Among others, the present invention provides apparatus for detecting a disease, comprising a system delivery biological subject and a probing and detecting device, wherein the probing and detecting device includes a first micro-device and a first substrate supporting the first micro-device, the first micro-device contacts a biologic material to be detected and is capable of measuring at the microscopic level an electric, magnetic, electromagnetic, thermal, optical, acoustical, biological, chemical, physical, or mechanical property of the biologic material.

A flow cell article including: a chamber; and at least one surface of the chamber comprising: a solid substrate having a reactive surface comprising: a coupling agent covalently attached to the solid substrate; a polymer of the formula (I) as defined herein, covalently attached to the coupling agent;

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a nucleic acid probe covalently attached to the polymer. Also disclosed is a method of making the article and a method of using the article.

The invention concerns a method for measuring a force on at least one particle in a fluid wherein an inhomogeneous field of hydrodynamic flows is generated in a fluid by specific dynamic localized heating events, the particle is spatially manipulated by the hydrodynamic flows, a spatial configuration of the particle(s) within the fluid is captured and at least one force acting on the particle(s) is determined by evaluating the captured spatial configuration of the particle(s). The invention concerns furthermore an apparatus for measuring a force on at least one particle in a fluid, a computer program product, and a computer-readable storage medium.

A method is disclosed for measuring a temperature of a fluid in a holding space of an acoustic-based particle manipulation device. The method comprises performing a calibration. The calibration comprises measuring, using a reference temperature sensor, the temperature of the fluid to be a reference temperature value. The calibration also comprises determining a reference resonance frequency of the acoustic-based particle manipulation device having the fluid in its holding space at the reference temperature value. The method further comprises measuring the temperature of the fluid comprising determining a resonance frequency of the acoustic-based particle manipulation device and determining, based on the reference resonance frequency determined during the calibration and based on the determined resonance frequency, the temperature of the fluid to be a temperature value.

An object is to stably, continuously and easily detect reactions of filamentary biomolecules or polymers bridging between a pair of electrodes against reagents without employing any marker or labeling substances. A molecule detecting system for detecting a reaction of a molecule captured between electrodes to a reagent in a liquid 37, the system comprising: a detection device including a couple of electrodes and being capable of measuring a resonance frequency and an amplitude of the electrodes; a microfluidic device 30 including a microfluidic channel 36 which includes a channel opening 36c on its one side, wherein an air-liquid interface the electrodes can pass through is formed in the channel opening 36c; a pressure controlled microfluidic pump 33 connected to an outlet 36b of the microfluidic channel 36; a movable holding device movably holding the detection device or the microfluidic device 30; and a controller controlling the detection device, the pressure controlled microfluidic pump 33, and the movable holding device.

Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ?1-nm aperture tortuous nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.

Among others, the present invention provides apparatus for detecting a disease, comprising a system delivery biological subject and a probing and detecting device, wherein the probing and detecting device includes a first micro-device and a first substrate supporting the first micro-device, the first micro-device contacts a biologic material to be detected and is capable of measuring at the microscopic level an electric, magnetic, electromagnetic, thermal, optical, acoustical, biological, chemical, physical, or mechanical property of the biologic material.

Provided are integrated analysis devices having features of macroscale and nanoscale dimensions, and devices that have reduced background signals and that reduce quenching of fluorophores disposed within the devices. Related methods of manufacturing these devices and of using these devices are also provided.

A system and method for determining a trajectory parameter of particles, comprising receiving a plurality of particles at a microfluidic channel, applying a force to each particle of the microfluidic channel, acquiring a dataset of each particle, measuring a trajectory of the particle, and determining a trajectory parameter of the particles.