable, contactless, and non-interfering magnetic-field operation properties of the underlying actuation mechanism allow for the integration of passive and active components to implement advanced and diverse operations with high efficiency (e.g., droplet sorting, dispensing, generation, merging, mixing, filtering, and analysis).

To provide a particle sorting kit provided with a filter that functions even at a small flow rate with a small loss amount of particles.

Provided is a particle sorting kit provided with a sample accommodation unit configured to accommodate a sample liquid containing particles, a microchip provided with a sample flow path through which the sample liquid flows and a sorting flow path in which target particles are sorted from the sample liquid, and a filter unit provided with a filter and a tapered portion that decreases a flow path diameter in a flow direction downstream of the filter.