The present invention is related to a portable apparatus for performing uni-directional convective qPCR or qRT-PCR in a mixing reagent containing a target nucleic acid and a fluorescence dye including denaturation, annealing and extension processes. The apparatus includes at least a temperature controlling unit which comprises at least one heat source and one temperature sensor, a circulation-enabling container, a light source, a photo-detector, a filter, a set of optical elements, and a processor.

The present invention provides a device and method for testing a material for the presence of DNA. The system includes a centrifuge, a microchip performing cell lysis and an enclosure that contains an isothermal ballast material and chromogenic agent that melts at a specific temperature and displays a color change, respectively.

The disclosure describes apparatus and methods for multiplexed amplification and detection of nucleic acid targets in a sample. Embodiments of the present disclosure include a mechanical system configured to provide loading, vertical positioning and clamping of a chip; a thermal control system configured to maintain distinct temperatures of the chip, and an optical fluorescence imaging system.

Provided herein, in some embodiments, are rapid diagnostic tests to detect one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens). In some embodiments, the pathogens are viral, bacterial, fungal, parasitic, or protozoan pathogens, such as SARS-CoV-2 or an influenza virus. In one embodiment, a diagnostic system is provided comprising a control device configured to control one or more parameters and/or actions of a diagnostic test, and a test kit component comprising a physical encoding of control information for the control device. In one embodiment, the control device is configured to receive the control information of the physical encoding and perform one or more actions based at least in part on the control information. In one embodiment, the control device can control one or more temperatures at which a biological sample is to be processed as part of the diagnostic test.

Disclosed is a reaction method that includes a reaction process using a pipette tip to allow substances to cause a reaction, the method including: providing a heater for heating the pipette tip in accordance with a preset temperature; attaching the pipette tip to a pipette nozzle and heating the pipette tip by a first preset temperature; when a period of heating by the first preset temperature exceeds a preset period, switching an output of the heater from the first preset temperature to a lower second preset temperature; detecting a distal end position of the pipette tip at or after the switching; and executing the reaction process while controlling the distal end position of the pipette tip with reference to the detected distal end position, in which the temperature of the pipette tip is kept by the second preset temperature at least until operation of the pipette tip.

The object of the invention is a device for conducting amplification reaction of biological samples with a system for independent control of the temperature of test tubes in a heating assembly comprising a multipart heating slot located in the cooling system housing, characterised in that the heating assembly comprises at least one heating slot (100) comprising a metal heating sleeve (101) wound around with a bifilar winding wire made of enamelled winding wire (102), which is covered with a composite polymer layer (103), wherein a temperature sensor (104) is located on the surface of the winding wire, and at least one heating slot is mounted on the PCB control board (105) located on the cooling system housing (112),

In one example in accordance with the present disclosure, a cell sorting device is described. The cell sorting device includes a microfluidic channel to serially transport individual cells from a volume of cells along a flow path. A sensor disposed in the microfluidic channel distinguishes between a cell to be analyzed and waste fluid. The cell sorting device includes at least two fluid transport devices disposed within the microfluidic channel. The at least two fluid transport devices include a cell ejector to, responsive to detection of a cell to be analyzed, eject the cell to be analyzed from the cell sorting device and a waste transport device to direct the waste fluid to a waste reservoir.

Provided is a sensing apparatus comprising a chip for integrated amplification and sequencing of a template polynucleotide in a sample. The apparatus comprises a chip with at least one ISFET in a well or chamber, amplification means for amplifying the template polynucleotide on a surface of said chip and comprising at least one heating means suitable for conducting amplification of the template polynucleotide at temperatures elevated with respect to room temperature, and sequencing means for sequencing the amplified template polynucleotide in said well or chamber. Methods of use are also provided.

The invention relates to the rapid enrichment of nucleic acid molecules of interest from complex mixtures of nucleic acids for the purpose of sequencing genes and variants, e.g. for clinical uses as well as other applications. A hybridisation column comprising an inner channel, wherein a portion of said channel is filled completely with a porous solid support comprising (a) a plurality of interconnected, micron-sized voids that permit a fluid to flow between them and the remainder of the channel, and (b) a plurality of hybridisation probes, which are bound to the surfaces of the solid support forming the voids is disclosed.

An object of the invention is to provide a method by which failure or performance deterioration of a temperature adjustment unit can be detected while specifying a cause part in a state where a nucleic acid amplification process is being performed. In order to achieve said purpose, an initial value of the level of control signals input to a temperature adjustment element during temperature maintenance, temperature rise, and temperature fall is set in advance and an error determination threshold is set on the basis of this value. Errors (malfunctions, performance deterioration) in the temperature adjustment unit are diagnosed and components causing said errors are identified, by comparing current control signal levels and threshold values monitored in a state in which the nucleic acid amplification process is being undertaken.