The present application provides a method and a system for integrating morphological characteristics and gene expression of individual cells. The method comprises the following steps: providing a microfluidic device, which comprises a microwell array and an interdigital electrode, and each microwell comprises a plurality of capture oligonucleotides; injecting cells into the microwells, capturing a single cell and recording morphological characteristics of the cell; lysing the cell so that the mRNA released by the cell is captured by the capture oligonucleotide; reverse transcribing the captured mRNA to obtain cDNA; performing a PCR amplification reaction on the cDNA to obtain a cDNA library and sequencing the cDNA library; reading the cell barcode sequence and the unique molecular identifier sequence according to sequencing results, and the morphological characteristics and gene expression of the cell in the microwell are integrated together.

The present invention is directed to systems and devices that allow for separation of cells based on size and electric properties and for high-throughput cell sorting. The system may comprise a microfluidic platform having a main microfluidic channel and cavity acoustic transducers (CATs). The microfluidic platform may be coupled to an external acoustic source. The system may further comprise a fluid disposed through the main microfluidic channel comprising cells having different sizes and electric properties. The fluid may intersect the CATs to form one or more interfaces. The system may further comprise electrodes underneath the microfluidic platform. The CATs may oscillate the interfaces to produce one or more microstreaming vortices, such that each microstreaming vortex is capable of selectively trapping cells based on size. The set of electrodes may apply an AC to cause the cells to move relative to the set of electrodes based on electric properties.

A method for separating and enriching sperm from a tissue sample comprises: obtaining a microfluidic separating system having an inlet end and an outlet end, and a membrane filter (e.g., hollow fiber membrane filter) fluidly connected to the outlet end; separating the tissue sample via the microfluidic separating system into a debris fluid volume and a sperm fluid volume; and enriching the sperm fluid volume by removing excess media via the membrane filter. A two-stage tissue sample separation system comprising: a microchannel structure defining a separation fluid channel to form a separation stage; an inlet end of the microchannel structure; an outlet end of the microchannel structure; and a membrane filter fluidly connected to the outlet end for removal of at least a portion of excess media in the tissue sample.

A passive, hydrodynamic technique implemented using a microfluidic device to perform co-encapsulation of samples in droplets and sorting of said droplets is described herein. The hydrodynamic technique utilizes laminar flows and high shear liquid-liquid interfaces at a microfluidic junction to encapsulate samples in the droplets. A sorting mechanism is implemented to separate sample droplets from empty droplets. This technique can achieve a one-one-one encapsulation efficiency of about 80% and can significantly improve the droplet sequencing and related applications in single cell genomics and proteomics.

Disclosed are methods, systems, and articles of manufacture for performing a process on biological samples. An analysis of biological samples in multiple regions of interest in a microfluidic device and a timeline correlated with the analysis may be identified. One or more region-of-interest types for the multiple regions of interest may be determined; and multiple characteristics may be determined for the biological samples based at least in part upon the one or more region-of-interest types. Associated data that respectively correspond to the multiple regions of interest in a user interface for at least a portion of the biological samples in the user interface based at least in part upon the multiple identifiers and the timeline. A count of the biological samples in a region of interest may be determined based at least in part upon a class or type of data using a convolutional neural network (CNN).

A device for imaging sensitive biological samples is provided. The device can include a plastic frame and a glass coverslip, each can be comprised of a biologically compatible material. The device can then be configured such that a biological sample placed therein can only be in contact with biologically compatible materials and, when imaged, provide optimal imaging characteristics by allowing imaging through the glass coverslip.

Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.

A fluidic device for capturing or detecting a sample substance contained in a solution includes at least two continuous circulation flow channels selected from the group consisting of: a first type continuous circulation flow channel which is formed of a first circulation flow channel and a second circulation flow channel and which is configured to circulate the solution in the first circulation flow channel and then circulate the solution in the second circulation flow channel; and a second type continuous circulation flow channel which is formed of a third circulation flow channel and a fourth circulation flow channel and which is configured to circulate the solution in the third circulation flow channel and then circulate and mix the solution in both of the third and fourth circulation flow channels, wherein any one of the circulation flow channels has a capturing section which captures the sample substance, and/or a detecting section which detects the sample substance.

A solid reagent containment unit is formed by a support; a frame body fixed to the support and delimiting internally, together with the support, an analysis volume; a reagent-adhesion structure within the analysis volume; and at least one reagent cavity, which extends within the reagent-adhesion structure. The reagent-adhesion structure is of an adhesion material embossable at temperatures lower by 6-8° C. than its own melting point and has a melting point such as not to interfere with the analysis. The reagent cavity forms a retention wall, laterally surrounding the reagent cavity, and houses dried reagents. The adhesion material is chosen among wax, such as paraffin, a polymer, such as polycaprolactone, a solid fat, such as cocoa butter, and a gel, such as hydrogel or organogel.

A liquid handling device includes a common channel, a plurality of wells, a magnetic beads chamber and a plurality of valves. The plurality of valves are rotary membrane valves disposed on the circumference of a first circle. The magnetic beads chamber is disposed on a circumference of the second circle concentric with the first circle.