A device for base calling is provided. The device includes a receptacle configured to hold a biosensor having a sample surface holding a plurality of clusters during a sequence of sampling events, an array of sensors sensing information from clusters disposed in corresponding pixel areas of the sample surface during the sampling events and generate sequences of pixel signals and a communication port configured to output the sequences of pixel signals. The device also includes a signal processor coupled to the communication port and configured to receive and process at least one pixel signal in the sequences of pixel signals that mixes light gathered from at least two clusters in a corresponding pixel area, and to base call each of the at least two clusters using the at least one pixel signal.

A method to monitor and control the temperature of a sample holder of a laboratory instrument during execution of a temperature profile on the sample holder is presented. The laboratory instrument comprises a sample holder with high temperature uniformity and at least three identical temperature sensors. The measured actual temperatures of the sample holder are processed in order to determine if the execution of the temperature profile should be continued or aborted. Furthermore, temperature sensors which measure actual temperatures that do not fulfil certain requirements are excluded from further monitoring and controlling the temperature of a sample holder.

Provided is a temperature control system applied to an apparatus for analyzing a sample using a pipette nozzle and a reaction container, including a pipette tip temperature controller that heats a pipette tip which is fitted on the pipette nozzle and aspirates or discharges liquid and a reaction container temperature controller that heats the reaction container. The pipette tip temperature controller heats, in a concentrated manner, at least a distal end portion of the pipette tip of the pipette nozzle located at a predetermined heating position with hot air emitted from a heat source and is configured such that a distal end is capable of arriving at the reaction container by lowering the pipette nozzle from the heating position.

Diagnostic systems for sensing biological molecules are disclosed. The diagnostic systems include a fluid control delivery and control system that can be coupled to a diagnostic cartridge that includes a microfluidic device and integrated sensing electronics. The diagnostic systems can be used to sequence biological molecules.

A nucleic acid amplification in-situ real-time detection system and method using a micro-fluidic chip through optical fiber sensing. The system includes a white light source, a detection optical path, a microfluidic chip and a spectrum acquisition, processing and display module, which are connected in sequence. The detection optical path is configured to transmit white light from the white light source to the micro-fluidic chip and transmit an optical signal made by the microfluidic chip to the spectrum acquisition, processing and display module. The micro-fluidic chip is configured to carry out biochemical reaction; the spectrum acquisition, processing and display module is configured to acquire the optical signal, analyze the signal and generate a visual biochemical reaction real-time dynamic-change signal curve. This microfluidic chip real-time detection device detects nucleic acid amplification information by using a white light interfered hyperspectral method, so fluorescence-labeled analyte and non-fluorescence-labeled analyte are detected.

The present disclosure, in some aspects, provides devices useful for determining a target nucleic acid profile of a sample. The device comprises an enrichment module, a reaction module and a nucleic acid sequencer. In other aspects, the present disclosure provides kits, components and compositions (such as consumables) of the devices and methods of using the devices described herein.

A detection chip, a using method for the same, and a reaction system. The detection chip includes a first substrate, a micro-cavity defining layer, and a heating electrode. The micro-cavity defining layer is on the first substrate and defines a plurality of micro-reaction chambers. The heating electrode is on the first substrate and is closer to the first substrate than the micro-cavity defining layer, and is configured to heat a plurality of micro-reaction chambers. The orthographic projection of the plurality of micro-reaction chambers on the first substrate is within the orthographic projection of the heating electrode on the first substrate.

A system and method facilitates transfers of specimen containers (e.g., vials with caps) between storage cassettes and carrier cassettes. The storage cassettes are designed to be stored in cryogenic refrigerators while the carrier cassettes are designed to be temporarily stored in a portable carrier. Identification information is read from wireless transponders carried by the specimen containers. Visual mappings of the positions of the specimen container in the cassettes is provided. Presence and position of the specimen containers in the cassettes is verified, and alerts of inconsistencies provided along with corrective commands. Inventories of specimen container and even specific specimen holders are provided.

Disclosed is a chemiluminescence measurement apparatus that includes: a support member configured to support a cartridge for measuring a test substance contained in a specimen by chemiluminescence measurement; a motor configured to rotate the support member so as to rotate the cartridge such that a process required for the chemiluminescence measurement proceeds in the cartridge; and a light receiver configured to receive light generated by chemiluminescence in the cartridge that is supported by the support member rotated by the motor. The cartridge supported by the support member and a light receiving surface of the light receiver are disposed inside a dark space surrounded by a light-shielding portion, and the motor is disposed outside the dark space.

A device for thermal treatment of samples includes: a base unit with a receiving region for a sample carrier; a temperature control block arranged in the receiving region; a lid; a temperature sensor for detecting a temperature of the temperature control block; a control unit for heating and cooling the temperature control block; and a reference element for in situ calibrating, validating and/or adjusting of the temperature sensor, which reference element is comprised of a material having at least one phase change at at least one predetermined phase change temperature in a temperature range suitable for calibrating the temperature sensor, during which phase change the material remains in the solid state.